FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001818204

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001818204
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences956468
Sequences flagged as poor quality0
Sequence length151
%GC45

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA22740.2377497208479531No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC18780.1963473947899982No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA18300.1913289310253976No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC17070.1784691176286086No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA16520.17271879456500375Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC15230.15923167319763964No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC14950.15630423600162263No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC12530.1310028145217613No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT11790.12326601621800207Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC11780.12316146488957289Clontech Universal Primer Mix Long (96% over 25bp)
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA11620.12148864363470602Clontech SMART CDS Primer II A (96% over 26bp)
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT10270.10737421429676684No Hit
ATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTC10050.1050740850713249No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)