FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001818947

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001818947
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1866356
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72970.3909757838268798No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53820.28836942148228956No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA29950.16047313588618678Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26840.14380964832004184No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT24730.13250419534108177Clontech Universal Primer Mix Long (96% over 26bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23490.12586023245297254No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)