FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001819821

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001819821
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1673373
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAATAGCGGTGTCTGGTTTCAAATCTGGGGCATTGCAACGCAACACT80640.48190092704973725No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73850.4413241996853063No Hit
CTTCTATATCAATGCTCTCAGCAGTCGCTAAGTTTAATAACTTAACGCTT65340.39046883151574696No Hit
ATCCAAACGCGATCCTTTCTTTCCAAGATAAAGTTATCCTTGAATAAAAA47380.2831406984575465No Hit
TGCTAAAGTGGTCCACGATGCCAAACTTGAAAGTTTGATGGAACTTGAAA44890.2682605731059363No Hit
ATCATATATGCACTTTATGTGCCGATGGTTTTGCCTACTACGTCAGCCTT44550.26622874876073654No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42490.25391828361040847No Hit
ATATAGAAGATGACATGAACGCCGCACGTGCTTCGTTATTTGTAACGAGG42100.25158766156738516No Hit
TATATGCACTTTATGTGCCGATGGTTTTGCCTACTACGTCAGCCTTAGCA40220.24035286812922166No Hit
CTTAAGGAGCTTGCTAAAGTGGTCCACGATGCCAAACTTGAAAGTTTGAT39520.23616970035969267No Hit
CATATATGCACTTTATGTGCCGATGGTTTTGCCTACTACGTCAGCCTTAG38850.2321658112088578No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36640.2189589529650592No Hit
CTATGTGCACTCCGTCTTGATCCTTATGAATCGAGCGGGGCCACCTCGTT36390.2174649644759417No Hit
GCGTTGATACCACTGCTTAGCGGTGATGAAATTATTGTTGATATGCCTTC35890.21447698749770672No Hit
TTTGTGGATCATGCAATCATATATGCACTTTATGTGCCGATGGTTTTGCC35100.20975598387209549No Hit
TCTTTAAACTTGCCCTTTGGGTAGATAAGTGAGCTAATATGGCGTCTAGT34480.2060508924190841No Hit
ACACTATGTGCACTCCGTCTTGATCCTTATGAATCGAGCGGGGCCACCTC32870.19642960654916744No Hit
ATCCACAAATATATCCTCCTTTTTATTCAAGGATAACTTTATCTTGGAAA32740.19565273253482635No Hit
CTCTTAAGGAGCTTGCTAAAGTGGTCCACGATGCCAAACTTGAAAGTTTG30700.18346178646362765No Hit
CCTTAAGAGCTTGATGGTCCACTTGGAAACTGGTGCTTGGCCTTGTGTCC30350.1813702025788632No Hit
ATATATTTGTGGATCATGCAATCATATATGCACTTTATGTGCCGATGGTT29160.1742588173706639No Hit
GCATATATGATTGCATGATCCACAAATATATCCTCCTTTTTATTCAAGGA28620.17103180223417014No Hit
CAAATATATCCTCCTTTTTATTCAAGGATAACTTTATCTTGGAAAGAAAG28500.17031468775939373No Hit
ATATATGATTGCATGATCCACAAATATATCCTCCTTTTTATTCAAGGATA28270.16894021834940565No Hit
AATATATCCTCCTTTTTATTCAAGGATAACTTTATCTTGGAAAGAAAGGA26370.15758590583211274No Hit
GTACATGGGGTTATCATAACCACACACTGAAATTAAACTTGCCCGTTGGG24430.14599255515656104No Hit
AAATAATACCACGGACACAAGGCCAAGCACCAGTTTCCAAGTGGACCATC24150.14431928804874944No Hit
CCAATAGCGGTGTCTGGTTTCAAATCTGGGGCATTGCAACGCAACACTAT24100.14402049035092596No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23800.14222770416398495No Hit
ACTTTAGCAAGCTCCTTAAGAGCTTGATGGTCCACTTGGAAACTGGTGCT23690.14157034922877326No Hit
AGCTAATATGGCGTCTAGTGGAAATAATACCACGGACACAAGGCCAAGCA22630.13523583803491512No Hit
GCATATCACTAGAATTCTTCATAATTCTAGCGTAGCAGCATTTGGTTTCA22500.13445896402057403No Hit
GGATATATTTGTGGATCATGCAATCATATATGCACTTTATGTGCCGATGG22380.13374184954579763No Hit
GTACATGGGGGTTATCATAACCACACACTGAAATTAAACTTGCCCGTTGG22120.13218810151711544No Hit
ACGCAACACTATGTGCACTCCGTCTTGATCCTTATGAATCGAGCGGGGCC21770.13009651763235094No Hit
AAATAGAATCATCCCAAAGAAAATACCACACTCCAATAGCGGTGTCTGGT21760.13003675809278625No Hit
TCTCATATCTTTGCTTAGAGAAAGAAATAGAATCATCCCAAAGAAAATAC21010.1255547926254338No Hit
CTTATGAATCGAGCGGGGCCACCTCGTTACAAATAACGAAGCACGTGCGG20920.12501695676935148No Hit
ACTATGTGCACTCCGTCTTGATCCTTATGAATCGAGCGGGGCCACCTCGT20920.12501695676935148No Hit
TTCTAGTGATATGCAATGCCTGGATCTTCGATCGATCCAAACGCGATCCT20650.1234034492011046No Hit
GTTTAATAACTTAACGCTTGCTAAGGCTGACGTAGTAGGCAAAACCATCG20440.1221484988702459No Hit
GTCTTGATCCTTATGAATCGAGCGGGGCCACCTCGTTACAAATAACGAAG20420.12202897979111652No Hit
ATGTGCACTCCGTCTTGATCCTTATGAATCGAGCGGGGCCACCTCGTTAC20180.12059475084156372No Hit
CTTTAGCAAGCTCCTTAAGAGCTTGATGGTCCACTTGGAAACTGGTGCTT19430.11611278537421124No Hit
TCACTAGAATTCTTCATAATTCTAGCGTAGCAGCATTTGGTTTCAAGTTC19300.11533591135987015No Hit
TGCTAAGGCTGACGTAGTAGGCAAAACCATCGGCACATAAAGTGCATATA18500.1105551481946942No Hit
GAAATAGAATCATCCCAAAGAAAATACCACACTCCAATAGCGGTGTCTGG18370.1097782741803531No Hit
CGTTTGGATCGATCGAAGATCCAGGCATTGCATATCACTAGAATTCTTCA18200.10876236200775322No Hit
CTCCTTAAGAGCTTGATGGTCCACTTGGAAACTGGTGCTTGGCCTTGTGT18140.10840380477036501No Hit
GTGCATATATGATTGCATGATCCACAAATATATCCTCCTTTTTATTCAAG18010.10762693075602392No Hit
TTGCTAAAGTGGTCCACGATGCCAAACTTGAAAGTTTGATGGAACTTGAA17960.10732813305820042No Hit
TCTATATCAATGCTCTCAGCAGTCGCTAAGTTTAATAACTTAACGCTTGC17950.10726837351863572No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17830.10655125904385931No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17810.10643173996472992No Hit
TGCCTACATTGTTCCATCGACCAGAGGCTGTTCACCTTGGAGACCTGATG17440.10422063700083603No Hit
GTTTCCAAGTGGACCATCAAGCTCTTAAGGAGCTTGCTAAAGTGGTCCAC16810.10045578600825997No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)