FastQCFastQC Report
Wed 8 May 2024
EGAF00001820962

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001820962
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences848600
Total Bases128.1 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC46

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA11640.13716709875088381No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA9840.11595569172755127Clontech SMART CDS Primer II A (96% over 26bp)
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC9770.11513080367664388No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC9180.108178175818996Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC8790.1035823709639406No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA8720.10275748291303324No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA8600.10134338911147772Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC8550.10075418336082959No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)