FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001821639

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001821639
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2189408
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63150.28843413379324456No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA47080.21503529721276252Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41660.19027974685394408No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT40040.18288048641459242Clontech Universal Primer Mix Long (96% over 26bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30360.13866762156710855No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26450.12080891272892033No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)