FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001822396

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001822396
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1293873
Sequences flagged as poor quality0
Sequence length151
%GC44

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA25560.19754643616490952No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC21400.1653949035183515No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC20950.16191697330418056No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC20690.15990750251377067Clontech Universal Primer Mix Long (96% over 25bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA19960.15426552683300448Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA19470.15047844726646278No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC18960.1465367930237357No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA17590.1359484277050375Clontech SMART CDS Primer II A (96% over 26bp)
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC16730.12930171662906637No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC13920.10758397462502117No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT13460.10402875707275752No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT13320.1029467343394599Clontech Universal Primer Mix Long (96% over 26bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)