FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001822427

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001822427
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2181369
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90170.4133642680353484No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62410.28610473514568147No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA62230.285279565263832Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA60380.2767986525892685Clontech SMART CDS Primer II A (96% over 26bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC55760.2556192922884665Clontech Universal Primer Mix Long (96% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT47300.21683630784154356Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34610.15866183117115903No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT34010.1559112648983276Clontech Universal Primer Mix Long (96% over 26bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33210.15224384320121903No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG29950.13729909978550167Clontech SMART CDS Primer II A (96% over 26bp)
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA29730.13629055881879681Clontech Universal Primer Mix Long (96% over 25bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG27890.12785548891544712Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26470.12134581540307944No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)