FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001823005

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001823005
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2338
Sequences flagged as poor quality0
Sequence length151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT230.9837467921300257No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170.7271171941830624No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140.5988023952095809No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50.21385799828913601No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT30.1283147989734816Clontech Universal Primer Mix Long (96% over 26bp)
CTGTTATTCCACCAAAACCAAATCAGATAAAAAAGTGAGGGCTTATCTAG30.1283147989734816No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.1283147989734816No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)