FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001823009

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001823009
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1971
Sequences flagged as poor quality0
Sequence length151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT261.3191273465246067No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140.7102993404363267No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130.6595636732623034No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA60.30441400304414Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCATTAACCAGTCTTTTACTACTAAACTTAAATGGCCAATTGAAACAAAC30.15220700152207No Hit
TACAAGTCTTAACCACAGTGTAACTTACAGTTTCCTTTAAAAAAAAAAAA30.15220700152207No Hit
TCCTAGGGTTTATCGTGTGAGCACACCATATATTTACAGTAGGAATAGAC20.10147133434804667No Hit
ATCTAGAATTTATTTCCTTCATTAAGAAATAATTTGAACTTAACTGTAAC20.10147133434804667No Hit
CTCTCAGCCGATGAACAGTTGGAATAGGTTGTTAGCGGTAACTAAGATTA20.10147133434804667No Hit
GTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGC20.10147133434804667No Hit
CCTTAAATTTCCTTCGGGGCCATACAACACTTCTGGAGAAGGGATTTGGG20.10147133434804667No Hit
GTGTCCCGCGGGGCCCGAAGCGTTTACTTTGAAAAAATTAGAGTGTTCAA20.10147133434804667No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT20.10147133434804667Clontech Universal Primer Mix Long (96% over 26bp)
GTGTCCGTGTGCTTCAAGATGCGCATGTCTTTCATGGAGTATGAACAGTA20.10147133434804667No Hit
ACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACC20.10147133434804667No Hit
CTCTAAATTGCTGCACTCCTGTAACAAACATTATTTTTCCATTTCATTGT20.10147133434804667No Hit
CCTTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGT20.10147133434804667No Hit
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG20.10147133434804667Clontech Universal Primer Mix Long (96% over 26bp)
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.10147133434804667No Hit
CCTTAATAGCTACCCAACAAGTCATTAACATACAGAAACATGCATCATGA20.10147133434804667No Hit
ATGTTATATGATTAGTTTGCTATTTGTAAAGTTACACTTGACCATCTTTA20.10147133434804667No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATT20.10147133434804667No Hit
GTTAACTTATGCACGCTTAACTATCTTAACAAGCTTTGAGTGCAAGAGAT20.10147133434804667No Hit
GACCAGACTTCGCTCGTACTCGTGCGCCTCGCTTCGCTTTTCCTCCGCAA20.10147133434804667No Hit
CCCACATACCATGCACCACGGGTGCTATGCCGCTTCTTACAGGACCTTTT20.10147133434804667No Hit
GTACATGGGTGATATATAAACTCAGACCCAAACATTAATCAGTTCTTCAA20.10147133434804667No Hit
CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGG20.10147133434804667No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG20.10147133434804667Clontech Universal Primer Mix Long (96% over 26bp)
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.10147133434804667No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.10147133434804667No Hit
TGTGAGATGAGCATTCCTGATAAGTGGTCATCCAATTTGGAAAGACAGTA20.10147133434804667No Hit
GATATTTCCCTGGGATCCAGCTGGAGATGTTGGGCTGGGAGGCCCTGTGG20.10147133434804667No Hit
CCTTATTCGAGCCGAGCTGGGCCAGCCAGGCAACCTTCTAGGTAACGACC20.10147133434804667No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.10147133434804667No Hit
GTATGTAAATTGGAAGTTAACTTATGCACGCTTAACTATCTTAACAAGCT20.10147133434804667No Hit
GACTGAACCACTTCCAGGAGTGCCTTGATCACCAGCTTAATGGTCAGATC20.10147133434804667No Hit
TTATGAAAGTGTCAGTGAAACAATTACTAAGATGCTGAAGGCAATAGAAG20.10147133434804667No Hit
GGGGAACATTCTCATGGAGGTACTGTAAGGCTAAGTCTGTCCACTTCATT20.10147133434804667No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA20.10147133434804667No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.10147133434804667No Hit
GCTATTATTCATCCTATGTGGGTAATTGAGGAGTATGCTAAGATTTTGCG20.10147133434804667No Hit
AGGTGGGTAGCATCATAAGCCTTCCATTTACTTTGATCTGTTCCCAAATA20.10147133434804667No Hit
GAGTCTCACAGCTTGTAAAGCCTGAGACAGCTGCCTTGTGTGCGACTGAG20.10147133434804667No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)