FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001823010

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001823010
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1971
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATATAGTCACTCCAGGTTTATGGAGGGTTCTTCTACTATTAGGACTT30.15220700152207No Hit
ATATGAGACAGACAGTTGCGGTGGGTGTCATCAAAGCAGTGGACAAGAAG30.15220700152207No Hit
GTGGTAGAATGAGGACTTATGCAAGGTATAAATGCGCATAGCATTTTACT30.15220700152207No Hit
TTCCTGTTCTGTGGCTTCTTTTAGTGCCCAAGTTGTATCACATTTCTTGC20.10147133434804667No Hit
TATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGGG20.10147133434804667No Hit
GTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGC20.10147133434804667No Hit
AGATGGAAGGATCTTGGATTTGATTGATGATGCTTGGCGAGAAGACAAGC20.10147133434804667No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT20.10147133434804667Clontech Universal Primer Mix Long (96% over 26bp)
TTCCCGTACTTCTGTCTTCCAGTTTTCCACTTCAAACTTCTATCTTCTCC20.10147133434804667No Hit
GATGAGAGCTGGGACTTTGGCACTGGTGCTGGATTTTATGTTGATGCCAC20.10147133434804667No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA20.10147133434804667No Hit
GACAGGGTAACATCTTTGAAATCAGGCCTCCGTGGTCAGGAGCTTTGTCT20.10147133434804667No Hit
GCTTCCAGCTCCTCCCTGGAGAAGAGCTACGAGCTGCCTGACGGCCAGGT20.10147133434804667No Hit
ATTCAGAAAGACGCCCTGGAGCCAGGACAGAGGGTGGTCGTCGTGGATGA20.10147133434804667No Hit
ATTATAACCCTACATTTTGTGCATAAAGTGTAAGTGTATAAGCATATCAA20.10147133434804667No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAACCGCGGTC20.10147133434804667No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG20.10147133434804667Clontech Universal Primer Mix Long (96% over 26bp)
GAATAGGGGAAATCAGTGAATGAAGCCTCCTATGATGGCAAATACAGCTC20.10147133434804667No Hit
CTCCATGTTTGATGTATCTGAGCAGGTTGCTCCACAGGTAGCTCTAGCAG20.10147133434804667No Hit
GAAATAAGGCTGCCATAAATTCCTCTTCAGGTTTGATCTACTTCTAGAAC20.10147133434804667No Hit
TAATAAAACTATCCTCAAGGAAAAAATTATAAGAGACCATTAACAGCCAA20.10147133434804667No Hit
AGAAATGGGTGTTCATTGAAGACTTTTCCAGAATGGTTAGAGTCATACAG20.10147133434804667No Hit
CTATACTACTAACAGACCGCAACCTCAACACCACCTTCTTCGACCCCGCC20.10147133434804667No Hit
CGTTTAGAAGGGCTATTTGTTGTGGGTCTCATGAGTTGGAGTGTAGGATA20.10147133434804667No Hit
GAATAACGCCGCCGCATCGCCGGTCGGCATCGTTTATGGTCGGAACTACG20.10147133434804667No Hit
CCCGCTACATCGCCAGTCTGAAGCAGCGTTATACGCAGAGCAATGGGCGC20.10147133434804667No Hit
CTCTAATGCCTGAGTGTGCAGCAGAGCCAAGAGAAAGAAGAAGATTTGCA20.10147133434804667No Hit
GACACAGAGGTGGACTGTTTCTCTACCTCCTCACATTGTGCTAACAGGGA20.10147133434804667No Hit
GTGTAGGCGAATAGGAAATATCATTCGGGCTTGATGTGGGGAGGGGTGTT20.10147133434804667No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG20.10147133434804667Clontech Universal Primer Mix Long (96% over 26bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)