FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001824316

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001824316
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2006
Sequences flagged as poor quality0
Sequence length151
%GC47

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT30.14955134596211367Clontech Universal Primer Mix Long (96% over 26bp)
TTTCTAATCAGAACACACTATTGCCAGGAACACAGTAGTTATTGTTAAAA30.14955134596211367No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA30.14955134596211367Clontech SMARTer II A Oligonucleotide (100% over 25bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)