FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001824701

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001824701
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2014333
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105380.5231508395086613No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68440.33976507360004526No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40870.2028959462015466No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA28980.14386896307611502Clontech SMART CDS Primer II A (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA25450.12634455176974213Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC22720.11279167843648494Clontech Universal Primer Mix Long (96% over 25bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22610.11224559196518152No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT21710.10777761174542641Clontech Universal Primer Mix Long (96% over 26bp)
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC20200.10028133382117058No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)