FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001826004

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001826004
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1628190
Sequences flagged as poor quality0
Sequence length151
%GC49

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTATACTCGGCCGTCGGGGCAAGTGCCATGCTCCGATGAGTAGGAGGA49650.3049398411733274No Hit
ATCCTAAGAGATGGGGAAGCCCTGTTTCAAGGGCGCACTTCGCGCGATCA42400.26041186839373787No Hit
TACCTACATTGTTCTATTGACCAGAGGCTGTTCACCTTGGAGACCTGATG39940.2453030665954219No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39760.24419754451261833No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAGCATCCGCA33170.2037231527033086No Hit
GTACTCGGTCCTCCAGATTTTCAAGGGCCGCCGAAGGCGCACCGGACACC31620.19420337921249978No Hit
GGTTAATATTCCCGAACCGGGACGTGGCGGCGGACGGCAACGTTAGGAAA29030.1782961447988257No Hit
CCCCTAGGCCCAGCCCTCAGAGCCAATCCTTTTCCCGAAGTTACGGATCC28440.17467248908296942No Hit
GTTCTATTGACCAGAGGCTGTTCACCTTGGAGACCTGATGCGGTTATGAG27560.16926771445592959No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27410.16834644605359325No Hit
AGTATAGGTTGCGCGCTTCAGCGCCATCCATTTTCGGGGCTAGTTGATTC24840.15256204742689736No Hit
GTACATGGGCAGAAATCACATTGCGTCAGCATCCGCAGGGACCATCGCAA24630.15127227166362647No Hit
GAGTAGGAGGACGCGGGGGTTGTTGCGAAACCTTGGGCGCGAGCCTGGGT23260.14285802025562128No Hit
TATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAGCATCCGCAG23190.14242809500119763No Hit
CCTAGGGAGAGCCCCCACGAGGGGAGCGTCCTGCGTCCGTCGCCCGATCG22920.14076981187699225No Hit
CCCCTTGTCCGTACCAGTTCTGAGTCAGCTGTTCGCCGCCTAGGGAGAGC22710.13948003611372137No Hit
TCGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTACCA20990.12891615843359805No Hit
ACCGAGTACCGTTCACGCCCGGTCGTACTCATAACCGCATCAGGTCTCCA20790.12768780056381626No Hit
GAGTACGACCGGGCGTGAACGGTACTCGGTCCTCCAGATTTTCAAGGGCC20650.12682795005496902No Hit
GTCGTACTCATAACCGCATCAGGTCTCCAAGGTGAACAGCCTCTGGTCAA20530.12609093533309995No Hit
ATGTAGGTAAGGGAAGTCGGCAAAATGGATCCGTAACTTCGGGAAAAGGA20020.1229586227651564No Hit
GTTTCAAGGGCGCACTTCGCGCGATCATCGAAAGGGAATCGGGTTAATAT19760.12136175753444008No Hit
ACTCATAACCGCATCAGGTCTCCAAGGTGAACAGCCTCTGGTCAATAGAA19610.12044048913210374No Hit
GTTATGAGTACGACCGGGCGTGAACGGTACTCGGTCCTCCAGATTTTCAA19330.11872078811440925No Hit
GACCAGAGGCTGTTCACCTTGGAGACCTGATGCGGTTATGAGTACGACCG19250.11822944496649654No Hit
TCCCTTACCTACATTGTTCTATTGACCAGAGGCTGTTCACCTTGGAGACC18250.11208765561758763No Hit
GATTAAGACAGCAGGACGGTGGTCATGGAAGTCGAAATCCGCTAAGGAGT18220.11190340193712035No Hit
CAATAGAACAATGTAGGTAAGGGAAGTCGGCAAAATGGATCCGTAACTTC18200.11178056615014219No Hit
ATATTAACCCGATTCCCTTTCGATGATCGCGCGAAGTGCGCCCTTGAAAC17730.108893925156155No Hit
ACATTGTTCTATTGACCAGAGGCTGTTCACCTTGGAGACCTGATGCGGTT17660.10846399990173136No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17110.10508601575983147No Hit
TACCAGTTCTGAGTCAGCTGTTCGCCGCCTAGGGAGAGCCCCCACGAGGG16840.10342773263562607No Hit
TTGTTCTATTGACCAGAGGCTGTTCACCTTGGAGACCTGATGCGGTTATG16560.10170803161793157No Hit
TCCTAACGTTGCCGTCCGCCGCCACGTCCCGGTTCGGGAATATTAACCCG16550.10164661372444248No Hit
CCCTGAAATCGGTTCAACCGGAGATAGGGTCCAGCGGCTGGAAGAGCACC16420.10084818110908432No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)