FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001826441

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001826441
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2265808
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125610.5543717737778312No Hit
GTAATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGAAACA101100.44619844223341076No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85370.3767750842083707No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC77880.34371844392817047No Hit
TCATAAGACTGACAGTAATAGTCAGCCTCGTCCTCAGTCTTCAGCCCAGA73640.32500547266140817No Hit
TTTAAAAGGTGTCCAGTGTGAGGTGCAGCTCTTGGAGTCTGGGGGAGGCT67540.2980835092823399No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG66340.29278738533891663No Hit
ACTATGTACAGTGGTACCAGCAGCGCCCGGGCAGTGCCCCCACCACTGTG57810.2551407709744162No Hit
GTAATAGTCAGCCTCGTCCTCAGTCTTCAGCCCAGAGATGGTGAGGGAGG51130.22565901435602662No Hit
ACCAAAGACCCTCTGGGGTCCCTGATCGGTTCTCTGGCTCCATCGACATC49860.22005394984923704No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT46610.20571028083579898No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46120.20354769689223448No Hit
GACCTAGGACGATCAGCTTGGTCCCTCCGCCGAACACCTGATTGTTGCCA44540.19657446703339382No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG43500.19198449294909367No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41880.18483472562547223No Hit
GTACATAGTTGCTGGCAATACTGCCACTGCTGCGGGTGCAGGAGATGGTT41370.18258387294951733No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT39850.17587544928784787No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT39190.17296258111896506No Hit
CCACTATGGCCTGGGCTCCACTACTTCTCACCCTCCTCGCTCACTGCACA38710.17084413154159578No Hit
GTATATTACTGTGCGAGGAGCCTAGGGACCTATTATGATTTTGAGGTTGC38660.1706234597106198No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG37680.16629829182349076No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA36940.16303234872504643No Hit
ATGTACAGTGGTACCAGCAGCGCCCGGGCAGTGCCCCCACCACTGTGATC36930.16298821435885125No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG36720.16206139266875216No Hit
ATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGAAACAGCG36670.1618407208377762No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT36430.16078149604909156No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC35820.1580892997111847No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA35720.15764795604923276No Hit
CAGTAATAGTCAGCCTCGTCCTCAGTCTTCAGCCCAGAGATGGTGAGGGA35500.1566769999929385No Hit
ATATTACGCAGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGACAATT34870.15389653492264127No Hit
ATTTTATGCTGACTCAGCCCCACTCTGTGTCGGAGTCTCCGGGGAAGACG34320.15146914478190562No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCA34190.1508953980213681No Hit
GCCTTGGGCTGACCTAGGACGATCAGCTTGGTCCCTCCGCCGAACACCTG33910.14965963576790267No Hit
GCCCCACTCTGTGTCGGAGTCTCCGGGGAAGACGGTAACCATCTCCTGCA33480.14776185802150932No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG32130.14180371858515814No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG31460.13884671605008014No Hit
GTCTTATGATGGCAACAATCAGGTGTTCGGCGGAGGGACCAAGCTGATCG31110.13730201323324837No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA30800.13593384788119733No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGC30310.13377126393763283No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29950.13218242675460584No Hit
TCTCTGGGCTGAAGACTGAGGACGAGGCTGACTATTACTGTCAGTCTTAT29510.13024051464201733No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA29020.12807793069845283No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT28430.12547400309293638No Hit
GACTATTACTGTCAGTCTTATGATGGCAACAATCAGGTGTTCGGCGGAGG28250.12467958450142289No Hit
CTATTACTGTCAGTCTTATGATGGCAACAATCAGGTGTTCGGCGGAGGGA27940.12331141914937188No Hit
ACTATGGCCTGGGCTCCACTACTTCTCACCCTCCTCGCTCACTGCACAGG27270.12035441661429389No Hit
ATATACACTACCACCACTTCCACTAATAGCTGAGACCCACTCCAGCCCCT27090.1195599980227804No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCT26930.1188538481636573No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT26880.11863317633268133No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC26610.11744154844541108No Hit
GGTACCAGCAGCGCCCGGGCAGTGCCCCCACCACTGTGATCTATGAGCAT26110.11523483013565139No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGG26090.115146561403261No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTT25820.11395493351599076No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25660.11324878365686766No Hit
GTGTCGGAGTCTCCGGGGAAGACGGTAACCATCTCCTGCACCCGCAGCAG24930.11002697492461852No Hit
GTCTTTGGTTATGCTCATAGATCACAGTGGTGGGGGCACTGCCCGGGCGC24830.10958563126266656No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA24800.10945322816408098No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCC24020.10601074760085585No Hit
GTACATGGGAGCTCTGAGAGAGGAGCCCAGCCCTGGGATTTTCAGGTGTT23780.10495152281217121No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG23660.10442191041782888No Hit
GTACAGTGGTACCAGCAGCGCCCGGGCAGTGCCCCCACCACTGTGATCTA23200.10239172957284995No Hit
GCCGTATATTACTGTGCGAGGAGCCTAGGGACCTATTATGATTTTGAGGT22700.10018501126309025No Hit
GCGTAATATACACTACCACCACTTCCACTAATAGCTGAGACCCACTCCAG22680.10009674253069986No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)