FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001826442

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001826442
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2265808
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGAAACA96970.4279709489947957No Hit
TCATAAGACTGACAGTAATAGTCAGCCTCGTCCTCAGTCTTCAGCCCAGA73180.32297529181642926No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC70520.3112355504085077No Hit
TTTAAAAGGTGTCCAGTGTGAGGTGCAGCTCTTGGAGTCTGGGGGAGGCT66940.29543544731062826No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG64620.28519627435334327No Hit
ACTATGTACAGTGGTACCAGCAGCGCCCGGGCAGTGCCCCCACCACTGTG56680.2501535875943593No Hit
GTAATAGTCAGCCTCGTCCTCAGTCTTCAGCCCAGAGATGGTGAGGGAGG51860.22888082308827581No Hit
ACCAAAGACCCTCTGGGGTCCCTGATCGGTTCTCTGGCTCCATCGACATC48950.21603772252547435No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT45320.20001694759661898No Hit
GACCTAGGACGATCAGCTTGGTCCCTCCGCCGAACACCTGATTGTTGCCA44140.19480909238558608No Hit
GTACATAGTTGCTGGCAATACTGCCACTGCTGCGGGTGCAGGAGATGGTT41800.1844816506959107No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG40760.1798916766116105No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT39260.17327152168233143No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA37630.1660776199925148No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT36620.16162004900680021No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG36280.16011948055616362No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG36120.15941333069704053No Hit
GTATATTACTGTGCGAGGAGCCTAGGGACCTATTATGATTTTGAGGTTGC35850.1582217028097703No Hit
CAGTAATAGTCAGCCTCGTCCTCAGTCTTCAGCCCAGAGATGGTGAGGGA35590.15707420928869525No Hit
ATGTACAGTGGTACCAGCAGCGCCCGGGCAGTGCCCCCACCACTGTGATC35350.15601498450001058No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA35190.1553088346408875No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC34980.15438201295078843No Hit
CCACTATGGCCTGGGCTCCACTACTTCTCACCCTCCTCGCTCACTGCACA34850.1538082661902509No Hit
ATTTTATGCTGACTCAGCCCCACTCTGTGTCGGAGTCTCCGGGGAAGACG34120.15058645745800175No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCA34010.1501009794298546No Hit
GCCTTGGGCTGACCTAGGACGATCAGCTTGGTCCCTCCGCCGAACACCTG33520.1479383954862901No Hit
ATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGAAACAGCG33290.14692330506380066No Hit
ATATTACGCAGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGACAATT32410.14303948083862358No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT32230.14224506224711006No Hit
GCCCCACTCTGTGTCGGAGTCTCCGGGGAAGACGGTAACCATCTCCTGCA31020.13690480393749163No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG30100.13284444224753378No Hit
TCTCTGGGCTGAAGACTGAGGACGAGGCTGACTATTACTGTCAGTCTTAT30070.1327120391489482No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA28650.12644495914923065No Hit
GTCTTATGATGGCAACAATCAGGTGTTCGGCGGAGGGACCAAGCTGATCG28580.1261360185858643No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG28050.12379689717751902No Hit
CTATTACTGTCAGTCTTATGATGGCAACAATCAGGTGTTCGGCGGAGGGA27490.12132537267058816No Hit
GACTATTACTGTCAGTCTTATGATGGCAACAATCAGGTGTTCGGCGGAGG27330.12061922281146505No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA27330.12061922281146505No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT26570.1172650109806303No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT26340.11624992055814085No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC25440.1122778276005734No Hit
ACTATGGCCTGGGCTCCACTACTTCTCACCCTCCTCGCTCACTGCACAGG25430.1122336932343782No Hit
GTCTTTGGTTATGCTCATAGATCACAGTGGTGGGGGCACTGCCCGGGCGC25370.11196888703720705No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGG25350.11188061830481665No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTT25030.11046831858657045No Hit
ATATACACTACCACCACTTCCACTAATAGCTGAGACCCACTCCAGCCCCT24980.11024764675559447No Hit
GGTACCAGCAGCGCCCGGGCAGTGCCCCCACCACTGTGATCTATGAGCAT24330.10737891295290686No Hit
GTGTCGGAGTCTCCGGGGAAGACGGTAACCATCTCCTGCACCCGCAGCAG24290.10720237548812608No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG23920.10556940393890392No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA23630.10428950731924329No Hit
GTACAGTGGTACCAGCAGCGCCCGGGCAGTGCCCCCACCACTGTGATCTA23550.10393643238968174No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCT23370.10314201379816824No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)