FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001826989

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001826989
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1531
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100.6531678641410843No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70.45721750489875895No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50.32658393207054215No Hit
GCATCATGGAGGTTTGAAGATGCCGCATTTGGATTGGATGAATTCCAAAT20.13063357282821686No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAG20.13063357282821686No Hit
GCTTAGAATAACATCCTCCTTTGGGAGGTGGTCTTGGGTGCGTGGATGTT20.13063357282821686No Hit
AAATAATAAGTAATTGAATTATTTCAGTTTAGTTACACAGCATCTTTTAA20.13063357282821686No Hit
GGGCTGGGGGGATCCCAGCAGGGGGAGGGCAATCCCTTCACCCCAGTTGC20.13063357282821686No Hit
GTGTATAAGCATATCAATATTAAAAAGCAAGCAAGCAGAATTTGGAATTC20.13063357282821686No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA20.13063357282821686Clontech Universal Primer Mix Long (96% over 26bp)
GTATTAGCAAACTCATCACTAGACATCGTACTACACGACACGTACTACGT20.13063357282821686No Hit
GAGATGTAGCACTCACATGCTTTATTTCCTAAATAATTTATAACCTCAGG20.13063357282821686No Hit
GCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGG20.13063357282821686No Hit
TATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC20.13063357282821686No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA20.13063357282821686No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTT20.13063357282821686No Hit
CTCGATGCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTACTTTGA20.13063357282821686No Hit
GTATCACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.13063357282821686No Hit
GTATGGGATGGCGGATAGTAAGTTTGTAATTACTGTGGCCCCTCAGAATG20.13063357282821686No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)