FastQCFastQC Report
Sat 10 Sep 2022
EGAF00001827349

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001827349
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1664778
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57640.3462323504995861No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA39310.2361275797733992Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT35910.21570443626717797Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34100.20483211575357194No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA23710.14242139192132525Clontech SMART CDS Primer II A (96% over 26bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC20420.1226589971755994Clontech Universal Primer Mix Long (96% over 25bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17910.10758191182247724No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG17290.10385769153604865Clontech Universal Primer Mix Long (96% over 26bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)