FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001832351

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001832351
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1980968
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95660.4828952310183708No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61640.31116100815358955No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44550.22489005375149929No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36470.1841019138118334No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA34790.1756212114481405Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27330.13796285452364704No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT27040.13649892375848574Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24340.12286922353112216No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA24110.12170817499323563Clontech SMART CDS Primer II A (96% over 26bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC21470.10838135699314677Clontech Universal Primer Mix Long (96% over 25bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)