FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001832811

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001832811
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3163517
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA475841.5041487053807518Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT448001.41614538502559Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT246520.7792592864207779Clontech Universal Primer Mix Long (96% over 26bp)
GCTTAACATGTACTCTGCGTTGATACCACTGCTTAACATGTACTCTGCGT243550.7698710011673716Clontech SMART CDS Primer II A (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170160.5378823632052554No Hit
GAGTACATGTTAAGCAGTGGTATCAACGCAGAGTACATGTTAAGCAGTGG163780.5177149356238642Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG161960.5119618449971978Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG151740.47965602840130145Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120070.37954592941969334No Hit
CATGTACTCTGCGTTGATACCACTGCTTAACATGTACTCTGCGTTGATAC117710.3720858778378621Clontech SMART CDS Primer II A (96% over 26bp)
ATGTTAAGCAGTGGTATCAACGCAGAGTACATGTTAAGCAGTGGTATCAA114830.36298208607698335Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC112600.355932969539914Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC99010.3129744521682672Clontech SMART CDS Primer II A (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG90340.28556824572145495Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG83180.2629352078714924Clontech Universal Primer Mix Long (96% over 26bp)
TGTTAAGCAGTGGTATCAACGCAGAGTACATGTTAAGCAGTGGTATCAAC77030.24349481921544913Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AACATGTACTCTGCGTTGATACCACTGCTTAACATGTACTCTGCGTTGAT75220.23777333897684128Clontech SMART CDS Primer II A (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT75130.23748884548431384Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC73580.23258923533522974Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGTTAAGCAGTGGTATCAACGCAGAGTACATGTTAAGCAGTGGTA71490.2259826642309809Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71130.22484469026087103No Hit
GTTAAGCAGTGGTATCAACGCAGAGTACATGTTAAGCAGTGGTATCAACG61360.19396134112761207Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCAACGCAGAGTACATGTTAAGCAGTGGTATCAACGCAGAGTACATGT60190.1902629257247551Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA59490.1880501985606526Clontech SMART CDS Primer II A (96% over 26bp)
GCGTTGATACCACTGCTTAACATGTACTCTGCGTTGATACCACTGCTTAA59090.1867857830383083Clontech SMART CDS Primer II A (96% over 26bp)
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA52690.16655513468079988Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC50210.15871575844226535Clontech Universal Primer Mix Long (96% over 25bp)
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG49350.15599726506922518Clontech Universal Primer Mix Long (96% over 26bp)
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC47340.14964357706944517Clontech Universal Primer Mix Long (96% over 26bp)
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA45620.14420659032336478Clontech Universal Primer Mix Long (96% over 26bp)
ACTCTGCGTTGATACCACTGCTTAACATGTACTCTGCGTTGATACCACTG45140.14268929169655165Clontech Universal Primer Mix Long (95% over 23bp)
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC43200.13655687641318193Clontech Universal Primer Mix Long (96% over 26bp)
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC41130.1300135260850503Clontech Universal Primer Mix Long (96% over 26bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC40380.12764274698065475Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGTTAAGCAGTGGTATCAACGCAGAGTACAT39850.1259673964135486Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTAACATGTACTCTGCGT39830.12590417563743136Clontech SMART CDS Primer II A (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTAACATGTACTCTGCGTTGAT39480.12479781205538014Clontech SMART CDS Primer II A (96% over 26bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT36400.11506181253332921Clontech Universal Primer Mix Long (100% over 23bp)
GTATCAACGCAGAGTACATGTTAAGCAGTGGTATCAACGCAGAGTACATG36210.11446121516021568Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTGCTTAACATGTACTCTGCGTTGATACCACTGCTTAACATGTACTCTGC35860.11335485157816443Clontech SMART CDS Primer II A (96% over 26bp)
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT35830.11326002041398861Clontech Universal Primer Mix Long (96% over 26bp)
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA35800.1131651892498128Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG33610.10624251426497788Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CATGTTAAGCAGTGGTATCAACGCAGAGTACATGTTAAGCAGTGGTATCA32700.10336596895164464Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTTAACATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT32310.10213316381735899Clontech Universal Primer Mix Long (96% over 26bp)
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT32120.10153256644424545Clontech Universal Primer Mix Long (96% over 26bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)