FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001834711

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001834711
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4214566
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA1142922.7118331994326343Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT1049122.489271730469994Clontech Universal Primer Mix Long (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT976122.3160629113412865Clontech Universal Primer Mix Long (96% over 26bp)
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA548361.3011066857180549Clontech SMART CDS Primer II A (96% over 26bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC441241.0469405390732995Clontech Universal Primer Mix Long (96% over 25bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG336600.7986587468318209Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG294100.6978179959692172Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC218380.5181553687853032Clontech SMART CDS Primer II A (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG212970.5053189343813811Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT207600.49257740891944746Clontech Universal Primer Mix Long (96% over 26bp)
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC203960.48394069519850913Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG200420.4755412538325417Clontech SMART CDS Primer II A (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT192880.457650918267741No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG181160.42984259826515947Clontech Universal Primer Mix Long (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC178980.42467006092679527Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC166390.39479747143596755Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT146590.3478175451517428Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144320.3424314626939049No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA136600.32411403689015666Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTGCCATGTACTCTGCGT127200.30181043552289843Clontech Universal Primer Mix Long (96% over 28bp)
GCTTGCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT126710.3006478009835414Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT125990.29893944002775136Clontech Universal Primer Mix Long (96% over 25bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTT123440.2928889949759952Clontech SMART CDS Primer II A (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT117610.2790560166811956Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG111970.2656738558608407Clontech Universal Primer Mix Long (96% over 26bp)
ATGGCAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA111580.2647484936764545Clontech Universal Primer Mix Long (96% over 28bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC109310.2593624112186166Clontech Universal Primer Mix Long (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA108940.2584845035052245Clontech SMART CDS Primer II A (96% over 26bp)
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC107830.25585078036504827Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA101540.24092634923738293Clontech Universal Primer Mix Long (96% over 25bp)
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA100890.2393840789300725Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGCAAGCAGTGGTATCAACGCAGAGTACATGGCAAGCAGTGGTA99570.23625208384445753Clontech Universal Primer Mix Long (96% over 28bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGCAAGCAGTGGTA98600.23395054200124046Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC96150.22813736930445508Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT95610.22685609858761258Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGCAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA94080.22322583155655884Clontech Universal Primer Mix Long (96% over 28bp)
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA92340.21909729258006638Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA91420.21691438691433473Clontech Universal Primer Mix Long (96% over 26bp)
GTACATAGGAACAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT90900.21568057066848637Clontech SMARTer II A Oligonucleotide (100% over 22bp)
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC89950.2134264832962635Clontech Universal Primer Mix Long (96% over 26bp)
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA88530.2100572158556777Clontech Universal Primer Mix Long (96% over 26bp)
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG88240.20936912602626226Clontech SMARTer II A Oligonucleotide (100% over 23bp)
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA85030.20175268343169853Clontech Universal Primer Mix Long (96% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTGCCATGTACTCTGCGTTGAT84430.20032904930187356Clontech Universal Primer Mix Long (96% over 28bp)
GCTTGCCATGTACTCTGCGTTGATACCACTGCTTGCCATGTACTCTGCGT84240.19987823182742898Clontech Universal Primer Mix Long (96% over 28bp)
TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC82980.19688860015479648Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82170.19496669407953274No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC81720.193898968482164Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT79530.18870270390830277Clontech Universal Primer Mix Long (96% over 26bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT76960.18260480438555238Clontech Universal Primer Mix Long (100% over 23bp)
CATGGCAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA75130.17826272028958617Clontech Universal Primer Mix Long (100% over 26bp)
GCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT74260.17619845080133992Clontech Universal Primer Mix Long (96% over 26bp)
GCCATGTACTCTGCGTTGATACCACTGCTTGCCATGTACTCTGCGTTGAT71140.16879555332624996Clontech Universal Primer Mix Long (96% over 28bp)
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG67350.15980293107285543Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTTCCTATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT66280.15726411687466754Clontech Universal Primer Mix Long (96% over 26bp)
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC64980.1541795762600467Clontech Universal Primer Mix Long (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC64700.1535152136661284Clontech SMART CDS Primer II A (96% over 26bp)
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT64360.15270848765922754Clontech Universal Primer Mix Long (96% over 26bp)
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC62010.147132587317413Clontech Universal Primer Mix Long (96% over 25bp)
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA58690.13925514513238138Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATAGGAACAGTGGTAT57420.1362417862242518Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA55540.13178106595080016Clontech SMART CDS Primer II A (96% over 26bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG54860.1301676139369985Clontech Universal Primer Mix Long (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG54540.12940834240109184Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATGTCAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA54170.12853043468769976Clontech Universal Primer Mix Long (96% over 26bp)
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG53980.12807961721325517Clontech Universal Primer Mix Long (96% over 26bp)
ACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGC52940.12561198472155852Clontech Universal Primer Mix Long (96% over 25bp)
TATCAACGCAGAGTACATGGCAAGCAGTGGTATCAACGCAGAGTACATGG51600.12243253516494937Clontech Universal Primer Mix Long (100% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGTTCCTATGTACTCTGCGTT51000.12100890103512439Clontech SMARTer II A Oligonucleotide (95% over 24bp)
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG50960.12091399209313604Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATGGCAAGCAGTGGTATCAACGCAGAGTACATGGCAAGCAGTGGTATCAA50620.12010726608623522Clontech Universal Primer Mix Long (96% over 28bp)
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG48510.11510081939635065Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG48280.11455509297991776Clontech Universal Primer Mix Long (96% over 25bp)
CCTATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT47890.1136297307955315Clontech Universal Primer Mix Long (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGTTCCTATGTACTCTGCGTTGATA47240.11208746048822109Clontech SMARTer II A Oligonucleotide (95% over 24bp)
TGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGC45660.10833855727968193Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAA45330.10755555850827819Clontech Universal Primer Mix Long (96% over 25bp)
GAACAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG44430.1054201073135407Clontech SMARTer II A Oligonucleotide (100% over 22bp)
CATGTCAAGCAGTGGTATCAACGCAGAGTACATGTCAAGCAGTGGTATCA42680.10126784110155115Clontech Universal Primer Mix Long (96% over 26bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)