FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001834712

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001834712
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4214566
Sequences flagged as poor quality0
Sequence length151
%GC45

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA1070792.5406886497921732Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT988952.346504954484044Clontech Universal Primer Mix Long (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT877092.0810920982136714Clontech Universal Primer Mix Long (96% over 26bp)
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA492281.1680443490504124Clontech SMART CDS Primer II A (96% over 26bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC413750.9817143686918179Clontech Universal Primer Mix Long (96% over 25bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG327420.7768771446454985Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG281380.6676369524169273Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC216710.5141929204572903Clontech SMART CDS Primer II A (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG214810.5096847457128444Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT197710.46911117301283217Clontech Universal Primer Mix Long (96% over 26bp)
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC184170.43698449614978147Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG176660.41916534229147195Clontech SMART CDS Primer II A (96% over 26bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG171290.4064238168295383Clontech Universal Primer Mix Long (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC166940.3961024693883071Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC161330.3827914902744434Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT148010.3511868125923286Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA130660.3100200590048892Clontech Universal Primer Mix Long (96% over 26bp)
GCTTGCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT123530.2931025400954689Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTGCCATGTACTCTGCGT121780.2889502738834793Clontech Universal Primer Mix Long (96% over 28bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT119890.2844658263745306Clontech Universal Primer Mix Long (96% over 25bp)
ATGGCAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA110400.2619486798877987Clontech Universal Primer Mix Long (96% over 28bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTT109220.25914886609914284Clontech SMART CDS Primer II A (96% over 26bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC107530.2551389633001358Clontech Universal Primer Mix Long (96% over 26bp)
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG105940.25136633285609955Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT103820.2463361589307179Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC103090.2446040707394308Clontech Universal Primer Mix Long (96% over 26bp)
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA97280.23081854691562545Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA96790.22965591237626837Clontech SMART CDS Primer II A (96% over 26bp)
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA95150.22576464575474678Clontech Universal Primer Mix Long (96% over 25bp)
GTACATGGCAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA92310.2190261108735751Clontech Universal Primer Mix Long (96% over 28bp)
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC92040.2183854755151539Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGCAAGCAGTGGTA91720.21762620397924723Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATAGGAACAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT90800.21544329831351558Clontech SMARTer II A Oligonucleotide (100% over 22bp)
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT90660.21511111701655639Clontech Universal Primer Mix Long (96% over 26bp)
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA89150.21152830445649684Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGCAAGCAGTGGTATCAACGCAGAGTACATGGCAAGCAGTGGTA88730.21053176056561934Clontech Universal Primer Mix Long (96% over 28bp)
TCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA87600.20785058295444891Clontech Universal Primer Mix Long (96% over 26bp)
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG87270.2070675841830452Clontech SMARTer II A Oligonucleotide (100% over 23bp)
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA85440.20272550008707896Clontech Universal Primer Mix Long (96% over 26bp)
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC84240.19987823182742898Clontech Universal Primer Mix Long (96% over 26bp)
TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC83610.19838341599111273Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC79930.1896517933281861Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA79330.1882281591983611Clontech Universal Primer Mix Long (96% over 25bp)
GCTTGCCATGTACTCTGCGTTGATACCACTGCTTGCCATGTACTCTGCGT78850.18708925189450112Clontech Universal Primer Mix Long (96% over 28bp)
CCCATGTACTCTGCGTTGATACCACTGCTTGCCATGTACTCTGCGTTGAT77020.1827471677985349Clontech Universal Primer Mix Long (96% over 28bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT75500.17914062800297825Clontech Universal Primer Mix Long (100% over 23bp)
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT72780.1726868199477716Clontech Universal Primer Mix Long (96% over 26bp)
CATGGCAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA72410.17180891223437952Clontech Universal Primer Mix Long (100% over 26bp)
GCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT70110.1663516480700504Clontech Universal Primer Mix Long (96% over 26bp)
GTTCCTATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT66870.15866402376899544Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG66400.15754884370063252Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCCATGTACTCTGCGTTGATACCACTGCTTGCCATGTACTCTGCGTTGAT64900.15398975837607004Clontech Universal Primer Mix Long (96% over 28bp)
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT62100.14734613243688674Clontech Universal Primer Mix Long (96% over 26bp)
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC61740.14649195195899173Clontech Universal Primer Mix Long (96% over 25bp)
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC60490.14352604752185633Clontech SMART CDS Primer II A (96% over 26bp)
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC60170.14276677598594967Clontech Universal Primer Mix Long (96% over 26bp)
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA55580.1318759748927885Clontech Universal Primer Mix Long (96% over 26bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG54920.130309977349981Clontech Universal Primer Mix Long (96% over 26bp)
CATGTCAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA51780.12285962540389686Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATAGGAACAGTGGTAT51290.12169699086453979Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG50810.12055808356067979Clontech Universal Primer Mix Long (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG50800.12053435632518271Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA50750.12041572014769729Clontech SMART CDS Primer II A (96% over 26bp)
ACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGC49980.11858872301442189Clontech Universal Primer Mix Long (96% over 25bp)
TATCAACGCAGAGTACATGGCAAGCAGTGGTATCAACGCAGAGTACATGG49110.11652445352617566Clontech Universal Primer Mix Long (100% over 26bp)
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG47660.11308400437909857Clontech Universal Primer Mix Long (96% over 26bp)
ATGGCAAGCAGTGGTATCAACGCAGAGTACATGGCAAGCAGTGGTATCAA47420.1125145507271686Clontech Universal Primer Mix Long (96% over 28bp)
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG46260.10976219140950694Clontech Universal Primer Mix Long (96% over 25bp)
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG45430.10779283086324903Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCTATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT44940.10663019632389195Clontech Universal Primer Mix Long (96% over 26bp)
GAACAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG43740.10378292806424196Clontech SMARTer II A Oligonucleotide (100% over 22bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGTTCCTATGTACTCTGCGTT43550.10333211058979738Clontech SMARTer II A Oligonucleotide (95% over 24bp)
TGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGC43420.1030236565283353Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAA42790.10152884069201905Clontech Universal Primer Mix Long (96% over 25bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)