FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001835950

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001835950
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22503821
Sequences flagged as poor quality0
Sequence length6
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTGC4735842.1044603936371518No Hit
TCCAGA4683382.081148796908756No Hit
CTGTAT4612382.049598599277874No Hit
TATAAG3955591.7577414964329834No Hit
ATTCAT3783161.681118953088011No Hit
ATTGAA3728121.6566608843893667No Hit
TCGTGG3709041.6481823242372926No Hit
CTTAGC3497241.5540649741215058No Hit
ACTAAT3428321.5234390639705142No Hit
CCATGA3385591.5044511774244917No Hit
GCACAT3184621.4151463433698659No Hit
TGATAA3045621.3533790550502511No Hit
GGCCGT2974571.3218066389703331No Hit
GGTAAT2923691.2991971452314697No Hit
AGTTAG2712751.2054619524390993No Hit
GTGGTG2681571.191606527620354No Hit
GTCTCA2558621.1369713614412416No Hit
TAGAGC2504071.1127310335431482No Hit
CCTGCT2422721.0765816169618483No Hit
TCATGT2418221.0745819565486234No Hit
TGTGAT2380141.0576603857629332No Hit
TTCTAC2323811.0326290810791643No Hit
TTAGTA2215260.9843928282223716No Hit
AGGACT2180650.9690132177997683No Hit
TTACGT2153920.9571352349452121No Hit
GGTACC2079800.9241986060944939No Hit
CAGTCC2019650.8974698119043873No Hit
AAGCGC1991500.8849608250972135No Hit
GTCGAG1963640.8725807052944475No Hit
AAGAGG1888090.8390086288013043No Hit
TCAATG1887020.8385331539919376No Hit
ACTAGC1883800.83710228587403No Hit
CCTCGG1851370.8226913998293889No Hit
GAGAAG1845820.8202251519864115No Hit
TACGGC1779510.7907590448750903No Hit
CATGAG1752600.7788010756040052No Hit
AACTAG1745560.77567271797976No Hit
TTGAAT1710710.7601864590017846No Hit
CGTTGC1701560.7561204828282273No Hit
GACCAT1687140.7497126821262932No Hit
AACTCT1668120.7412607841130624No Hit
CCACTT1655300.7355639737802749No Hit
ATACCT1653550.7347863280640208No Hit
CCGTCG1633340.7258056309637372No Hit
GAGCAC1630370.7244858550910087No Hit
GCTTCT1551720.689536234757644No Hit
GACTTG1541630.685052551742213No Hit
CGTACT1531920.6807377289394543No Hit
CAAGGA1523390.6769472615339412No Hit
GCTGTC1494770.6642294213058307No Hit
AGATGC1474420.6551865125482468No Hit
CATAAC1462030.6496807808771675No Hit
ACCTGC1421430.6316394002600714No Hit
TACTCG1419390.6307328875394094No Hit
TTCTGT1396860.6207212544038632No Hit
CAGCCT1391070.6181483580055138No Hit
ACAGCC1387820.6167041588181846No Hit
TCTTCA1369240.6084477831564693No Hit
CGAAGC1345710.5979917810402064No Hit
CGTGAA1336520.5939080301074204No Hit
AACATA1318780.586024924389507No Hit
AAGGCG1312650.5833009425377139No Hit
AGTGCA1308380.5814034869900538No Hit
GCCATA1284150.5706364265872893No Hit
GTAAGA1274070.5661571872616655No Hit
ATCAGC1257680.5588739796677196No Hit
CTAGTT1256060.5581541019189585No Hit
CTGAGG1253050.5568165512870015No Hit
CACCTA1217220.5408948107079238No Hit
CAACGG1211350.538286364791117No Hit
GAAGAT1207070.5363844655536498No Hit
CGAATT1178190.5235510893905528No Hit
GAGTCG1158320.5147214777437129No Hit
CAAGCT1133830.5038388814059621No Hit
GACACG1125670.5002128305233142No Hit
CACATT1095970.48701507179602965No Hit
CCGGAA1091090.4848465511701324No Hit
TACAGT1067770.47448386653982005No Hit
GTACTT1066200.4737862072400949No Hit
GAGATA1054310.4685026600593739No Hit
GCGCAG1042990.46347240319766136No Hit
CCTTAT1014820.45095452901087335No Hit
TCGGAC991560.44061850651940393No Hit
AAGTTG989980.43991640352987166No Hit
CTATTA965070.42884717222021984No Hit
AGAGGT955200.42446125038054655No Hit
TTCCAT954410.42411019888578033No Hit
GCTCAA942700.41890663812158835No Hit
CTCCGC941330.4182978526180065No Hit
TACGAA938880.4172091486152507No Hit
ACCAGG937760.41671145535684806No Hit
TCCACG925560.41129015379210493No Hit
AGGCTT919240.4084817418339756No Hit
CTGTTC916070.40707309216510384No Hit
GAACCG898960.39946993890504195No Hit
CATACG896350.39831013586537145No Hit
GACCGA894920.3976746882229466No Hit
GGACTA893760.3971592202053153No Hit
CGCTTG893230.39692370464553556No Hit
AGGTGT880680.3913468739375416No Hit
CAGGAC854670.3797888367491014No Hit
CGAGCC824610.3664311051887588No Hit
GTTGGC795930.35368660282180525No Hit
CTTCAC787120.3497717121016915No Hit
ACACGA775740.344714793101136No Hit
AACCTT739390.3285619806520857No Hit
GCATAA737730.32782432814409607No Hit
GTTACA727770.3233984130961582No Hit
CTGGAG724420.3219097770107574No Hit
GAATAC697800.31008067474408013No Hit
GGCCAG689250.30628131995895275No Hit
TGTGTG684920.3043572022724496No Hit
TATGGT682260.30317518078374334No Hit
AATTGC676940.3008111378063308No Hit
ATGGCC676620.3006689397325014No Hit
CCAGCG662760.2945099856597686No Hit
GAGTGA658760.292732509736902No Hit
TGCATA651520.28951527831651347No Hit
CAGTAG647930.28791999367574067No Hit
TGACAC645730.286942381918164No Hit
ATGCCG643790.2860803060955737No Hit
ATGTGA634190.28181436388069386No Hit
ATTCGA628460.27926812962118747No Hit
AGTAGG627360.2787793237423991No Hit
GGAGGC624850.27766395760080037No Hit
ATTGCT612500.27217600068894965No Hit
TTAGCG610940.27148278507903173No Hit
TCTCTG609880.271011753959472No Hit
CGACAA602150.2675767817385323No Hit
TTCGTT597520.26551935335781424No Hit
GTACGC583680.2593692866646958No Hit
CATTAA580810.25809394769003896No Hit
CGTCTC566830.2518816693396202No Hit
CCTGAC566000.2515128430856253No Hit
CGTCAG561400.2494687457743287No Hit
GATTCA549740.24428740345917255No Hit
AATGTA546720.24294540913740825No Hit
TCTCGC543470.24150120995007915No Hit
ACGAAC535060.2377640668222521No Hit
GGCAGG530550.23575996271921998No Hit
TATTGG528320.23476901989222185No Hit
GACTAA524640.23313374204318457No Hit
GCCTGG522830.23232943418808744No Hit
ACGTAA519280.23075192430654334No Hit
CGAGAG490720.21806074621727572No Hit
CTACCG484390.21524789056933932No Hit
GAGCCA463870.2061294390850336No Hit
AGTTCC461900.2052540321930218No Hit
ATCCGG456420.20281889017869453No Hit
TGCGAC449410.1997038636238708No Hit
GATCCT438600.1949002349423238No Hit
CTCGTC429270.19075427235223744No Hit
ACGCTA426940.18971889262716762No Hit
AGTCAA414810.18432869689107462No Hit
AGAACG414270.18408873764148764No Hit
TGGCAT406840.1807870761147629No Hit
TCCTCC385190.1711664876822474No Hit
TTCACA379970.16884688160290648No Hit
AGCCTA353740.15719108323870867No Hit
TAAGTC353710.15717775216928714No Hit
GGATCG348600.15490702667782505No Hit
CCGCTC346350.15390719647121262No Hit
TATTCT340430.15127653210537No Hit
ATCCAC338500.15041889997258687No Hit
ACCGCT334910.1488236153318141No Hit
AAGCAT332330.14767714336156515No Hit
GCGTAT327640.14559305284200405No Hit
AACAAT324010.14397999344200257No Hit
TCAAGC322350.14324234093401295No Hit
TGCGGA306640.13626130424695432No Hit
AGACGG295770.1314310134265643No Hit
AATCGG291510.12953800156871137No Hit
TAATGA287770.1278760615808311No Hit
GGCGTT287710.1278493994419881No Hit
CCGACT277940.1235079145003864No Hit
GGATTC265770.11809994400506475No Hit
AGGTCA252260.11209651907558277No Hit
GCGGCT251030.11154994522930128No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)