FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001835954

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001835954
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19555168
Sequences flagged as poor quality0
Sequence length6
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAGCG2660421.3604690074766936No Hit
GTAAGA2410831.2328352280072459No Hit
GTCTCA2215071.1327286986233No Hit
GGTACC2201871.1259785648479217No Hit
GTTGGC2162121.105651457456157No Hit
AAGCGC2053481.0500958109897087No Hit
AGTTAG2005531.0255754386768756No Hit
CAGTCC1909430.9764324192970371No Hit
GCACAT1860590.9514569243281366No Hit
CCGTCG1853060.9476062798335457No Hit
ATTCAT1841980.9419402584523948No Hit
GAGATA1797930.9194142438459235No Hit
CTATTA1783990.9122856934801072No Hit
ACCAGG1775570.907979926329449No Hit
GAGCAC1735860.8876732738885189No Hit
GACTTG1687840.8631171054117254No Hit
CGTACT1685570.8619562869518687No Hit
GTCGAG1682870.8605755777705412No Hit
TGATAA1673980.856029464947578No Hit
CCGGAA1645560.8414962223796798No Hit
CCACTT1628440.8327415034225225No Hit
GTACTT1625620.8312994293886915No Hit
AGGACT1623620.8302766818469676No Hit
CAAGGA1606230.8213838919716773No Hit
CACATT1605100.8208060396106032No Hit
GAGAAG1584110.8100723041602097No Hit
TTACGT1573750.8047744718940794No Hit
CTTAGC1573660.8047284482547018No Hit
TTGAAT1569810.8027596592368831No Hit
GAATAC1536880.7859201209623973No Hit
CGAATT1519780.777175629480657No Hit
TGTGAT1510660.7725119006903954No Hit
TTCTAC1503770.7689885354091563No Hit
TACGGC1468890.7511518182814896No Hit
GCCATA1458050.7456085266053455No Hit
GCTCAA1456270.7446982812932111No Hit
TCTTCA1420300.7263041667553048No Hit
ATTGAA1414590.7233842225236827No Hit
CCTTAT1410800.7214461159321157No Hit
ATCCGG1410010.7210421306531348No Hit
AACATA1405130.7185466266513282No Hit
CTGTAT1397470.7146295035665252No Hit
TACAGT1393360.7125277573682823No Hit
AAGGCG1376590.7039520192309265No Hit
GCGCAG1364520.6977797378166222No Hit
TCATGT1351950.6913517695168868No Hit
CTGTTC1331930.6811140666242295No Hit
GGACTA1324690.6774117205231885No Hit
ACTAGC1321210.6756321398005888No Hit
AACTCT1311590.6707127241248962No Hit
GGTAAT1258770.6437019615479652No Hit
CAGTAG1249650.6390382327577038No Hit
CTGAGG1243810.6360518099358696No Hit
TCCAGA1236740.6324363973758753No Hit
AACTAG1222310.6250572738623366No Hit
CATGAG1196880.6120530388693157No Hit
ACTAAT1192210.6096649233593902No Hit
GACACG1165300.5959038551854936No Hit
CGAGCC1160360.5933776687574354No Hit
ACAGCC1153760.5900026018697461No Hit
TTAGTA1135110.5804654810431698No Hit
ACACGA1131770.5787574926484906No Hit
TCGGAC1117960.5716954208728864No Hit
TAGAGC1114480.5699158401502866No Hit
AACCTT1113580.5694556037565108No Hit
ATGCCG1108620.5669191898530354No Hit
CGTGAA1098600.561795224668998No Hit
TTCCAT1094410.5596525685690862No Hit
CTGGAG1081670.5531376667283042No Hit
TCAATG1076650.5505705703985769No Hit
GTGGTG1060340.5422300641958178No Hit
CCTCGG1051940.5379345245205769No Hit
ATTCGA1029740.5265820268074404No Hit
GACTAA1029000.5262036102170025No Hit
GCTGTC1028790.5260962217251215No Hit
GAAGAT1017650.5203995179177188No Hit
TGACAC1003790.5133118774535713No Hit
TACGAA1001520.5121510589937146No Hit
TGGCAT1000020.5113839983374215No Hit
CACCTA995030.5088322432208202No Hit
CGAAGC989190.5058458203989861No Hit
TCGTGG986860.5046543195128776No Hit
CATAAC984700.5035497521678157No Hit
ACCTGC980850.501580963149997No Hit
ATCCAC978250.5002513913457558No Hit
CCTGCT973930.4980422566556319No Hit
AATTGC965530.4937467169803911No Hit
ATGGCC959180.4904994935354174No Hit
TATGGT951890.4867715787458334No Hit
CAACGG946160.483841407038794No Hit
ACGAAC945860.48368799490753545No Hit
GTCTGC945690.4836010613664889No Hit
CATACG942550.4819953477259822No Hit
ATTGCT941540.48147886021741154No Hit
GTACGC936960.47913676834686364No Hit
TCCTCC931460.47632421260712254No Hit
ATACCT930850.4760122746068967No Hit
ACGCTA929110.4751224842455969No Hit
GGTGGT928950.47504066444225895No Hit
AAGAGG925680.4733684722115402No Hit
GGCCGT921520.4712411573247543No Hit
GAGTCG912110.4664291301409428No Hit
AGACGG901880.46119777646502447No Hit
TGCATA899130.459791498595154No Hit
TATTCT892350.4563243844287096No Hit
AGATGC888820.4545192350175667No Hit
AGTGCA887410.45379819800065124No Hit
CAGCCT874020.4469509032088091No Hit
TCAAGC867210.4434684478292388No Hit
GCGGCT865010.4423434255333424No Hit
AGTCAA856470.43797629353018086No Hit
AAGCAT854670.4370558207426292No Hit
CTTCAC850100.43471884260978993No Hit
GATTCA848540.43392109952724517No Hit
AGTAGG843540.43136423067293517No Hit
CAAGCT831950.42543740866864455No Hit
TATAAG831420.4251663805700877No Hit
GGATCG822900.4208094760423434No Hit
GCTTCT807100.4127297704627237No Hit
CGTCAG806730.4125405621675048No Hit
TTCACA805750.41203941587206006No Hit
GTTACA805220.41176838777350316No Hit
AGGCTT797440.4077898998361968No Hit
TAAGTC793670.405862020720047No Hit
CGCTTG785330.40159716347105784No Hit
GATCCT781500.3996386019286564No Hit
TAATGA780100.3989226786494496No Hit
GGAGGC762400.3898713629051921No Hit
AATCGG758730.3879946211661286No Hit
GAACCG757430.387329835264008No Hit
CGACAA755470.3863275426731184No Hit
ACGTAA750240.38365305785151016No Hit
AGGTGT743540.3802268535867347No Hit
AGCCTA739220.37801771889661084No Hit
AGTTCC735060.37589040400982493No Hit
CTCCGC732290.3744738986645372No Hit
GGATTC726080.37129826754748413No Hit
CCATGA722390.3694112983330033No Hit
CCGCTC721570.3689919718408965No Hit
CTCGTC720960.36868003384067066No Hit
CAGGAC720890.3686442376767103No Hit
TACTCG709950.36304980862348No Hit
CGAGAG704210.36011452317873205No Hit
GCATAA701850.3589076810794978No Hit
TGCGAC692930.3543462270434087No Hit
CCTGAC679510.34748359103844056No Hit
GCGTAT662140.33860102863856756No Hit
GAGCCA660490.33775726191664524No Hit
CTAGTT656890.33591631634154206No Hit
CTATGC634410.32442063397256415No Hit
CATTAA634390.3244104064971469No Hit
GACCGA630300.3223188877743213No Hit
ACCGCT624130.3191637116081028No Hit
AATGTA620320.31721537754111856No Hit
CCGACT616560.3152926121626774No Hit
GAGTGA614940.314464186653881No Hit
AACAAT610970.3124340327835588No Hit
TCCACG601160.30741745609140253No Hit
TTCTGT600560.3071106318288853No Hit
AGAACG599720.30668107786136123No Hit
GGCGTT581940.29758885221543485No Hit
ATCAGC578730.29594734241096776No Hit
TCTCGC578470.29581438523054365No Hit
TGCGGA559800.28626703692855004No Hit
TGTGTG553390.2829891310573246No Hit
TCTCTG546810.2796242916450526No Hit
TTAGCG539090.2756764861339979No Hit
ATGTGA536640.274423620395386No Hit
GGCAGG523960.2679394009808558No Hit
GACCAT492070.25163169142806646No Hit
CGTTGC490930.2510487253292838No Hit
AGAGGT487630.2493611918854392No Hit
CGTCTC484320.24766854470388597No Hit
CTACCG477460.2441605206357726No Hit
AAGTTG458000.23420918705479798No Hit
TATTGG455060.2327057481684637No Hit
TTCGTT404610.2069069414284756No Hit
GCCTGG400930.20502508595170338No Hit
AGGTCA381640.19516068591177532No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)