FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001835960

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001835960
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28271171
Sequences flagged as poor quality0
Sequence length6
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAGCG3852391.3626566794845534No Hit
GTAAGA3502501.238894561530543No Hit
GTCTCA3223341.1401508625164483No Hit
GTTGGC3142781.111655403308197No Hit
GGTACC3136651.1094871167522562No Hit
AAGCGC2977571.0532177814636685No Hit
AGTTAG2898351.0251963033296356No Hit
CAGTCC2750120.972764799873341No Hit
CCGTCG2688660.9510253395588036No Hit
GCACAT2646020.9359428373165017No Hit
ATTCAT2616970.9256673520881041No Hit
GAGATA2606930.9221160312036597No Hit
ACCAGG2577440.9116849103986531No Hit
CTATTA2524520.8929661951392109No Hit
GAGCAC2473300.8748487991530312No Hit
CGTACT2442200.863848193624523No Hit
GTCGAG2438080.8623908786799103No Hit
GACTTG2436850.861955806499844No Hit
CCGGAA2385860.8439197654741646No Hit
TGATAA2370430.8384619087762583No Hit
GTACTT2355890.833318860403766No Hit
CCACTT2341050.828069696865404No Hit
CAAGGA2326040.8227604013997157No Hit
CACATT2317540.8197538050334031No Hit
AGGACT2304700.8152120759341734No Hit
GAGAAG2298370.8129730459343195No Hit
TTGAAT2279790.8064009799947798No Hit
CTTAGC2271680.8035323333440982No Hit
TTACGT2266980.801869862412137No Hit
TGTGAT2188190.7740004826825179No Hit
CGAATT2185520.7730560577062762No Hit
GAATAC2174100.7690166070588303No Hit
TTCTAC2169430.7673647476434563No Hit
GCCATA2120790.7501599420837574No Hit
GCTCAA2119330.7496435149431907No Hit
TACGGC2103290.7439698907413492No Hit
TCTTCA2066390.7309177253393572No Hit
AACATA2044860.7233021936020974No Hit
TACAGT2021460.7150252106642487No Hit
ATTGAA2011420.7114738897798043No Hit
AAGGCG2008480.7104339611542798No Hit
ATCCGG1995130.7057118362730713No Hit
CCTTAT1978640.699879039322425No Hit
GCGCAG1971350.6973004407917875No Hit
CTGTAT1962910.6943150674586489No Hit
TCATGT1950140.6897980985647889No Hit
GGACTA1931370.6831588263535316No Hit
AACTCT1893870.6698944306197999No Hit
CTGTTC1882130.665741790462093No Hit
ACTAGC1874520.6630500024211944No Hit
GGTAAT1821650.6443489730227305No Hit
TCCAGA1801090.6370765469884498No Hit
CTGAGG1796430.6354282247452714No Hit
CAGTAG1772570.6269885318864225No Hit
AACTAG1726070.610540681176595No Hit
CATGAG1725670.6103991942887685No Hit
GACACG1705710.6033389985862276No Hit
ACTAAT1688120.5971171126940585No Hit
CGAGCC1640690.5803403049700346No Hit
ACAGCC1632120.5773089483983526No Hit
TCGGAC1628550.5760461779245013No Hit
TAGAGC1614620.5711188970559443No Hit
ACACGA1611750.5701037286357895No Hit
TTAGTA1591820.5630541444498355No Hit
CGTGAA1585980.5609884358875691No Hit
TTCCAT1580510.5590536026965419No Hit
CTGGAG1572780.5563193685892954No Hit
AACCTT1570070.555360794924271No Hit
ATGCCG1564940.5535462255878966No Hit
GTGGTG1544250.5462278163150723No Hit
TCAATG1519010.537299993693222No Hit
GACTAA1508640.5336319461263207No Hit
ATTCGA1504420.5321392594597514No Hit
GCTGTC1503270.5317324846572503No Hit
CCTCGG1484430.5250684522406235No Hit
TACGAA1463590.5176969853848643No Hit
TGACAC1448200.5122532773757408No Hit
GAAGAT1440670.5095897867124075No Hit
TGGCAT1432300.5066291735846387No Hit
CGAAGC1426840.5046978775658072No Hit
CATACG1421550.5028267134743022No Hit
ATCCAC1418020.5015780916892335No Hit
CACCTA1406240.497411302842744No Hit
AATTGC1404650.49684889246363373No Hit
TCGTGG1393700.49297568890938404No Hit
ACCTGC1390800.4919499089726421No Hit
CATAAC1389820.49160326609746724No Hit
CCTGCT1378230.4875036835226953No Hit
GTACGC1375720.48661585330158413No Hit
ATTGCT1369370.48436974895733886No Hit
ATGGCC1359000.4807017013904376No Hit
TCCTCC1353580.47878455406038894No Hit
TATGGT1352340.4783459447081269No Hit
GTCTGC1350970.4778613521173212No Hit
ACGCTA1349280.4772635700162543No Hit
CAACGG1345960.4760892288472946No Hit
ACGAAC1340170.4740412061460065No Hit
GGCCGT1332560.47134941810510783No Hit
ATACCT1329110.4701290936976046No Hit
GGTGGT1322680.46785469197579405No Hit
AAGAGG1302250.46062824918005696No Hit
GAGTCG1296090.45844935110752927No Hit
TATTCT1294280.4578091229401145No Hit
AGACGG1289070.45596625622617476No Hit
AGTGCA1287420.45538262281389047No Hit
TGCATA1281020.45311883260866703No Hit
GCGGCT1280920.45308346088671037No Hit
AGATGC1266780.44808189940204457No Hit
CAGCCT1260560.44588177829634296No Hit
AGTCAA1241330.4390797961640853No Hit
GATTCA1229190.43478566911855193No Hit
CTTCAC1227560.43420911005065904No Hit
AAGCAT1216280.4302191798139525No Hit
TCAAGC1215460.4299291316939083No Hit
AGTAGG1190600.42113572161549306No Hit
TATAAG1183450.41860664349559484No Hit
GGATCG1173810.4151968094989769No Hit
CGTCAG1169530.41368289979923367No Hit
CAAGCT1169110.4135343385670159No Hit
TTCACA1166870.4127420119951876No Hit
GTTACA1142500.4041219233543598No Hit
GCTTCT1140200.40330837374935763No Hit
AGGCTT1137490.4023498000843333No Hit
CGCTTG1136520.40200669438135406No Hit
GGAGGC1130230.3997818130702828No Hit
TAAGTC1124650.3978080709851035No Hit
GATCCT1115010.3943982369884856No Hit
TAATGA1111350.39310363196487336No Hit
AATCGG1105400.39099901450845453No Hit
GAACCG1102570.3899979947770823No Hit
CGACAA1096540.3878650799430982No Hit
AGGTGT1077710.381204584698667No Hit
ACGTAA1061610.37550973746365157No Hit
GGATTC1060580.3751454087274984No Hit
AGTTCC1059220.3746643533088884No Hit
AGCCTA1050710.3716542197703802No Hit
CTCCGC1050690.3716471454259889No Hit
CTCGTC1035020.36610439659538685No Hit
TACTCG1027630.36349042634279277No Hit
CCGCTC1026090.3629457018246609No Hit
CCATGA1022750.36176428631130986No Hit
CAGGAC1014600.35888149097184546No Hit
TGCGAC1012530.3581492963273435No Hit
GCATAA1007010.35619677727533816No Hit
CGAGAG1001510.3542513325677242No Hit
CCTGAC979900.3466075034528991No Hit
GCGTAT967140.34209407173123463No Hit
GAGCCA948680.33556445185804296No Hit
CTAGTT930510.3291374099785255No Hit
ACCGCT911460.3223990969457898No Hit
CTATGC901210.3187734954452364No Hit
GACCGA896160.3169872234864272No Hit
CATTAA891640.31538842165398806No Hit
AATGTA887010.3137507109273967No Hit
GAGTGA873110.3088340415754268No Hit
AACAAT867130.30671881260242106No Hit
CCGACT866500.30649597075409435No Hit
AGAACG866280.3064181529657898No Hit
TTCTGT855710.30267936195497525No Hit
TCCACG850280.3007586774527309No Hit
TCTCGC834810.29528667206604214No Hit
GGCGTT830630.29380813408825546No Hit
ATCAGC826820.29246047148170834No Hit
TGCGGA815030.28829014546302306No Hit
TCTCTG799400.28276154532120373No Hit
TGTGTG792250.2802324672013055No Hit
ATGTGA785180.2777316864589726No Hit
TTAGCG783620.2771798875964494No Hit
GGCAGG769050.27202622770737017No Hit
NNNNNN764060.27026117878173495No Hit
AGAGGT713250.25228880685557736No Hit
GACCAT706860.25002855382254946No Hit
CGTCTC699220.24732615426506388No Hit
CTACCG693370.24525690853060172No Hit
CGTTGC690180.2441285506001856No Hit
AAGTTG653710.23122848360260703No Hit
TATTGG651470.2304361570307788No Hit
GCCTGG587050.20764969374632555No Hit
TTCGTT584600.20678308655838842No Hit
AGGTCA562320.19890226690645393No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)