FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001835964

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001835964
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31197767
Sequences flagged as poor quality0
Sequence length6
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTACC6168071.9770870139519923No Hit
GAAGAT4528891.4516712045448636No Hit
ACTAGC4362621.3983757234932872No Hit
AGTTAG3951191.2664976951715807No Hit
ACACGA3860411.237399458749724No Hit
GCACAT3780471.2117758299816779No Hit
ACAGCC3716151.1911589698070377No Hit
CGAGCC3706651.188113880073532No Hit
CTGTAT3499241.1216315577970692No Hit
CCACTT3367201.0793080158589554No Hit
GCTGTC3341231.0709837021348356No Hit
CAGGAC3270541.0483250291599395No Hit
AACATA3206881.0279197225878378No Hit
TTACGT3205911.0276088028992587No Hit
CTATTA3190061.0225283110807257No Hit
CTTAGC3094260.9918209851365325No Hit
TGTGAT3033590.9723740804910813No Hit
GTCTGC3008710.9643991507469107No Hit
CAAGGA3008330.9642773471575706No Hit
TCATGT3006560.9637099988598543No Hit
GTAAGA2996680.9605431055370085No Hit
GCCATA2857590.9159597864808722No Hit
TCTTCA2853050.9145045541240179No Hit
TCCACG2832500.9079175442268032No Hit
GTCTCA2823630.9050743920229932No Hit
ACTAAT2784480.8925254169633359No Hit
TTGAAT2781280.8914997025267867No Hit
GAGATA2731950.875687673415857No Hit
CATACG2679010.8587185102061952No Hit
CGTACT2675860.857708822557717No Hit
ACGAAC2671730.8563850098630456No Hit
AAGCGC2637820.8455156421932378No Hit
CATGAG2625400.8415345880363809No Hit
GGACTA2524650.8092406100731505No Hit
GCGCAG2489600.7980058316353218No Hit
AACTCT2451490.785790213767543No Hit
CCGTCG2440720.782338043617032No Hit
CCAGCG2403060.7702666668418929No Hit
AACTAG2364780.7579965578946724No Hit
GAATAC2356750.7554226557304566No Hit
ATCCGG2331940.7474701634895856No Hit
TGATAA2311170.7408126357248581No Hit
CGAATT2277370.7299785269888066No Hit
CCTGCT2232530.7156057034466602No Hit
TGCATA2222180.712288158315946No Hit
CAGTCC2189960.7019604960829408No Hit
CTGTTC2189750.7018931835730422No Hit
CCTTAT2178460.698274334826592No Hit
TACGGC2176010.6974890222111089No Hit
GAGAAG2171600.6960754595032395No Hit
ACCAGG2110960.676638170930631No Hit
TCGTGG2097740.6724006881646369No Hit
TCCAGA2075100.6651437585260509No Hit
TAGAGC2071130.6638712315532069No Hit
GGCCGT2065290.6619993027065045No Hit
GGATCG2055270.6587875343770597No Hit
GTGGTG2040200.6539570604524356No Hit
GACTTG2029790.6506202831760363No Hit
TATTGG2013680.6454564520595336No Hit
GATCCT2011090.6446262644374516No Hit
CACATT1963880.6294937711407358No Hit
ATTGAA1956120.6270064136321039No Hit
CGAGAG1934650.6201245108343811No Hit
CCTCGG1922090.6160985816709253No Hit
AGGACT1887230.6049247050277669No Hit
TTCTAC1862740.5970747842305508No Hit
CAACGG1860220.5962670341117683No Hit
GTACTT1829740.5864971041036366No Hit
GGTAAT1823030.5843463091444974No Hit
TTAGTA1819800.5833109786351055No Hit
ATTCAT1812990.5811281300998241No Hit
CAGTAG1762210.5648513241348331No Hit
ATGCCG1750670.5611523414480273No Hit
CCATGA1749510.5607805199647782No Hit
AACCTT1738220.5571616712183279No Hit
TCAATG1729510.5543698047363455No Hit
GCTCAA1709430.5479334466469988No Hit
TACAGT1709030.5478052323424302No Hit
GAGCAC1678990.5381763380693241No Hit
GACACG1663420.5331855962639891No Hit
CTGGAG1622290.5200019603967169No Hit
GAGTCG1622020.519915415741133No Hit
ATGGCC1596160.5116263609507694No Hit
CGTGAA1583580.5075940210720851No Hit
GGCCAG1582950.5073920835423894No Hit
CGAAGC1542700.4944905191451683No Hit
AGACGG1538570.4931667064504969No Hit
GCTTCT1530970.49073063466369243No Hit
GAGCCA1508230.4834416514489643No Hit
GTCGAG1470360.47130296216392664No Hit
GTTGGC1457620.46721933656341497No Hit
CCGGAA1439920.4615458535862519No Hit
AAGGCG1432410.4591386300179753No Hit
CGTTGC1398760.44835260164613705No Hit
CGTCAG1345620.4313193312841909No Hit
CATAAC1341800.4300948846755603No Hit
TGTGTG1335350.42802742901439067No Hit
CAGCCT1298930.41635351658341446No Hit
CTGAGG1292930.41443030201488457No Hit
ACCTGC1269730.4069938723499025No Hit
AAGAGG1256780.40284293423949225No Hit
TCTCTG1238940.3971245762557301No Hit
AATCGG1207940.3871879676516592No Hit
GTACGC1188640.38100162745622146No Hit
AGCCTA1175970.3769404393590092No Hit
NNNNNN1159450.3716451885803237No Hit
GCATAA1135000.36380808921356456No Hit
ATTCGA1132530.3630163658828531No Hit
CCGACT1125610.3607982584138153No Hit
TCAAGC1116690.35793907942193426No Hit
GGCGTT1072270.3437008808995849No Hit
AGTTCC1061090.3401172910868909No Hit
CCTGAC1060250.33984804104729677No Hit
CAAGCT1045050.3349758974736878No Hit
ACGCTA1031750.33071277184677994No Hit
AGTGCA1016700.3258887086373842No Hit
AGATGC999280.32030497567341915No Hit
ATCCAC996490.31941068089905283No Hit
TACTCG992600.3181637967871226No Hit
TTCTGT987610.31656432333762863No Hit
CGTCTC982360.314881510590165No Hit
ATGTGA970480.3110735457444759No Hit
AGTCAA947960.3038550803972605No Hit
AACAAT941840.30189340153736005No Hit
GACCGA939830.3012491246569025No Hit
GGTGGT933040.29907268683684957No Hit
AAGTTG932810.29899896361172257No Hit
GGATTC931200.29848290103583375No Hit
GTTACA929030.29778733843354876No Hit
TATGGT925180.29655327575207546No Hit
ACCGCT920980.29520702555410455No Hit
AAGCAT918990.2945691593888755No Hit
TATTCT890750.2855172294863283No Hit
CTCGTC885510.2838376220964789No Hit
GAGTGA873660.28003927332363243No Hit
GCGGCT844730.2707661737457043No Hit
CACCTA837790.2685416555614381No Hit
AGGCTT827580.26526898543732313No Hit
CTTCAC826010.26476574429189115No Hit
TGCGAC816500.26171744920077133No Hit
TTCGTT806650.25856017195076814No Hit
GGAGGC800530.2565984930908677No Hit
CTAGTT799210.25617538588579114No Hit
ATCAGC793490.2543419213304593No Hit
CATTAA780140.2500627689154804No Hit
TTCCAT778460.24952426883629203No Hit
TGCGGA758490.24312316968070183No Hit
CCGCTC744550.23865490116648413No Hit
GCGTAT740920.23749135635252358No Hit
AGAGGT736750.2361547222273953No Hit
CTCCGC735320.23569635608856238No Hit
AGTAGG730820.23425394516216497No Hit
AGGTGT723930.23204545376596986No Hit
AATTGC722100.23145887332256826No Hit
TAAGTC719450.2306094535548009No Hit
GACTAA714620.22906126582713437No Hit
TGGCAT703310.2254360063654556No Hit
TTAGCG702360.225131497392105No Hit
CTATGC702030.2250257205908359No Hit
TCCTCC700740.224612229458602No Hit
TGACAC700190.22443593478982007No Hit
AGAACG683160.21897721077280946No Hit
TCGGAC672820.2156628709997097No Hit
ATACCT666410.21360823676899698No Hit
ATTGCT665120.21319474563676302No Hit
CTACCG664060.2128549777296561No Hit
CGACAA657880.21087406672407036No Hit
TATAAG654980.20994451301594758No Hit
GAACCG647930.20768473589792502No Hit
AATGTA644140.20646990536213697No Hit
TTCACA642860.20605961958751728No Hit
ACGTAA622310.1994726096903025No Hit
TACGAA612790.1964211092415685No Hit
TCTCGC598760.19192399250882283No Hit
CGCTTG596840.1913085638468933No Hit
GACCAT595100.1907508316220196No Hit
GGCAGG586110.18786921512683905No Hit
TAATGA584560.1873723846966355No Hit
GATTCA563780.18071165157429375No Hit
AGGTCA530220.16995447142098344No Hit
GCCTGG414290.1327947605993724No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)