FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001835966

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001835966
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22759811
Sequences flagged as poor quality0
Sequence length6
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTACC4527941.9894453429336476No Hit
GAAGAT3331961.4639664626389033No Hit
ACTAGC3141991.3804991614385551No Hit
AGTTAG2985281.311645338355402No Hit
ACACGA2809851.234566490908031No Hit
GCACAT2746731.2068333959363722No Hit
ACAGCC2718611.19447828455166No Hit
CGAGCC2715731.193212896187934No Hit
CTGTAT2557851.1238450090820176No Hit
CCACTT2453541.078014224283321No Hit
GCTGTC2439981.0720563540707784No Hit
CAGGAC2377961.0448065671547098No Hit
TTACGT2343871.029828411141024No Hit
CTATTA2340961.0285498416485093No Hit
AACATA2332031.0246262589790398No Hit
CTTAGC2251550.9892656841482559No Hit
TGTGAT2232100.98071991898351No Hit
GTAAGA2193760.9638744363914095No Hit
CAAGGA2189540.9620202909417832No Hit
TCATGT2185520.9602540196840826No Hit
GTCTGC2176510.956295287337843No Hit
TCTTCA2102990.9239927343860633No Hit
TCCACG2097210.921453170239419No Hit
GTCTCA2089980.9182765182013155No Hit
GCCATA2085470.9162949551733974No Hit
TTGAAT2054790.90281505413204No Hit
ACTAAT2019120.8871426920021436No Hit
GAGATA2005120.880991498567365No Hit
CGTACT1979530.8697479957105092No Hit
CATACG1955640.8592514234850193No Hit
CATGAG1943000.8536977745553336No Hit
ACGAAC1939350.8520940705526949No Hit
AAGCGC1889100.8300156798314362No Hit
GGACTA1848580.8122123685473487No Hit
GCGCAG1843330.8099056710093067No Hit
CCGTCG1804790.7929723142252807No Hit
CCAGCG1780370.7822428753911883No Hit
AACTCT1776430.780511753810258No Hit
AACTAG1747750.7679105946881545No Hit
ATCCGG1718480.7550502066998711No Hit
TGATAA1689870.7424798035449416No Hit
GAATAC1685790.7406871700296632No Hit
CGAATT1645970.7231914184173146No Hit
CCTGCT1632300.717185217399213No Hit
TGCATA1621270.7123389557145268No Hit
CTGTTC1615780.7099268091461743No Hit
TACGGC1614050.7091666973860196No Hit
GAGAAG1607480.70628003018127No Hit
CCTTAT1605270.7053090203604941No Hit
CAGTCC1588690.6980242498498779No Hit
TCGTGG1549580.6808404516188645No Hit
ACCAGG1546030.6792806847121885No Hit
GGATCG1542450.6777077366767238No Hit
TAGAGC1535710.6747463764088375No Hit
GGCCGT1525520.6702691863302379No Hit
GTGGTG1511650.664175111120211No Hit
TCCAGA1509660.6633007629105532No Hit
TATTGG1505820.6616135784255853No Hit
GACTTG1501930.6599044253926362No Hit
GATCCT1474110.647681125295812No Hit
ATTGAA1457350.6403172680124629No Hit
CGAGAG1425820.6264639016554224No Hit
CACATT1423200.6253127497411995No Hit
TTAGTA1423170.6252995686124108No Hit
CCTCGG1419790.6238144947688713No Hit
AGGACT1401360.615716887982945No Hit
CAACGG1375620.6044074794821451No Hit
TTCTAC1362540.5986605073302234No Hit
GTACTT1345830.5913186185948556No Hit
GGTAAT1341990.5896314341098878No Hit
ATTCAT1329890.5843150454984006No Hit
ATGCCG1292300.5677990911260203No Hit
CAGTAG1289850.5667226322749341No Hit
CCATGA1286860.5654089131056492No Hit
AACCTT1273030.5593324127340074No Hit
TCAATG1266170.5563183279509659No Hit
TACAGT1255280.5515335782006274No Hit
GCTCAA1255000.5514105543319319No Hit
GACACG1220660.5363225555783393No Hit
GAGTCG1213940.5333699827296456No Hit
GAGCAC1209180.531278576961821No Hit
CTGGAG1185810.5210104776353371No Hit
ATGGCC1161070.5101404400941643No Hit
GGCCAG1159620.509503352202705No Hit
CGTGAA1153330.506739708866651No Hit
CGAAGC1128930.49601905745175123No Hit
AGACGG1120530.49232834139088416No Hit
GCTTCT1111000.4881411361456384No Hit
GAGCCA1110970.48812795501684964No Hit
GTTGGC1091220.4794503785642157No Hit
GTCGAG1080220.4746172980083183No Hit
AAGGCG1061570.466423029611274No Hit
CCGGAA1052050.4622402180756246No Hit
CGTTGC1011280.4443270640516303No Hit
CGTCAG991880.4358032674348658No Hit
TGTGTG991330.4355616134070709No Hit
CATAAC973790.4278550467752127No Hit
CAGCCT944480.41497708394854416No Hit
CTGAGG938220.41222662174127894No Hit
AAGAGG937070.41172134513770786No Hit
ACCTGC929730.4084963622940454No Hit
TCTCTG904600.39745497007861796No Hit
AATCGG885930.38925191426238115No Hit
GTACGC857850.37691437771605396No Hit
AGCCTA848070.3726173297309015No Hit
ATTCGA838540.3684301244856559No Hit
GCATAA829630.36451532923537894No Hit
CCGACT817270.35908470417438876No Hit
TCAAGC813640.3574897875909427No Hit
AGTTCC792180.3480608867973464No Hit
CCTGAC782630.3438648941329082No Hit
GGCGTT782080.34362324010511336No Hit
CAAGCT758800.33339468416499596No Hit
ACGCTA753550.331087986626954No Hit
AGTGCA752260.3305211980890351No Hit
AGATGC732490.32183483421720854No Hit
TACTCG730990.321175777777768No Hit
TTCTGT727360.3195808611943219No Hit
ATCCAC724150.31817048041391904No Hit
CGTCTC714310.313847070171189No Hit
ATGTGA713950.3136888966257233No Hit
GGATTC700080.3075948214156963No Hit
GGTGGT695420.3055473527438343No Hit
AAGTTG694940.3053364546832133No Hit
AGTCAA693720.3048004221124683No Hit
GTTACA690950.30358336455430146No Hit
TATGGT685710.30128106072585575No Hit
GACCGA683390.30026172009952107No Hit
AACAAT676080.2970499183846474No Hit
ACCGCT671280.2949409377784376No Hit
AAGCAT667090.29309997345760036No Hit
TATTCT660370.29014740060890665No Hit
GAGTGA649420.2853362886009906No Hit
CTCGTC646040.28385121475745123No Hit
CACCTA625870.2749891025017739No Hit
CTTCAC610460.26821839601392117No Hit
GCGGCT609470.26778341876389045No Hit
AGGCTT607250.26680801523351844No Hit
TGCGAC593040.2605645538972182No Hit
TTCGTT592820.26046789228610023No Hit
GGAGGC592770.2604459237381189No Hit
CTAGTT580260.25494939303318465No Hit
ATCAGC575700.2529458614572854No Hit
TTCCAT574360.25235710437138514No Hit
CATTAA568000.2495627050681572No Hit
TGCGGA558580.2454238306284705No Hit
AGTAGG551230.24219445407521176No Hit
CCGCTC547960.2407577110372314No Hit
AGAGGT544250.23912764477701506No Hit
GCGTAT542520.2383675330168603No Hit
CTCCGC540480.2374712162592211No Hit
TTAGCG539680.2371197194915195No Hit
AGGTGT537260.23605644176922205No Hit
TAAGTC535630.23534026710503No Hit
AATTGC523450.22998872881677268No Hit
GACTAA523400.22996676026879134No Hit
TCCTCC523010.22979540559453676No Hit
TGACAC516600.2269790377433275No Hit
TGGCAT512000.22495793132904313No Hit
CTATGC511180.22459764714214894No Hit
AGAACG509680.22393859070270836No Hit
ATTGCT495600.2177522475911597No Hit
TCGGAC494830.21741393195224687No Hit
CTACCG490990.21572674746727907No Hit
TATAAG485860.21347277444439236No Hit
CGACAA484520.2128840173584921No Hit
ATACCT482650.21206239366398957No Hit
GAACCG481970.2117636214114432No Hit
TTCACA472480.2075939910045826No Hit
AATGTA471980.20737430552476907No Hit
ACGTAA460650.20239623255219474No Hit
TACGAA446300.19609125928154678No Hit
TCTCGC436260.1916799748468913No Hit
CGCTTG435130.19118348566251275No Hit
GGCAGG433300.19037943680639527No Hit
TAATGA430440.18912283586186193No Hit
GACCAT429830.18885481957648947No Hit
GATTCA418040.18367463596248668No Hit
AGGTCA397190.17451375145426296No Hit
GCCTGG303220.13322606237811024No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)