FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001835974

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001835974
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24851050
Sequences flagged as poor quality0
Sequence length6
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTGA3457871.391438188728444No Hit
GAACCG3072901.2365272292317628No Hit
GATTCA3051421.227883731270912No Hit
TAAGTC3004221.2088905700161563No Hit
TTCCAT2996691.205860516960048No Hit
AGTGCA2862381.151814510855678No Hit
CCGACT2786731.1213731411751213No Hit
TATAAG2737721.1016516404739436No Hit
ATCAGC2649631.066204446089803No Hit
TTCTGT2625001.056293396053688No Hit
ATACCT2525161.0161180312300688No Hit
TACGAA2483490.9993501280629993No Hit
TAATGA2455450.9880669026057248No Hit
AAGCAT2424100.9754517414757123No Hit
TACTCG2414490.9715847016524454No Hit
TGCGAC2405830.9680999394391786No Hit
CGACAA2396180.9642168037165432No Hit
TGACAC2386420.9602894042706445No Hit
AATTGC2343340.9429541206508377No Hit
GGCGTT2338730.9410990682486252No Hit
AGAACG2334700.9394774063872553No Hit
TTCACA2290210.9215747423147111No Hit
AGCCTA2256120.9078570120779604No Hit
AGTTCC2221650.8939863707972097No Hit
CGTCTC2202620.8863287466726759No Hit
GGTGGT2193980.8828520324090934No Hit
GACCAT2191330.8817856790759344No Hit
ATTCGA2182450.8782123894161414No Hit
TCGGAC2129600.8569456823755938No Hit
CTAGTT2114140.8507246172696927No Hit
TCAAGC2089620.8408578309568409No Hit
TTACGT2045360.8230477183056651No Hit
CTTCAC2034480.8186696336774503No Hit
ATTGCT2025090.8148911213007096No Hit
CTCCGC1964460.7904937618330009No Hit
AACAAT1949930.7846469263874162No Hit
AGGTGT1945460.7828482096329933No Hit
GGCAGG1933860.7781803988161466No Hit
CCTGAC1911540.7691988869685586No Hit
AATCGG1906510.7671748276229777No Hit
AGGTCA1895260.7626478559256048No Hit
CTACCG1893960.7621247391961306No Hit
GCGTAT1878440.7558795302411769No Hit
TGGCAT1870910.7528494771850687No Hit
TTAGCG1858440.7478315805569583No Hit
GAGCCA1775680.7145291647636619No Hit
CTATGC1736750.6988638307033305No Hit
AGAGGT1711150.6885624551075307No Hit
AGGCTT1702580.685113908667843No Hit
CATAAC1678860.6755690403423598No Hit
TCTCGC1665350.6701326503306702No Hit
TATTCT1636290.6584389794395005No Hit
CACCTA1624010.6534975383333903No Hit
ACCGCT1611810.6485882890260171No Hit
AGTCAA1611110.6483066107870693No Hit
CGCTTG1580140.6358443607010569No Hit
TCAATG1539760.6195955502886196No Hit
GGATTC1517870.6107870693592423No Hit
GACTAA1515440.6098092434726098No Hit
ACCTGC1512050.6084451160011347No Hit
CTATTA1506550.6062319298379746No Hit
AGATGC1489750.5994716521032311No Hit
TCCTCC1483810.5970814110470182No Hit
CAAGCT1475090.5935725049846988No Hit
GTTACA1466810.5902406538154323No Hit
TATTGG1438970.5790379078550001No Hit
CGTCAG1416940.5701730912778333No Hit
ATCCAC1416690.5700724919067807No Hit
AGACGG1413220.5686761726365687No Hit
TATGGT1379270.5550147780476076No Hit
GATCCT1357020.5460614340239145No Hit
ACTAGC1347370.5421782983012791No Hit
GCATAA1344870.5411723045907517No Hit
AAGTTG1329870.5351363423275878No Hit
GGATCG1310600.5273821428068431No Hit
CGAGAG1291180.5195675836634669No Hit
GACCGA1286330.5176159558650439No Hit
AGTAGG1269600.5108838459541951No Hit
GCCTGG1267170.5099060200675626No Hit
TGCGGA1251960.5037855543327143No Hit
GTCTCA1242430.4999507063081841No Hit
CCGCTC1238960.4985543870379723No Hit
CTTAGC1235830.497294882912392No Hit
GCACAT1190330.47898579738079483No Hit
ACGTAA1184750.4767404194188978No Hit
GAATAC1178090.474060452174053No Hit
CATTAA1175570.47304641051384144No Hit
GGAGGC1162130.4676381883260466No Hit
GTACGC1139940.45870898815140604No Hit
TAGAGC1099510.4424400578647582No Hit
ACGCTA1093990.44021882375191396No Hit
AACCTT1093120.43986873794065035No Hit
AATGTA1078400.4339454469730655No Hit
TTGAAT1073550.43199381917464247No Hit
GCGGCT1053670.42399415718852923No Hit
ACTAAT1048530.4219258341196851No Hit
GTAAGA1039430.4182640170133656No Hit
GGTACC1024570.4122843903979912No Hit
GAGTGA1021460.41103293422209525No Hit
GACACG1009330.4061518527386167No Hit
ATCCGG1006500.40501306785829977No Hit
GGCCGT997130.40124260343124335No Hit
TGATAA991150.398836266475662No Hit
GAGCAC983080.3955889187780798No Hit
GGTAAT982180.39522676104228993No Hit
TCTCTG973760.39183857422523394No Hit
GCTTCT970670.39059516599902216No Hit
CCACTT958960.3858830914589122No Hit
TACGGC947390.38122735256659174No Hit
AAGGCG945550.38048694119564364No Hit
GGACTA929420.37399626977532135No Hit
GCGCAG924590.3720526899265826No Hit
AAGCGC922700.3712921586814239No Hit
GAGATA920600.37044712396458096No Hit
CTGTTC914740.3680890747071049No Hit
TGTGTG913460.367574005927315No Hit
TACAGT913060.3674130469336306No Hit
TTCGTT908080.36540910746226013No Hit
GCTGTC904670.3640369320411009No Hit
TCCAGA904470.36395645254425874No Hit
ACGAAC898260.36145756416730884No Hit
CAGTAG897420.36111955028057163No Hit
CGTACT893750.35964275151351754No Hit
TCATGT882990.35531295458340795No Hit
CCTGCT882710.3552002832878289No Hit
TCTTCA880990.3545081596149861No Hit
CGAGCC865770.34838366990529573No Hit
CAGCCT862970.34725695694950515No Hit
GAAGAT856130.3445045581575024No Hit
TGTGAT852950.34322493415771166No Hit
TTCTAC850340.3421746767239211No Hit
CGTGAA844630.3398769870890767No Hit
CATGAG843870.3395711650010764No Hit
GTACTT842930.33919291136591817No Hit
AACTAG838820.3375390577058112No Hit
CCAGCG837030.3368187662090737No Hit
AGGACT836990.3368026703097052No Hit
CAGTCC835090.33603811508970444No Hit
GAGAAG831810.33471825134149263No Hit
TGCATA823100.3312133692540154No Hit
ATGCCG820720.33025566324159344No Hit
CTCGTC814150.3276119117703276No Hit
CGAATT810170.3260103697831681No Hit
GCTCAA795940.32028425358284657No Hit
ACACGA793280.31921387627484554No Hit
CATACG784640.3157371620112631No Hit
ACAGCC781300.3143931544139986No Hit
CTGTAT775560.3120833928546279No Hit
TTAGTA774150.3115160124018905No Hit
GTCTGC773040.3110693511944163No Hit
AACTCT764110.30747594166041275No Hit
TCGTGG754640.30366523748493524No Hit
ACCAGG753840.3033433194975665No Hit
CGTTGC751840.30253852452914465No Hit
CAGGAC751220.30228903808893387No Hit
CCTTAT747120.30063920840366903No Hit
CCATGA746420.30035753016472144No Hit
CCTCGG742390.29873586830335136No Hit
CCGTCG727940.29292122465650344No Hit
AGTTAG726050.29216069341134476No Hit
ATGGCC716660.28838218103460417No Hit
AACATA715920.2880844068962881No Hit
ATTGAA704980.2836821784190205No Hit
GTGGTG684460.27542498204301225No Hit
CACATT676220.2721092267731142No Hit
GGCCAG671380.27016162294953333No Hit
ATTCAT669650.26946547530184844No Hit
CTGGAG659580.2654133326358444No Hit
CAACGG654050.26318807454815796No Hit
AAGAGG625380.2516513386758306No Hit
GTTGGC619460.24926914556930188No Hit
GACTTG616150.24793720989656373No Hit
CAAGGA614020.24708010325519444No Hit
TCCACG608550.2448789890165607No Hit
GCCATA605060.24347462179666454No Hit
CTGAGG591230.23790946459002738No Hit
GTCGAG590500.23761571442655344No Hit
GAGTCG552310.22224815450453805No Hit
CCGGAA550010.2213226402908529No Hit
CGAAGC514100.20687254663283844No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)