FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001835978

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001835978
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22732728
Sequences flagged as poor quality0
Sequence length6
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATAC9248084.068178706928618No Hit
CCTTAT8196993.605810090192431No Hit
ATGGCC7938973.4923085341979196No Hit
GGCCAG7016573.086549929247383No Hit
CAGTAG6544042.878686623092486No Hit
ATGCCG6508772.863171547207181No Hit
GGCTAC3699391.6273409860884274No Hit
ACTAAT3251521.4303254761153172No Hit
ATTGAA3246241.4280028336238397No Hit
CGAATT3225261.4187738488755068No Hit
GTAAGA3195571.4057133838050584No Hit
TGATAA3160981.3904974361194133No Hit
ACTTGA3112371.3691141687878374No Hit
CTGGAG3094691.3613368355966782No Hit
ATTCAT2929101.2884947200353605No Hit
CACATT2926121.2871838346897917No Hit
GATCAG2861421.2587226662809672No Hit
GAGATA2727491.1998076077802893No Hit
GTCTGC2670171.174592860126598No Hit
CTGTAT2555371.1240929817134133No Hit
TAGCTT2510401.104310930038841No Hit
GGACTA2504031.101508802639085No Hit
CAGATC2431891.0697748198104513No Hit
CGAAGC2309941.0161296963567241No Hit
AGCCTA2191750.9641385758893521No Hit
GGCGTT2104350.9256918043448196No Hit
CCGTCG1884960.8291833694574624No Hit
AGACGG1868630.8219998937215102No Hit
GAGAAG1863410.8197036448947086No Hit
TTACGT1823800.802279427264515No Hit
GCCATA1745760.7679500674094196No Hit
AACAAT1727540.7599351912361771No Hit
AAGGCG1693020.7447500361593206No Hit
GGTGGT1667960.7337262822130278No Hit
CCGACT1595630.7019087194462539No Hit
GAAGAT1522120.6695720812741877No Hit
CAGCCT1500440.6600351704379694No Hit
CAGTCC1480020.6510525265599447No Hit
GACTTG1467220.6454218780957569No Hit
TCAATG1388940.6109869435819582No Hit
TAGAGC1375260.6049691880358574No Hit
AGTTAG1372460.6037374836843163No Hit
GGTAAT1353550.5954190803673013No Hit
CGTGAA1351280.5944205200537305No Hit
GCTTCT1339350.5891725797273429No Hit
CTTAGC1325640.5831416273489042No Hit
TTGAAT1321270.5812192887716776No Hit
AGGACT1290460.5676661419606129No Hit
GGTACC1288500.5668039489145341No Hit
CAGGAC1226580.5395656869690254No Hit
CCTGCT1225100.5389146432403538No Hit
ACTAGC1219240.5363368619903428No Hit
GCTCAA1181410.5196956564122001No Hit
TCGTGG1176930.5177249294497344No Hit
ACACGA1167810.5137130924190005No Hit
CGTTGC1166010.512921282478724No Hit
CGTACT1143650.5030852434428459No Hit
TCCAGA1133300.49853233628625654No Hit
GCGCAG1118470.4920087021672014No Hit
GTCTCA1115810.49083858303323735No Hit
TTCTAC1114650.4903283055161704No Hit
ATCCGG1107890.48735461929602114No Hit
CTGTTC1099360.48360231996793346No Hit
CCACTT1074200.47253457658051423No Hit
CATACG1064010.46805205252972715No Hit
GTACTT1046470.46033630455614477No Hit
CGAGCC1022990.4500075837796502No Hit
GAGCAC1016630.4472098553240069No Hit
AACCTT992360.4365336179626132No Hit
TACAGT982740.43230183372624703No Hit
AACTCT964200.4241461913413999No Hit
TTAGTA955620.42037189729274904No Hit
CAAGGA949070.4174905888989654No Hit
TCTTCA947660.4168703377790822No Hit
TGTGAT944000.4152603242338535No Hit
TGACCA943410.41500078653120737No Hit
AATCGG942110.4144289237965632No Hit
TACGGC933930.4108305875124182No Hit
TCATGT924860.4068407452022476No Hit
AAGCGC920330.4048480235192186No Hit
GTGGTG905890.3984959482205567No Hit
CCATGA898000.39502518131567843No Hit
CCAGCG890840.39187553733102337No Hit
ACCAGG890810.39186234049868546No Hit
GCACAT888510.3908505833527766No Hit
ACAGCC880760.38744140166547547No Hit
GCTGTC871680.3834471604111922No Hit
GCCAAT861390.3789206469192787No Hit
CGATGT858900.37782530983522966No Hit
AACTAG858630.37770653834418816No Hit
TGCATA851340.3744997080860687No Hit
CCTCGG849220.37356713193418756No Hit
GTCGAG838170.36870629868971294No Hit
GAGTCG828210.36432495035351675No Hit
ACGAAC826810.36370909817774616No Hit
GGATTC817190.35947731394138005No Hit
TCCACG809040.35589217448957294No Hit
CTGAGG803680.3535343404451943No Hit
GGCCGT792810.34875268819474725No Hit
ACAGTG789600.34734062713458763No Hit
AACATA783290.3445648933995075No Hit
ACCGCT768800.3381908233802824No Hit
TTAGGC764380.3362464900824925No Hit
CTAGTT763800.335991351323959No Hit
TATAAG736580.32401742544933454No Hit
TATTCT727430.3199923915862628No Hit
AAGAGG722220.3177005417035738No Hit
AGTCAA711650.3130508577765062No Hit
CCGGAA691730.30428816110411383No Hit
GACCAT687340.30235702463866193No Hit
CAACGG686850.3021414763771422No Hit
ATCCAC683040.30046547867022383No Hit
TTCTGT615150.27060104708946503No Hit
GACACG604300.2658281927272433No Hit
ATACCT590680.25983683084581843No Hit
ATCAGC585110.2573866189750742No Hit
CATGAG582550.2562604892822366No Hit
CTACCG571500.25139965603776193No Hit
AGTTCC504360.22186514526545165No Hit
TCTCTG496060.21821402165195483No Hit
CTATTA452000.19883227389163324No Hit
AGTGCA447880.1970199089172228No Hit
TTCCAT440140.1936151261740342No Hit
GTTGGC414130.18217347253703997No Hit
TAAGTC410000.18035670861851688No Hit
TAATGA399560.17576421096491368No Hit
TGGCAT392250.17254858281856889No Hit
TGTGTG380130.16721706255404103No Hit
TACGAA375090.16499999472126706No Hit
AAGCAT369550.16256297968286076No Hit
TACTCG364800.16047348122935357No Hit
GAACCG355300.15629448432233914No Hit
TCAAGC350060.15398943760731226No Hit
AATGTA343070.15091457567257216No Hit
CTTCAC330770.14550387441401663No Hit
GACCGA330150.14523113987903255No Hit
TCGGAC323570.142336634652911No Hit
CATTAA318540.14012396576424968No Hit
AGGTCA313680.13798607892550335No Hit
CGTCAG313510.13791129687558834No Hit
CTCCGC311660.1370974922147487No Hit
AAGTTG311110.13685555028855315No Hit
CTATGC305280.13429096587088007No Hit
CATAAC300010.13197272232351526No Hit
CGTCTC298370.1312512954890412No Hit
CGACAA297430.13083779474245238No Hit
CGAGAG287490.12646524429448153No Hit
TTAGCG285840.1257394185158948No Hit
GACTAA284450.1251279652842369No Hit
AGGTGT283130.12454730466136751No Hit
ACGCTA280550.12341237708030466No Hit
GCATAA274160.12060145179232339No Hit
TTCGTT270420.1189562466941935No Hit
AGAGGT264560.11637846544418251No Hit
GAGCCA257540.11329040667710448No Hit
AGTAGG255480.11238422418989925No Hit
GATCCT254270.11185195195226899No Hit
TCTCGC252150.1109193758003879No Hit
CTCGTC251710.11072582225943141No Hit
AATTGC250200.11006158169842176No Hit
ATGTGA248590.10935335169628564No Hit
AGGCTT245160.10784451386564779No Hit
CCTGAC244070.10736502895736931No Hit
ATTCGA243890.10728584796334166No Hit
GGAGGC242560.10670078839635963No Hit
GTTACA239480.10534591360966444No Hit
TGCGAC236890.10420658708448893No Hit
TTCACA232900.10245140838354289No Hit
ATTGCT231440.10180916254309647No Hit
GTACGC231420.10180036465487116No Hit
TGACAC227930.10026513315955744No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)