FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001835982

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001835982
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24862392
Sequences flagged as poor quality0
Sequence length6
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCGTT10172644.091577351044903No Hit
TGATAA7447542.9955042137538497No Hit
ATTCAT6043472.4307677233952387No Hit
CTGTAT5797662.33189952117238No Hit
ACTAAT5460492.1962850557581106No Hit
GGCTAC5375392.162056651668914No Hit
CCGACT5050942.0315583472418903No Hit
ACTTGA4284651.7233458470126286No Hit
ATTGAA4139761.665069072999895No Hit
GTCTGC4009891.612833551976817No Hit
CAGATC3916121.5751179532524466No Hit
TAGCTT3615611.454248649928776No Hit
TTCTGT3510421.4119397683054793No Hit
TATAAG3384021.3610999295642994No Hit
CGTACT3319701.3352295306099267No Hit
GATCAG3211451.2916898744095098No Hit
ATCAGC3049261.226454799682991No Hit
AGCCTA2738741.1015593350792634No Hit
CTAGTT2511971.0103492857807084No Hit
TCTTCA2453500.9868318382237719No Hit
CTCGTC2362310.9501539513977577No Hit
ACGCTA2358590.9486577156373368No Hit
TCAAGC2208510.8882934514104676No Hit
TGACCA2178890.8763798752750742No Hit
ACAGTG2166180.8712677364269696No Hit
CGATGT2129470.8565024636406666No Hit
GTACGC2124510.8545074826267722No Hit
GCGCAG2110400.8488322442989394No Hit
TTAGGC2106310.8471871893903048No Hit
CCAGCG2101350.8451922083764104No Hit
GACCAT1968130.7916092707411257No Hit
ACGTAA1967410.7913196767229798No Hit
ATACCT1940410.7804599010425063No Hit
AAGCAT1926510.7748691276366328No Hit
GGTGGT1840750.7403752623641361No Hit
TGTGAT1812650.7290730513781618No Hit
CCGGAA1752070.7047069324624919No Hit
GCTCAA1698420.6831281559714769No Hit
ATGCCG1685560.6779556850362588No Hit
GAATAC1677370.6746615530798485No Hit
GGTACC1675950.6740904093218384No Hit
CCTTAT1639820.6595584206057085No Hit
CTATTA1615450.6497564675192958No Hit
CAGTAG1548770.622936843727667No Hit
GCCAAT1538040.6186210884294641No Hit
ATGTGA1518840.6108985812789052No Hit
TATTGG1496780.6020257423340442No Hit
TGCGAC1488770.5988040088821703No Hit
GAGCCA1420230.5712362672103312No Hit
ATCCAC1410430.5672945708522333No Hit
AGGCTT1391770.5597892592152839No Hit
GTGGTG1341270.5394774565536574No Hit
TTCTAC1338020.5381702613328596No Hit
GTCTCA1318970.5305080862694145No Hit
ATGGCC1286220.5173355805829142No Hit
GATCCT1248570.502192226717365No Hit
AGGTCA1206810.48539577366489917No Hit
TAAGTC1165310.46870389623009723No Hit
GGCCAG1151490.46314529993735115No Hit
CCGTCG1140750.45882552250000724No Hit
GAGTGA1098580.44186416174276394No Hit
AGATGC1095410.4405891436350935No Hit
AGTCAA1088880.4379626867760753No Hit
AATGTA1042100.4191471198748697No Hit
CTACCG1036780.41700734185190225No Hit
AGACGG1017900.4094135431538526No Hit
CAGTCC995170.40027122088655026No Hit
TCATGT976760.39286646272812364No Hit
CCACTT974340.391893105056022No Hit
AGTTAG930560.3742841798970911No Hit
TTACGT922380.37099407007982177No Hit
AACAAT919070.36966274202417854No Hit
GTTACA906760.3647114887417108No Hit
CTTAGC903690.3634766920254495No Hit
CAGGAC869350.34966466621554354No Hit
GTACTT861060.3463303128677241No Hit
TAATGA851630.3425374356578402No Hit
ACAGCC847730.3409688013928829No Hit
GGATTC836710.3365364040595933No Hit
GAAGAT822590.3308571435926197No Hit
GGCCGT817750.3289104282484163No Hit
GCCATA817380.3287616091002024No Hit
GACTTG815290.32792098201975095No Hit
CGTCAG813270.3271085099132859No Hit
GGATCG797750.3208661499665841No Hit
TTCACA793080.31898781098777623No Hit
ACCAGG779000.3133246390773663No Hit
CTGAGG766400.308256743759812No Hit
TACAGT765510.3078987733762705No Hit
AAGCGC755580.3039047892093407No Hit
AACATA743960.2992310635275962No Hit
AACTAG742490.29863980907388155No Hit
CATGAG728080.2928439065718214No Hit
AAGTTG727090.2924457147968707No Hit
GGTAAT726760.2923129842052205No Hit
GTCGAG722910.2907644606359678No Hit
TCCAGA718630.28904298508365567No Hit
CAAGGA709540.28538686060456286No Hit
AACTCT705540.2837780049481965No Hit
AGAACG701260.2820565293958843No Hit
GAGAAG683140.2747684132725443No Hit
CACCTA674920.2714622148987113No Hit
CGAGAG652380.26239631327508633No Hit
CGAAGC642980.25861550248262516No Hit
TAGAGC635190.25548225609185155No Hit
GAACCG591110.2377526667586932No Hit
TCTCTG583100.2345309333068194No Hit
TCGGAC579920.23325189306000804No Hit
CAACGG559530.22505075135168007No Hit
CGCTTG553350.22256506936259393No Hit
GGACTA550230.22131016195062808No Hit
AGTGCA542580.21823322550782723No Hit
TATGGT540720.21748510762761683No Hit
GACACG539470.21698234023500232No Hit
ACGAAC533290.21449665824591616No Hit
GAGTCG531730.21386920453993324No Hit
CTGTTC524540.21097728649761455No Hit
GCACAT517130.2079968813941957No Hit
GCTGTC515660.20740562694048104No Hit
TACGGC514840.20707581153092589No Hit
CCTCGG511020.20553935437909593No Hit
ACACGA509020.20473492655091272No Hit
AAGGCG505050.203138137311969No Hit
TACGAA494610.1989390240488526No Hit
CGTGAA493060.19831559248201058No Hit
GACTAA489840.1970204636786356No Hit
GGAGGC477280.19196865691764492No Hit
TCCACG471360.1895875505462226No Hit
TTCCAT465410.1871943777573775No Hit
TTGAAT465020.18703751433088175No Hit
CGAATT458430.18438692463701803No Hit
TATTCT443760.17848644651729406No Hit
AATTGC438960.1765558197296543No Hit
CGAGCC432890.17411438127111822No Hit
AAGAGG430550.17317320071214387No Hit
AGGTGT429540.17276696465891134No Hit
TTAGTA425220.17102940055003557No Hit
ACCTGC415410.16708368205279683No Hit
GTAAGA414400.1666774459995643No Hit
CAGCCT406800.16362062025246807No Hit
ATTCGA404390.1626512847195073No Hit
CATACG395220.15896298312728718No Hit
CCTGCT393190.1581464888816812No Hit
ACTAGC390420.15703235633964743No Hit
ATTGCT389910.15682722724346074No Hit
GTTGGC389470.1566502531212604No Hit
ATCCGG386500.15545567779640831No Hit
TACTCG379270.15254767119752596No Hit
GATTCA370470.1490081887535198No Hit
CTCCGC365540.14702527415704814No Hit
CTTCAC360560.1450222488648719No Hit
CCTGAC358110.14403682477534743No Hit
GAGATA353120.1420297773440303No Hit
CCATGA346100.13920623566710716No Hit
CTATGC343510.1381645016296099No Hit
TGACAC341980.13754911434104972No Hit
TGCATA340140.13680904073912117No Hit
TTAGCG330190.13280701229390962No Hit
AACCTT326420.13129066583778423No Hit
TGGCAT324420.13048623800960102No Hit
AGGACT313670.12616243843311617No Hit
TCGTGG313670.12616243843311617No Hit
CACATT306870.12342738381729321No Hit
AGAGGT303550.12209203362250905No Hit
TCTCGC297140.1195138424331818No Hit
AGTTCC296870.11940524467637709No Hit
CGTCTC291140.11710055894863214No Hit
CGACAA288400.11599849282402112No Hit
GCATAA280110.11266413947620166No Hit
CATTAA280000.11261989594565157No Hit
TGCGGA278240.11191199945685033No Hit
CATAAC277320.11154196265588605No Hit
AGTAGG264480.10637753599894972No Hit
TCCTCC262200.10546048827482085No Hit
GGCAGG260810.10490141093423351No Hit
ACCGCT259570.10440266568075991No Hit
GCCTGG258850.10411307166261397No Hit
AATCGG258230.10386369903587717No Hit
GAGCAC257000.10336897592154448No Hit
CCGCTC250510.1007586076190899No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)