FastQCFastQC Report
Sat 16 Sep 2023
EGAF00001835984

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001835984
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences400301916
Sequences flagged as poor quality0
Sequence length6
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTACGT53792291.3437929685053018No Hit
CAGTAG45891081.1464116999130227No Hit
CAGGAC43751241.0929560477047529No Hit
GAGAAG42052711.0505248243678154No Hit
TGTGAT39826940.9949225424142111No Hit
GGTACC39586660.9889200730180866No Hit
ATGCCG39175480.9786483260299959No Hit
ACGAAC37953940.9481328587995066No Hit
GGCTAC37283590.9313867485960272No Hit
AGGACT37006250.9244584779854015No Hit
GCACAT36265170.9059454514327131No Hit
CGTGAA34425190.8599806452088028No Hit
CCTGCT33789640.8441038788332954No Hit
CGTACT33096480.8267879487241826No Hit
GTGGTG32733680.8177247895061286No Hit
GAATAC32645990.8155341929465059No Hit
ACAGCC32464870.8110096080579339No Hit
CTGTTC32064870.8010171502651514No Hit
CGAATT31631910.7902013139502435No Hit
CCGTCG31356550.7833225060056921No Hit
TTGAAT31046420.7755751036675028No Hit
GTCTCA31011530.7747035115365273No Hit
CCTTAT30746880.7680922516493776No Hit
GGACTA30193300.7542631896870562No Hit
ACTAGC29625840.7400873894393251No Hit
TCGTGG29149500.72818787107679No Hit
GAGATA28931430.7227402328996096No Hit
GTAAGA28760310.7184654594558573No Hit
TGCATA28591120.7142388996209551No Hit
CAGATC28537400.7128969125393845No Hit
TAGCTT28086450.7016316654352461No Hit
TCTCTG27957650.6984140940259701No Hit
TACGGC27575750.688873794948311No Hit
CATACG27348450.6831955808075623No Hit
GAAGAT27200620.6795026182187947No Hit
AACTAG27191990.6792870309419153No Hit
CTGTAT27066840.6761606407099985No Hit
CAGCCT27048130.6756932434967411No Hit
AACCTT26792250.6693010682466981No Hit
TGATAA26762940.668568870901932No Hit
GGCCGT26724540.6676095949538249No Hit
TTAGTA26721150.6675249088740309No Hit
CACATT26540630.6630153126721482No Hit
ATCCGG26483300.6615831436589976No Hit
CCTCGG26373260.6588342185202032No Hit
GCTTCT26083940.6516066737986835No Hit
CGAGCC25266760.6311925821509183No Hit
ACTTGA25112800.6273464851464763No Hit
AAGAGG25015150.6249070763878133No Hit
CCATGA24994910.6244014580234984No Hit
TTCTAC24264620.6061579780197704No Hit
ACACGA23767380.5937363537375624No Hit
ACCAGG23221260.5800936511130764No Hit
GTACTT23086060.5767162003791159No Hit
CAGTCC22832930.5703927232763982No Hit
CGTTGC22557090.5635019243824954No Hit
GCGCAG22340990.5581034990599446No Hit
GAGTCG22170420.5538424652456573No Hit
GGCCAG22096730.5520016047087819No Hit
AAGCGC22094350.5519421495849148No Hit
GAGCAC22056490.5509963634548279No Hit
TCAATG21784610.5442044898930737No Hit
AAGGCG21097130.5270304526846182No Hit
ATTCAT21097030.52702795457017No Hit
CTTAGC20969450.523840860157162No Hit
CTGGAG20967830.5238003907031011No Hit
CCACTT20865490.5212438203768177No Hit
CATGAG20829340.520340752003795No Hit
ACTAAT20759120.5185865760382721No Hit
CAACGG20744730.5182270973691767No Hit
TGTGTG20607340.5147949379288007No Hit
AGTTAG20450550.5108781442854747No Hit
CCAGCG20286920.5067904796138922No Hit
TCTTCA20201600.5046590883666917No Hit
GATCAG19652820.49094993589788366No Hit
ATGGCC19461290.4861652972952545No Hit
AACATA19249110.480864798059073No Hit
TCATGT19131900.4779367581143429No Hit
AGACGG19085580.4767796315019387No Hit
GACTTG18865560.4712832800930186No Hit
GGTAAT18650600.46591333327517725No Hit
GTCGAG18509180.4623804998225389No Hit
GGTGGT18426590.4603173070997742No Hit
GGAGGC18376120.4590565087377698No Hit
GCCATA18234800.4555261733995797No Hit
GCTGTC17768910.44388770799688104No Hit
TACAGT17210240.42993149200914643No Hit
CATAAC17069830.4264238895124349No Hit
CTGAGG16842490.4207446761259069No Hit
GTCTGC16778000.4191336421182656No Hit
TCCACG16617050.4151129269138946No Hit
CCGGAA16249900.4059410997173443No Hit
CTATTA15954060.39855067793380233No Hit
CAAGGA15785530.3943406056542582No Hit
CGATGT15764260.393809256711127No Hit
TCCAGA15523220.3877878016451962No Hit
AACTCT15512660.3875240007594668No Hit
TAGAGC15453910.38605635852115183No Hit
GTACGC15073000.3765407907765298No Hit
GACACG14970630.3739834710159119No Hit
TTAGGC14917160.3726477292204617No Hit
GCTCAA14893820.3720646693082528No Hit
CGAAGC14768440.3689325334131051No Hit
GACCGA14745610.368362213884582No Hit
TATGGT14594150.36457856974134495No Hit
ACAGTG14538620.3631913667882619No Hit
TGCGAC14431990.3605276273521508No Hit
TTCTGT14282320.35678869945753644No Hit
TGACCA14230880.3555036693853846No Hit
ACCGCT13861360.3462726368764121No Hit
ATTGAA13747010.3434160430049003No Hit
TCGGAC13717240.3426723543336725No Hit
TAATGA13636110.34064563408185133No Hit
GAACCG13531290.33802711051725265No Hit
ACCTGC13462700.33631365381723527No Hit
GACTAA13413530.3350853309430575No Hit
GCATAA13298040.3322002585668363No Hit
ATGTGA13165720.32889475352898384No Hit
AGTGCA13137550.32819103468892713No Hit
TGGCAT13039640.3257451308326988No Hit
GTTGGC12882260.3218135983141285No Hit
CGCTTG12839100.3207354121182872No Hit
AATCGG12513400.312599053360514No Hit
CGTCAG12213960.305118699456837No Hit
TTCGTT12079460.30175873552401383No Hit
CTATGC11958930.29874775817960364No Hit
AGCCTA11950800.29854466147496533No Hit
AGGTGT11534690.28814975744457844No Hit
TATAAG11516480.287694850803562No Hit
GTTACA11124190.2778949976347353No Hit
CAAGCT11112460.2776019688099619No Hit
AGGTCA11019910.27528996388815685No Hit
GATCCT10946520.2734565976946261No Hit
CTAGTT10895370.272178812154374No Hit
TACGAA10808790.27001594466512624No Hit
TTCCAT10767080.2689739811287838No Hit
GGATTC10263040.2563824850640985No Hit
GCCAAT10235420.25569250585350684No Hit
ATCAGC9983030.24938751479770585No Hit
TATTCT9863770.2464082635067877No Hit
GGATCG9825390.24544948718157023No Hit
CTCCGC9823040.24539078149203764No Hit
TAAGTC9725470.24295337122493313No Hit
CTACCG9575940.2392179406905462No Hit
CGAGAG9523230.23790118456490225No Hit
CCGCTC9442650.2358882039425462No Hit
TATTGG9330880.23309606142379793No Hit
AACAAT9248440.23103661587270544No Hit
CACCTA8972330.22413907206979244No Hit
CCTGAC8911340.2226154720678379No Hit
TACTCG8645160.21596599102963074No Hit
TGCGGA8306880.2075153694742745No Hit
AGTAGG8265710.20648689575595236No Hit
GACCAT8080630.20186338553523187No Hit
TTAGCG7958600.19881493647409873No Hit
CTTCAC7911300.19763332834010217No Hit
GCGTAT7888130.19705451522245526No Hit
TGACAC7794630.1947187782133923No Hit
TCTCGC7661370.1913897908997268No Hit
AATTGC7613590.19019619181637892No Hit
ATTGCT7600420.18986719014355155No Hit
TCCTCC7433280.18569184165483735No Hit
CGTCTC7376840.18428190586027574No Hit
AGAGGT7371050.18413726503372518No Hit
ATACCT7248210.18106858124556166No Hit
GCGGCT7128590.17808033674263005No Hit
CATTAA7042620.17593270775151623No Hit
CCGACT6855430.17125648731593882No Hit
ATTCGA6772320.1691803043980434No Hit
AGTTCC6669630.1666149906711913No Hit
AAGCAT6609550.16511412351071533No Hit
CGACAA6176620.15429903663014194No Hit
TTCACA6168430.1540944410568347No Hit
GATTCA6155990.15378367561947917No Hit
ACGCTA6150040.15363503780981153No Hit
GGCAGG6138970.15335849654039627No Hit
GCCTGG5995920.1497849438222524No Hit
AGAACG5894460.1472503569031131No Hit
AGATGC5815320.14527334912881107No Hit
GAGCCA5635970.14079298086597217No Hit
AAGTTG5446570.1360615521010896No Hit
AGTCAA5297730.1323433585563952No Hit
GAGTGA4891450.12219401917626595No Hit
GGCGTT4298580.10738344804724842No Hit
AAAAAA4278020.1068698357166994No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)