FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001835986

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001835986
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22160217
Sequences flagged as poor quality0
Sequence length6
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAC4389041.9805943236025172No Hit
GCTTCT4080401.841317709118101No Hit
AGGACT3757361.6955429633202599No Hit
GAGCAC3749521.692005091827395No Hit
GCACAT3604461.6265454440270148No Hit
TCGTGG3586591.6184814435707013No Hit
GATCAG3456601.5598222706934683No Hit
TAGCTT3443291.55381601181974No Hit
TCAATG3393751.5314606350650808No Hit
ACTTGA3291261.485211087960014No Hit
ACTAGC3253201.4680361658913357No Hit
TTAGTA3140101.4169987595338078No Hit
CGTTGC3130241.4125493446205875No Hit
GTAAGA3003991.355577880848369No Hit
CCATGA2902531.3097931306358597No Hit
GGTAAT2770791.2503442543003978No Hit
AAGAGG2753311.24245624490049No Hit
CCTGCT2744601.2385257779741055No Hit
AAGCGC2646711.194352022816383No Hit
GAATAC2641231.1918791228443295No Hit
CCTTAT2621341.182903578967661No Hit
CAGTAG2547971.149794697407521No Hit
GGCCGT2481331.1197227897181692No Hit
CGTACT2423871.0937934407411263No Hit
CAGATC2375421.071929936426164No Hit
CTTAGC2335061.0537171183838137No Hit
TGATAA2253371.0168537609536947No Hit
GCCAAT2165640.9772647984448889No Hit
TCCAGA2144820.9678695835875615No Hit
TTCTAC2040630.9208528959802154No Hit
GAGATA2038720.9199909910629486No Hit
ATGGCC2017530.9104288103315955No Hit
TCTTCA2004960.9047564832059182No Hit
GCTCAA1970080.8890165651356212No Hit
CCACTT1943790.8771529628974302No Hit
CCAGCG1941840.8762730076154037No Hit
CACATT1897710.8563589426944691No Hit
GTGGTG1884990.8506189267009434No Hit
CGATGT1847290.8336064579151007No Hit
CGAATT1730460.780885855043748No Hit
TGTGTG1721480.776833548155237No Hit
CTGGAG1669190.7532372088233612No Hit
ATGCCG1664260.751012501366751No Hit
CTGAGG1662850.7503762260089782No Hit
GGCCAG1641610.7407914823216758No Hit
TGACCA1639750.7399521403603584No Hit
TGTGAT1607110.7252230427165943No Hit
GTCGAG1598320.7212564750606909No Hit
TTACGT1580440.7131879620131879No Hit
GACCGA1551790.7002593882541854No Hit
AACTCT1524350.6878768380291582No Hit
GGACTA1511760.682195485721101No Hit
TTGAAT1506060.6796233087428702No Hit
CAAGGA1505010.679149486667933No Hit
ATTCAT1443250.651279723479242No Hit
TACTCG1421940.6416633916536106No Hit
TCATGT1420260.640905276333711No Hit
ACAGTG1406430.6346643627181088No Hit
GTACTT1365880.6163658054431507No Hit
TTAGGC1357580.6126203547555513No Hit
AAGTTG1348360.6084597456784832No Hit
GCGCAG1343960.6064742055549366No Hit
ATTGAA1320490.5958831540322913No Hit
TACAGT1257930.5676523835484102No Hit
AACTAG1211740.5468087248423605No Hit
TAGAGC1210960.54645674272955No Hit
CCGGAA1198850.5409919947986069No Hit
TATAAG1135880.5125762080759407No Hit
CATTAA1129570.5097287630351274No Hit
ACTAAT1119850.5053425243985652No Hit
TTCGTT1071670.48360086004572966No Hit
GGTGGT1065460.48079854091681506No Hit
GAGAAG1047570.47272551527812207No Hit
CATAAC1042920.47062716037482843No Hit
CACCTA1040200.4693997355711814No Hit
AAGGCG1035830.46742773322120446No Hit
CCGACT1026550.463240048596997No Hit
GGTACC971000.43817260453722096No Hit
CGTGAA969780.4376220684120557No Hit
GGCGTT964140.4350769669809641No Hit
CAGCCT952580.4298604115654644No Hit
AGACGG950470.4289082548243999No Hit
GTCTGC925970.4178524064091972No Hit
CAACGG917770.41415208163349665No Hit
CAGGAC916910.4137639987911671No Hit
TACGGC911210.4111918218129362No Hit
TGCATA905870.40878209811754096No Hit
ACAGCC894050.4034482153311044No Hit
AACCTT892250.4026359489169263No Hit
CATACG880560.397360729815958No Hit
AGGTGT864670.3901902224152408No Hit
GTCTCA857060.3867561405197431No Hit
AATCGG856340.38643123395407186No Hit
ACCTGC851630.38430580350363897No Hit
AATGTA846950.38219391082677573No Hit
AGATGC839010.3786109134220121No Hit
CTCCGC835000.3768013643548707No Hit
CAAGCT828950.3740712466849941No Hit
CCTCGG819900.3699873516581539No Hit
AACAAT815910.3681868277733923No Hit
CTGTAT811410.3661561617379469No Hit
GAAGAT802950.3623385095913095No Hit
AGCCTA799600.36082679154270014No Hit
AGGCTT792940.3578214058102409No Hit
ATCCGG756120.3412060450491076No Hit
CTAGTT732070.33035326323744935No Hit
TATTCT725430.3273569026873699No Hit
CTGTTC705010.3181421914776376No Hit
AGTTAG697460.31473518512927917No Hit
ACGAAC690400.31154929574922485No Hit
CGAGCC677950.3059311197178259No Hit
GGATTC676470.3052632562217238No Hit
CAGTCC654350.29528140450971213No Hit
GAGTCG651830.29414423152986274No Hit
AGAGGT647080.29200075071467035No Hit
GATTCA632850.28557933345147296No Hit
TCTCTG612580.2764323111095889No Hit
ACACGA611460.2759269008963225No Hit
CCGTCG608380.2745370228098398No Hit
CGTCTC605050.2730343299436102No Hit
ACCGCT600630.2710397646376838No Hit
GCATAA599230.2704080018711008No Hit
AGTTCC591510.26692428147251446No Hit
TTCTGT571370.25783592281609874No Hit
TCCACG531810.23998411206893866No Hit
ATGTGA506610.2286123822704444No Hit
GACTTG503790.22733983155489856No Hit
AGTCAA490110.22116660680714453No Hit
ATCCAC488330.2203633655753461No Hit
AATTGC487260.2198805183180291No Hit
TTAGCG475960.2147812902734662No Hit
TCTCGC474620.21417660305402242No Hit
ATTCGA473940.2138697468531107No Hit
TGCGAC472620.2132740848160467No Hit
GCCATA453310.20456027122839093No Hit
TTCACA447640.20200163202372975No Hit
CGCTTG446590.20152780994879246No Hit
GCCTGG444040.20037709919537341No Hit
TGGCAT426390.1924123757452375No Hit
TAATGA421620.19025986974766537No Hit
AGGTCA415810.18763805426634586No Hit
AAGCAT414720.18714618182664908No Hit
CGAAGC411690.18577886669611585No Hit
CCTGAC409130.18462364335150688No Hit
GTACGC402500.1816317953926173No Hit
CTTCAC400270.18062548755727437No Hit
TATGGT394380.17796757134643582No Hit
GTTACA389090.17558041060699No Hit
AGAACG389040.1755578476510406No Hit
ATCAGC385950.1741634569733681No Hit
TAAGTC380220.1715777422215676No Hit
AGTAGG376490.16989454570774284No Hit
ATTGCT371640.16770593898065167No Hit
CTCGTC370380.16713735249072698No Hit
CGTCAG366230.1652646271469273No Hit
ATACCT365260.16482690580150908No Hit
TCAAGC355430.16039102866185834No Hit
ACGCTA352890.15924483049962912No Hit
CTACCG349140.15755260880342462No Hit
TGACAC339350.15313478202853337No Hit
CGAGAG337400.15225482674650703No Hit
GACCAT317170.14312585476938244No Hit
GGAGGC307690.13884791832137744No Hit
GGCAGG286440.12925866204288522No Hit
CTATGC272140.1228056566413587No Hit
ACGTAA267590.12075242764996388No Hit
GATCCT262450.11843295577836627No Hit
GAGTGA248910.11232290730727051No Hit
GAACCG248040.11193031187375106No Hit
TCGGAC247970.11189872373542191No Hit
TATTGG247230.11156479198737089No Hit
CGACAA240680.10860904475800033No Hit
AGTGCA239130.10790959312356914No Hit
TACGAA231660.10453868750472976No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)