FastQCFastQC Report
Tue 5 Sep 2023
EGAF00001835988

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001835988
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences397556019
Sequences flagged as poor quality0
Sequence length6
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGATAA44739971.1253752392565335No Hit
GGCTAC43783411.1013142276183223No Hit
GGTACC42769011.0757983266755673No Hit
TTACGT40569831.0204808394562377No Hit
CAGGAC38887940.9781751034185702No Hit
TAGCTT38292400.9631950761635935No Hit
CCGTCG37921330.9538612972175878No Hit
CGTACT37219040.9361961137859165No Hit
TTGAAT36249660.9118126318696234No Hit
AGTTAG35651150.8967578981617682No Hit
GGCCGT35424750.89106310323527No Hit
CAGTCC35021650.8809236516678168No Hit
GTACTT33901770.8527545397319213No Hit
AAGCGC33327600.8383120467860404No Hit
GTGGTG33277690.8370566262260515No Hit
TCGTGG33207370.8352878188972911No Hit
GTAAGA32954430.8289254450955753No Hit
GAAGAT32913560.8278974138736408No Hit
AACTAG32907320.8277404548615324No Hit
CCACTT31535200.7932265767054075No Hit
AACTCT31256600.7862187592737716No Hit
AACCTT31146230.7834425467471038No Hit
GGTAAT31002200.7798196610878126No Hit
CAAGGA30546110.7683473156018297No Hit
TACGGC30378290.7641260237088751No Hit
CCTTAT29691310.7468459432380019No Hit
GATCAG29685110.746689990373407No Hit
CATACG29673050.746386636898082No Hit
TGCATA29466710.7411964249496119No Hit
GCACAT29135830.7328735727178112No Hit
CCTCGG29045360.7305979185791173No Hit
GCTTCT28315360.712235726457458No Hit
CCAGCG28234270.7101960139106837No Hit
CAGTAG28084370.706425476103784No Hit
CGAGCC28005570.7044433655021584No Hit
ATTCAT27999360.704287161100685No Hit
CAACGG27540360.6927416183830938No Hit
ACTTGA27485730.69136747241651No Hit
TTCTAC27438900.6901895252150615No Hit
GACTTG27043650.6802475300971359No Hit
GCTCAA26924330.6772461920643189No Hit
TAGAGC26860990.675652957476667No Hit
CTGAGG26710120.6718580205925645No Hit
GAATAC26481950.6661187036385934No Hit
TGTGAT26426400.6647214162791987No Hit
CTGTTC26423400.6646459552156849No Hit
TACAGT25895710.6513726056805091No Hit
AAGGCG25600820.643955034673994No Hit
TCTTCA25241390.6349140446544214No Hit
CAGCCT24996840.6287627102936655No Hit
ATCCGG24879630.6258144465421865No Hit
CCTGCT24446910.614929942740975No Hit
CAGATC24438630.6147216702056774No Hit
AGGACT24146710.6073788056520407No Hit
ATTGAA24026240.6043485408782101No Hit
GAGAAG23922590.6017413611338129No Hit
GCCATA23871310.600451480021486No Hit
ACAGCC23823490.5992486306690781No Hit
ACTAAT23637370.5945670262886902No Hit
ACACGA23300110.5860836935284836No Hit
CTTAGC23194640.5834307340722213No Hit
TTAGTA23167230.5827412714885849No Hit
CGTGAA23086690.5807153934701212No Hit
GAGATA22894860.575890161532179No Hit
CGTTGC22640660.5694960940837874No Hit
GCCAAT22415780.5638395327628029No Hit
GAGCAC22356890.5623582320860296No Hit
CGAAGC22161160.5574349007655195No Hit
ACGAAC22031260.5541674367153777No Hit
ATGGCC21902900.5509387093445063No Hit
CACATT21820200.5488584993603128No Hit
GCGCAG21576030.5427167234009354No Hit
CTGGAG21497080.540730839746134No Hit
GTCTGC21271610.5350594377493251No Hit
TATAAG21192140.533060474176848No Hit
TCATGT21166370.5324122636412656No Hit
CTGTAT20856080.524607325842047No Hit
CGAATT20842450.5242644810768164No Hit
CCATGA20757730.5221334606431904No Hit
CGATGT20704680.5207990575033905No Hit
AAGAGG20614660.5185347225242237No Hit
TCCACG20450440.5144039839074855No Hit
TCCAGA20050670.5043482941205325No Hit
GAGTCG19920100.5010639770995392No Hit
TCAATG19574240.4923643226239269No Hit
ACTAGC18943170.47649058484007No Hit
GGCCAG18532910.46617103286769757No Hit
ATGCCG18383690.46241759956852774No Hit
GTCTCA18200540.4578107016410183No Hit
CTAGTT18030760.4535401085199014No Hit
TGGCAT17562890.44177145259118816No Hit
GGACTA17537690.44113757965767336No Hit
GTCGAG17498530.44015256124194163No Hit
GCATAA17425070.4383047713333702No Hit
CCGGAA17143760.43122878740769366No Hit
TGACCA15918200.40040143374109No Hit
TCTCTG15594570.3922609457461138No Hit
TACTCG15177160.38176154490570047No Hit
GGTGGT15017350.37774173405232736No Hit
TTAGGC14579580.36673020412753454No Hit
AAGCAT14441520.36325748598463553No Hit
TCGGAC14141210.355703581990039No Hit
ACAGTG14009580.3523926020599376No Hit
GGCGTT13934640.3505075846933662No Hit
CGAGAG13797890.34706781788153485No Hit
AGTGCA13565850.341231156155631No Hit
TGTGTG13413600.3374015071823124No Hit
GAACCG13166390.33118326401190773No Hit
AGTTCC13146660.33068698175086614No Hit
TTCTGT13043040.32808055661710406No Hit
TATGGT12736960.3203815158436829No Hit
CGACAA12645120.31807140115265115No Hit
TAATGA12578580.316397674763918No Hit
TAAGTC12326580.3100589454287699No Hit
TACGAA12117740.3048058492607051No Hit
CCGACT11867760.29851793037498947No Hit
AGACGG11582330.29133831325542076No Hit
GTTACA11372360.28605679342009915No Hit
TTCCAT11221590.28226437190478054No Hit
AGGTGT11024600.27730934693759474No Hit
TCAAGC10996080.2765919637604581No Hit
AGTAGG10978550.2761510196126599No Hit
CTCCGC10955860.2755802824356182No Hit
GATCCT10705670.2692870812754567No Hit
TTAGCG10633930.2674825557099665No Hit
GTACGC10607810.26682554138364084No Hit
CTCGTC10211180.2568488341764988No Hit
GACCGA10208740.25678745917817436No Hit
AATCGG10133170.25488659498826505No Hit
TATTGG9946810.250198953722796No Hit
CCTGAC9561590.24050925009388427No Hit
CTATGC9345250.23506750126703527No Hit
AGCCTA9169260.23064070374444515No Hit
ACCGCT9050350.2276496787236417No Hit
AACAAT9033960.22723740977997872No Hit
ACGCTA8813290.22168674548479167No Hit
CGTCAG8667890.21802939927316253No Hit
GGATCG8659630.2178216298116216No Hit
GAGCCA8632660.21714323485063372No Hit
GATTCA8467140.21297979643970627No Hit
CTTCAC8440610.21231246910136709No Hit
TTCGTT8403500.211379015745703No Hit
GGAGGC8401640.21133222988632452No Hit
AAGTTG8377230.21071822836620166No Hit
TATTCT8305600.20891646970637362No Hit
CGCTTG8294100.2086272022962379No Hit
GCCTGG8177070.2056834662085697No Hit
CACCTA8157850.20520001232832547No Hit
CATAAC8144700.20486924133325726No Hit
ACGTAA8058210.2026936988721582No Hit
GGATTC8010520.20149411949916926No Hit
TGCGAC7849180.1974358235034042No Hit
AGGCTT7809480.19643722209623996No Hit
ATACCT7691060.19345852238247713No Hit
AGAGGT7683280.19326282669109834No Hit
CGTCTC7648520.1923884845018533No Hit
TGACAC7508810.1888742627740218No Hit
GACCAT7386540.18579872136208306No Hit
ATGTGA7358250.18508712353314918No Hit
ACCTGC7344340.18473723573532413No Hit
GAGTGA7309580.1838628935460791No Hit
AGATGC7143780.17969241210255704No Hit
AATTGC7115390.17897829890483938No Hit
ATCAGC7109500.1788301436834742No Hit
CAAGCT6808190.17125108600103978No Hit
CATTAA6682550.16809077666108735No Hit
GGCAGG6463350.16257708828702203No Hit
ATTCGA6461580.16253256625954896No Hit
ATTGCT6324730.15909028407893377No Hit
AGGTCA6294830.1583381888125809No Hit
TCTCGC6061480.15246857575560943No Hit
TTCACA5945240.14954471108133316No Hit
ATCCAC5698960.1433498608406178No Hit
AGTCAA5570070.14010780201519224No Hit
AGAACG5545410.1394875120731099No Hit
AATGTA5442520.13689944913146943No Hit
CTACCG5426880.13650604545368483No Hit
AAAAAA5290750.13308187392831297No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)