FastQCFastQC Report
Mon 4 Sep 2023
EGAF00001835992

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001835992
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences388271773
Sequences flagged as poor quality0
Sequence length6
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCCAG59643751.5361340727696937No Hit
GAATAC45727901.17772918815811No Hit
GGCTAC43543551.1214709136221448No Hit
TCAATG42751721.1010772086179956No Hit
GTACTT42335101.0903470956154209No Hit
GCTTCT38593500.9939816047354028No Hit
CGAATT35642980.917990502492696No Hit
CCTTAT35355760.9105931066485227No Hit
CGTACT35226390.9072611621447948No Hit
AGGACT35143690.905131210761489No Hit
TCATGT33937910.8740761590207073No Hit
TGTGAT33888980.8728159592482145No Hit
GACACG33439950.8612511216466926No Hit
CAGTAG32800650.84478585055422No Hit
TCGTGG32462250.8360703058370407No Hit
ACCAGG32416970.834904112383158No Hit
GTGGTG32219060.8298069095020203No Hit
CATGAG32019450.8246659228560506No Hit
CGTGAA31896230.8214923725603921No Hit
CCACTT31864490.8206749039158199No Hit
ATGCCG31369360.8079227536326725No Hit
CGTTGC31109500.8012300188507394No Hit
ATGGCC30385920.7825941032288227No Hit
CTGAGG30177900.7772365157227126No Hit
CCTGCT29463800.7588447589776247No Hit
GAGATA29456220.7586495348967848No Hit
TGATAA29307250.7548127893396979No Hit
AACCTT29300430.7546371391772536No Hit
ACACGA29147360.7506947974814538No Hit
CACATT29051670.7482302866245185No Hit
CTTAGC28585680.7362286415809063No Hit
TTGAAT28261230.7278723812869086No Hit
TTACGT27600900.7108654792683062No Hit
TTCTAC27453040.7070573219341392No Hit
GAGCAC27354600.7045219843987989No Hit
GATCAG27338780.704114537834302No Hit
GCACAT27266260.7022467739368733No Hit
TAGAGC27019590.6958937496597261No Hit
GTAAGA26967120.6945423766357592No Hit
TAGCTT26922540.6933942117909251No Hit
CCGTCG26739460.6886789578700587No Hit
ATTCAT26629280.6858412548058187No Hit
GGCCGT26408430.6801532286510047No Hit
AACTAG26386770.6795953719767314No Hit
AGTTAG26239690.6758073036640755No Hit
GTCGAG26088930.6719244563781359No Hit
CTGTTC25981070.6691465052753139No Hit
GTTGGC25938460.6680490780873736No Hit
ACTAAT25860990.666053826168816No Hit
TACAGT25754680.6633157955574587No Hit
GGTAAT25341420.652672219878317No Hit
CAGTCC24909080.6415372358268238No Hit
CAGCCT24592150.6333746543043189No Hit
CAAGGA24586650.633233000947509No Hit
TATGGT24568990.6327781648963702No Hit
CAGATC24516600.631428852284866No Hit
CAGGAC24452280.629772280664863No Hit
AACATA24323670.6264599100795308No Hit
GAAGAT23840680.6140204273875969No Hit
ACTAGC23765770.6120911086678454No Hit
ACTTGA23149850.5962279931175939No Hit
TCTTCA22603250.5821502249662636No Hit
CCTCGG22567120.5812196911878011No Hit
CTGTAT22426100.577587699119194No Hit
AATCGG22379610.5763903419268133No Hit
ACGAAC22342320.5754299321676417No Hit
ACAGCC22246570.5729638760013595No Hit
TACGGC22231380.5725726551850062No Hit
GTCTCA22103300.5692739348322393No Hit
GGACTA22052990.567978192944765No Hit
CATACG21933440.5648991640708324No Hit
CTGGAG21773110.5607698399440435No Hit
GAGAAG21406890.5513377867929636No Hit
TGGCAT21152470.5447851600584933No Hit
TGCATA20922810.5388702309812257No Hit
TTAGTA20877170.5376947656712608No Hit
CCAGCG20633670.5314233852379477No Hit
CAACGG20501730.528025249983856No Hit
CGAGCC20407080.5255875244889358No Hit
GCTGTC20338030.5238091309820763No Hit
AACTCT20304880.5229553475678491No Hit
GGTACC19983110.5146681110913515No Hit
GGCGTT19977060.5145122923988605No Hit
AAGCGC19556540.5036817342887298No Hit
ATTGAA19479060.5016862248186144No Hit
GTCTGC19351160.49839214039388846No Hit
GAGTCG18573010.47835076592085923No Hit
CCATGA17666910.455014019265315No Hit
TCCAGA17591330.4530674445911885No Hit
GCTCAA17122970.441004759828369No Hit
AGACGG17001100.4378659789930184No Hit
AAGGCG16616490.4279602885270776No Hit
TATAAG16518760.4254432371523438No Hit
TCCACG16443740.4235110853654561No Hit
CCGGAA16186050.4168742392715733No Hit
GGTGGT16138290.4156441730313473No Hit
AAGAGG16088610.4143646568920167No Hit
CCGACT15152490.3902547404598479No Hit
GCCATA14617080.3764651725017363No Hit
GACTTG14285570.36792708080790615No Hit
ATCCGG14016620.3610002316598997No Hit
ACCGCT13744700.3539968896992159No Hit
TCAAGC13558190.3491932955940117No Hit
AGTAGG13476290.3470839483353326No Hit
AAGCAT13008340.33503182318638447No Hit
GCGCAG12767390.32882611840031956No Hit
GCCAAT12297980.3167363907239273No Hit
CGATGT11968090.3082400223824666No Hit
AACAAT11966500.3081990716847706No Hit
AGCCTA11845480.3050821827318361No Hit
TACTCG11596630.2986730122150806No Hit
CGAAGC11548580.2974354769796773No Hit
TATTCT11420490.29413649907535255No Hit
GAACCG10651720.2743367079635738No Hit
TAATGA10557010.2718974371593064No Hit
TAAGTC10457890.26934458611803336No Hit
TGACCA10430420.26863709198865715No Hit
CTATGC10095890.26002121972436043No Hit
TCGGAC9361760.24111358720892645No Hit
GACCGA9117440.23482108754787076No Hit
TGTGTG8893460.22905244775545403No Hit
CTATTA8678680.22352075539624666No Hit
AGTCAA8625200.22214336966493828No Hit
CCTGAC8538860.21991966951457995No Hit
AATTGC8524100.21953952341521363No Hit
AATGTA8431050.2171430061695471No Hit
CTTCAC8393370.2161725518996201No Hit
TTAGGC8348300.215011767028452No Hit
GGATTC8165940.2103150568197498No Hit
CGTCAG8109720.20886710196159433No Hit
ATGTGA8107350.20880606224238712No Hit
TTAGCG8103310.20870201141302125No Hit
AAGTTG8043970.20717370046882083No Hit
TTCGTT7560920.1947326724675399No Hit
TTCTGT7548480.19441227832959157No Hit
TTCCAT7479100.19262538562132356No Hit
CAAGCT7350110.1893032280767935No Hit
GGCAGG7137030.1838153194824183No Hit
CGCTTG7104880.1829872912239747No Hit
ACAGTG7053630.18166733949006383No Hit
TGCGAC6879360.17717898849165117No Hit
CATTAA6865750.17682846082143602No Hit
GTACGC6810740.1754116697017787No Hit
AGGCTT6810730.17541141215022088No Hit
ATCCAC6790300.17488523431756137No Hit
ATTGCT6742750.17366057666004991No Hit
ATACCT6721940.17312461186819264No Hit
ACCTGC6717950.17302184879661597No Hit
TACGAA6670360.17179616093287317No Hit
AGTGCA6657010.17145232960316176No Hit
TCCTCC6624130.1706055000809961No Hit
CTAGTT6513040.167744359824993No Hit
CCGCTC6220990.1602225665783848No Hit
AGGTCA6206810.15985735846937293No Hit
AGAGGT6167160.15883616654255214No Hit
CACCTA6054920.15594540785739788No Hit
AGAACG5978560.1539787441617601No Hit
GCGTAT5853450.15075651662167056No Hit
GGATCG5836380.15031687611244407No Hit
CTCCGC5791700.1491661357520316No Hit
TATTGG5690410.14655739602270804No Hit
AGATGC5653390.14560394015559816No Hit
CTACCG5637610.14519752379733253No Hit
CTCGTC5615510.1446283348545144No Hit
GACCAT5591470.144009180909476No Hit
GAGCCA5528200.14237965220304594No Hit
CGTCTC5510770.14193073983773732No Hit
AGGTGT5510060.14191245367713093No Hit
ATCAGC5509980.14191039326466826No Hit
GACTAA5476060.14103677838048764No Hit
CATAAC5417420.13952649604533574No Hit
GTTACA5345410.13767186727735678No Hit
AGTTCC5338510.13749415670244977No Hit
TGACAC5337850.13747715829963256No Hit
ATTCGA5333910.13737568298584507No Hit
ACGCTA5045100.12993733644397581No Hit
TTCACA4974950.12813061226575437No Hit
GATCCT4848840.12488262956988119No Hit
GATTCA4822700.12420938979769719No Hit
GGAGGC4582870.11803253078610998No Hit
CGACAA4556850.11736238163262No Hit
TCTCGC4525440.11655341218945628No Hit
CGAGAG4519430.11639862370319669No Hit
ACGTAA4484750.1155054349006205No Hit
TCTCTG4210360.10843847770515114No Hit
GCGGCT4096520.10550651077074304No Hit
GCCTGG3968210.10220186673214589No Hit
GCATAA3890810.10020841767449318No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)