FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001835994

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001835994
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29497951
Sequences flagged as poor quality0
Sequence length6
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGAAT11153923.7812524673323917No Hit
AACATA9300603.1529647601624937No Hit
CGTGAA8257132.7992215459304277No Hit
TTACGT7728302.6199446870055483No Hit
GAGAAG7339042.4879829788855505No Hit
ACCAGG6790372.301980229067436No Hit
CAGCCT6381152.1632519492625097No Hit
AAGGCG5164061.750650409582686No Hit
TCAATG4854921.6458499100496844No Hit
GTAAGA4582941.5536468956776015No Hit
GACACG4399961.4916154684777936No Hit
GTTGGC4110241.393398477067102No Hit
TCTTCA4039031.3692578172633074No Hit
GCTGTC3813161.2926863970992426No Hit
GAGCAC3731211.2649048064389286No Hit
AGGACT3680371.2476697110250132No Hit
TTAGTA3676991.2465238687256617No Hit
CGAATT3561231.2072804650058575No Hit
CGTTGC3448711.169135442661763No Hit
CATGAG3393271.150340916899618No Hit
GAGATA3313201.1231966586424935No Hit
TCCAGA3232601.0958727268887252No Hit
GGCCGT3209841.0881569367309614No Hit
CTTAGC3163621.0724880517972248No Hit
GTCGAG3100711.051161146752193No Hit
GGACTA3066701.0396315323732146No Hit
ACTAGC2977141.0092701015063723No Hit
GGTAAT2922430.9907230505603594No Hit
CCTGCT2798070.9485641901025601No Hit
CTGGAG2784280.9438892891238445No Hit
AAGCGC2763310.9367803207755007No Hit
CACATT2729540.9253320679799082No Hit
GCACAT2621540.8887193554562485No Hit
GACTAA2562370.8686603350856472No Hit
TCGTGG2451170.8309628014501753No Hit
CCAGCG2439200.826904892478803No Hit
AACCTT2300950.7800372303825442No Hit
TACGGC2247280.76184274629787No Hit
TCTCTG2240800.7596459835464504No Hit
CCATGA2191170.7428210861154391No Hit
ACGAAC2178410.7384953619320881No Hit
GAGTCG2140400.7256097211633445No Hit
GTCTCA2070230.701821628220889No Hit
TGATAA2059330.6981264563087789No Hit
CCTCGG2055750.696912812689939No Hit
GTACGC1997570.6771894088508046No Hit
CGTCAG1947190.6601102564717122No Hit
CGTACT1929760.6542013714783105No Hit
ATCCGG1915460.6493535771349No Hit
ATTGAA1911450.6479941606791604No Hit
ACAGCC1868230.6333422955377477No Hit
ATTCAT1817020.615981767682779No Hit
CCGGAA1805080.61193402890933No Hit
CAACGG1804760.6118255467981488No Hit
ACTAAT1727020.5854711739130627No Hit
AAGAGG1716920.5820472072789056No Hit
GTCTGC1705630.5782198227937934No Hit
TACAGT1673580.5673546613457999No Hit
GGAGGC1652200.5601067002925051No Hit
GCGCAG1647420.5584862487567357No Hit
CTGTAT1617210.5482448594480341No Hit
GCTCAA1594330.5404883884985774No Hit
AACTCT1543720.5233312646020736No Hit
GAATAC1511370.5123644011748477No Hit
TAGAGC1507940.511201608545624No Hit
ACGCTA1490730.5053673050036593No Hit
CAAGGA1484410.5032247833078305No Hit
GCGTAT1463620.4961768361470259No Hit
TGCATA1453210.4926477774676621No Hit
TGCGGA1440940.4884881665170574No Hit
GGTACC1438930.4878067632562004No Hit
TCCTCC1427800.4840336198266788No Hit
GAAGAT1384280.4692800527060337No Hit
CATACG1384180.4692461520462896No Hit
CTGTTC1379570.46768333163208525No Hit
CCGCTC1373580.4656526821134118No Hit
ATGCCG1270900.4308434846881398No Hit
CTCGTC1248430.42322600644363395No Hit
GTACTT1242340.4211614562652165No Hit
CAGGAC1201630.4073604976833815No Hit
TACTCG1181920.4006786776478135No Hit
CCTTAT1147690.3890744818173981No Hit
ACACGA1107930.37559557950313227No Hit
ACCGCT1106200.37500909808955885No Hit
CAGTAG1073580.3639507028810238No Hit
AATCGG1058190.35873339134640236No Hit
AAGTTG1054810.35758754904705076No Hit
TGTGTG1028750.34875303711773065No Hit
GCGGCT1010820.3426746488256083No Hit
TTCTAC995410.33745055715903793No Hit
CGAGCC995070.3373352949159079No Hit
GACCGA989130.33532159572710657No Hit
GGCCAG972510.3296873060776323No Hit
CATTAA943710.31992391607132303No Hit
CTCCGC938310.31809328044514007No Hit
TCCACG900970.3054347740966822No Hit
GGATTC878380.29777661506048336No Hit
TGTGAT870110.29497303049964385No Hit
TATTCT861890.29218639626867643No Hit
ATGGCC843050.28579951197288245No Hit
AGAGGT836200.28347731678040955No Hit
AGTCAA791960.2684796649096068No Hit
TCTCGC778380.263875955316354No Hit
CGTCTC758980.2572992273259929No Hit
TTCGTT738060.25020720930752105No Hit
AGGTGT730140.24752227705578603No Hit
ATCCAC705110.23903694192183045No Hit
TGCGAC700590.2375046321013958No Hit
CACCTA692630.23480613958576307No Hit
AATGTA648810.2199508704858856No Hit
GATCAG645870.21895419108940822No Hit
CATAAC643020.21798802228670053No Hit
TATGGT603410.2045599709620509No Hit
TTCTGT602360.20420401403473754No Hit
ACTTGA598790.2029937604818721No Hit
ACCTGC585700.1985561641213656No Hit
TCATGT577390.19573901929662843No Hit
GTGGTG555970.18847749797943592No Hit
TAGCTT554050.18782660531234865No Hit
AGATGC545920.18507048167515092No Hit
AGGCTT542990.18407719234464795No Hit
CAAGCT540870.18335849835807239No Hit
TTCACA539930.1830398321564776No Hit
GGCTAC522010.17696483393032958No Hit
GCATAA521570.17681567102745543No Hit
AGAACG521310.1767275293121207No Hit
TAATGA518710.17584611215877333No Hit
TATAAG516460.17508334731453043No Hit
ATGTGA512280.1736662997372258No Hit
TAAGTC506980.17186956477078696No Hit
GTTACA491000.1664522393436751No Hit
AAGCAT487550.16528266658250262No Hit
CCACTT485380.16454702226605503No Hit
GATCCT478410.1621841462818892No Hit
ATACCT471760.1599297524089046No Hit
TCAAGC458820.15554300703801427No Hit
ATCAGC445710.15109863054555892No Hit
GCCATA442890.15014263194077446No Hit
CTAGTT440030.14917307307209235No Hit
CAGATC434290.14722717520277934No Hit
TGGCAT433070.14681358715390094No Hit
CTATTA426740.14466767539209757No Hit
AGTAGG425010.14408119397852415No Hit
AGGTCA420630.14259634508173127No Hit
ACGTAA413960.14033517107679785No Hit
CTACCG387380.13132437571680824No Hit
GAGTGA379910.1287919964339218No Hit
CCGTCG365390.12386962063907422No Hit
CTGAGG363710.12330008955537286No Hit
CTATGC359900.12200847441912151No Hit
GACCAT351570.11918454946243554No Hit
AGTTAG329770.11179420563821535No Hit
AGTTCC326220.11059073221729875No Hit
TGACAC301030.10205115602775258No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)