FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001835998

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001835998
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20666944
Sequences flagged as poor quality0
Sequence length6
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAGAT3854171.8648959420415518No Hit
NAAGAT3347121.6195524601992437No Hit
CAGGAC3283821.588923838957516No Hit
ACACGA3064121.4826188138894651No Hit
CGAGCC2991211.4473402550469001No Hit
NAGGAC2833981.371262243706665No Hit
TCCACG2782391.3462996754624197No Hit
AACCTT2677611.29560035581458No Hit
NCACGA2647421.2809924873266216No Hit
NGAGCC2603831.259900834879119No Hit
ACGAAC2457181.1889421096800765No Hit
NCCACG2407221.1647682405294175No Hit
TGCATA2381981.1525555011906936No Hit
NACCTT2334511.1295864545817709No Hit
CATACG2284961.1056109698656946No Hit
NCGAAC2140851.035881260432118No Hit
ATCCGG2128351.0298329544997074No Hit
NGCATA2057830.9957108317514192No Hit
CTGTTC1984570.9602629203427464No Hit
NATACG1939160.9382906345514848No Hit
NTCCGG1836130.8884380777341827No Hit
GGTACC1760710.8519450190603893No Hit
NTGTTC1724260.8343081589614797No Hit
NGTACC1526130.738440090610397No Hit
TTGAAT1389270.6722183986176186No Hit
CTATTA1344610.6506090111823015No Hit
TGATAA1319390.6384059491330697No Hit
AACTAG1257740.60857570427442No Hit
ATTCAT1253520.6065337961916382No Hit
NTGAAT1203490.5823260565277576No Hit
GAGTCG1189710.575658404067868No Hit
GCACAT1185540.5736406892088158No Hit
NTATTA1181100.5714923309416235No Hit
GAGAAG1171280.5667407818011216No Hit
NGATAA1148930.5559264107939713No Hit
TCAATG1112940.5385121283533744No Hit
NTTCAT1111580.5378540726679281No Hit
GGATCG1090530.5276687254777485No Hit
NACTAG1087200.5260574567773543No Hit
ACTAAT1073310.5193365792252594No Hit
NCACAT1039350.5029045416680861No Hit
TTAGTA1033530.5000884504259556No Hit
NAGTCG1022920.49495464835052533No Hit
TTACGT1020260.49366756884810836No Hit
ACAGCC1019560.49332886371589335No Hit
NAGAAG1012390.4898595554330626No Hit
TATTGG1006750.4871305597963589No Hit
CGAGAG998550.4831628711046974No Hit
AAGCAT984160.4762000613153062No Hit
TACGGC968710.46872435518284655No Hit
TCAAGC967590.4681824269713026No Hit
AAGAGG961510.46524053096577805No Hit
NCAATG958310.46369216464708085No Hit
AGGACT947930.458669651400807No Hit
NGATCG946780.45811320725502525No Hit
CAACGG939960.45481325153830193No Hit
NCTAAT936090.4529406960216276No Hit
GTCTGC927800.44892945952725277No Hit
CGTTGC923250.44672787616785525No Hit
TCGTGG914870.4426730918707672No Hit
CTGTAT902040.4364651106617408No Hit
CCATGA901430.4361699533322392No Hit
NCAGCC889890.4305861572954376No Hit
NTAGTA887940.42964262156998156No Hit
NTACGT885940.42867489262079583No Hit
GATCCT872710.4222733656219323No Hit
NATTGG868640.4203040372103394No Hit
NGAGAG866330.41918631027402986No Hit
NAGCAT862990.41757020292888974No Hit
CCTCGG846280.4094848275584431No Hit
GAGCAC844560.4086525806621433No Hit
CCTGCT836490.404747794352179No Hit
NCAAGC831660.4024107289398955No Hit
TACAGT829020.40113332672697033No Hit
AACATA828280.40077526701577165No Hit
NAGAGG827570.4004317232388107No Hit
NGGACT826480.3999043109615045No Hit
NACGGC817980.3957914629274652No Hit
ACTAGC814200.39396245521350426No Hit
NAACGG810740.3922882841314129No Hit
NTCTGC800580.38737222106954955No Hit
GAGCCA797490.38587707984305764No Hit
NGTTGC794470.3844158091297871No Hit
NTGTAT791320.3828916360348196No Hit
NATCCT783440.379078783975028No Hit
NCGTGG783340.3790303975275687No Hit
NCATGA782930.3788320130929856No Hit
CAAGGA781190.37799008890719404No Hit
GTCTCA760090.36778054849328473No Hit
TGTGAT759980.3677273234010795No Hit
CCACTT751710.36372576419619657No Hit
CATGAG751500.3636241526565321No Hit
CGTGAA750880.3633241566822845No Hit
NAGCAC739360.35775003793497484No Hit
AACTCT735460.3558629664840627No Hit
ATTGAA734500.3553984565884535No Hit
CAGCCT733340.35483717379792584No Hit
NCTGCT727280.3519049550818931No Hit
NCTCGG726520.35153721808120253No Hit
NACATA723370.35001304498623503No Hit
NACAGT719250.34801952335091246No Hit
TAAGTC714350.34564858742540744No Hit
NCTAGC711030.3440421573697591No Hit
NAGCCA696090.3368132221193419No Hit
AAGGCG695620.3365858058162832No Hit
AAGCGC677790.32795850223429257No Hit
TAGAGC675690.3269423868376476No Hit
NAAGGA673850.32605207620439675No Hit
CTTAGC672820.3255536957955661No Hit
GTACTT665400.32196342139408707No Hit
TAATGA664380.3214698796300024No Hit
NTCTCA661210.31993602924554304No Hit
NGTGAT657710.318242503584468No Hit
NCACTT653900.3163989799362692No Hit
NGTGAA649730.31438126507721703No Hit
NATGAG646990.3130554764168326No Hit
NACTCT645420.3122958091917218No Hit
NTTGAA638850.3091168195936468No Hit
NAGCCT635730.307607162432917No Hit
NAAGTC619800.2998992013526528No Hit
TTCTAC613070.29664279343864286No Hit
NTACTT597290.2890074120295676No Hit
GGCTAC595230.28801065121190633No Hit
NAGGCG595230.28801065121190633No Hit
NAATGA585950.2835203888876846No Hit
NAGCGC584680.2829058810049517No Hit
NTTAGC584150.2826494328334175No Hit
NAGAGC583390.2822816958327269No Hit
GCTTCT568290.27497534226637477No Hit
TCTTCA568030.2748495375029806No Hit
TCCAGA562070.27196570523440716No Hit
ACCAGG548820.2655545009460518No Hit
GCATAA534680.25871265727530884No Hit
NTCTAC533720.25824814737969964No Hit
GCTGTC531470.2571594523118657No Hit
NCTTCT527300.25514173745281354No Hit
GAGATA515850.24960148921872533No Hit
NGCTAC514880.24913214067837025No Hit
NCTTCA494600.2393193691336271No Hit
CGTACT491260.237703261788487No Hit
NCCAGA484790.2345726586378712No Hit
GTGGTG484380.2343742742032881No Hit
GTCGAG479720.23211946575168538No Hit
NCCAGG474100.22940014740447354No Hit
NCTGTC469250.2270534047026982No Hit
TGGCAT464320.2246679528429554No Hit
NCATAA464140.22458085723752866No Hit
GTAAGA459420.2222970169174504No Hit
ACTTGA451610.2185180353708802No Hit
NAGATA450100.21778740001424496No Hit
NGTACT439000.21241650434626427No Hit
GCTCAA432100.20907783947157355No Hit
GATCAG426060.2061552980450327No Hit
GGACTA422710.20453435205514664No Hit
GGTAAT421770.20407951944902936No Hit
NTCGAG416790.2016698743655569No Hit
NTGGTG416090.2013311692333419No Hit
CGAATT415760.20117149395672626No Hit
GCGCAG403490.19523447685347192No Hit
CTATGC402820.1949102876554947No Hit
NTAAGA400910.19398610650902234No Hit
NGGCAT397710.1924377401903252No Hit
NCTTGA394430.1908506647136606No Hit
CCAGCG391260.18931681432920125No Hit
TCATGT384980.18627814542875812No Hit
NCTCAA378000.18290077139609998No Hit
TAGCTT377700.18275561205372212No Hit
GTTACA374050.1809895067214582No Hit
NGTAAT369930.17899598508613562No Hit
NGACTA368370.17824115650577077No Hit
NATCAG368200.17815889954508998No Hit
GGCCGT366650.17740890960947106No Hit
CCGGAA366600.1773847163857414No Hit
CTGAGG364680.17645569659452312No Hit
CAGATC359080.1737460555368031No Hit
NGAATT358250.17334444802289103No Hit
TGTGTG357930.17318961139102135No Hit
GACACG357520.17299122695643826No Hit
CACATT354240.1714041514797737No Hit
NTATGC353410.17100254396586162No Hit
NCGCAG344070.16648324977316434No Hit
GCCATA341580.1652784272314281No Hit
NAGCTT335870.16251556108150292No Hit
NCAGCG335480.1623268539364117No Hit
NCATGT333660.1614462205926527No Hit
CTGGAG333250.16124783615806962No Hit
NTTACA326740.15809787842847012No Hit
NGCCGT317620.15368503442018325No Hit
AGTTAG317430.1535931001700106No Hit
AAGTTG317360.15355922965678911No Hit
NCGGAA316830.15330278148525492No Hit
NTGAGG315530.1526737576682842No Hit
NAGATC313730.15180280161401705No Hit
NACACG311410.15068023603296163No Hit
NACATT310680.15032701496650883No Hit
GACCGA310430.15020604884786062No Hit
NGTGTG307950.14900606495087035No Hit
NCCATA299650.14498998981174963No Hit
GTTGGC297680.1440367767968017No Hit
ACGTAA296000.1432238844794857No Hit
CCGTCG293540.14203357787198725No Hit
TATGGT292690.14162229306858332No Hit
NTGGAG286840.1387916858922151No Hit
GAGTGA277770.13440303510765791No Hit
NAGTTG277370.13420948931782076No Hit
NGTTAG275200.13315950340795427No Hit
AATGTA271970.13159662115501933No Hit
NACCGA266990.12918697607154692No Hit
CAGTCC258250.12495800056360534No Hit
NTTGGC257660.12467252052359556No Hit
NCGTCG257400.12454671576020142No Hit
NATGGT254780.12327899083676812No Hit
NCGTAA253230.12252900090114921No Hit
AGTAGG250210.12106773018787877No Hit
GTACGC248160.12007580801496343No Hit
GACCAT247000.1195145252244357No Hit
CATTAA243340.11774358124742583No Hit
NAGTGA242160.11717262116740627No Hit
NATGTA238830.11556135246701205No Hit
CACCTA234210.11332589859439304No Hit
CTCGTC233360.11291461379098913No Hit
TTCTGT228260.11044690497056554No Hit
TTAGCG222660.10773726391284555No Hit
NAGTCC222100.10746629980707355No Hit
NACCAT219550.10623244539686177No Hit
NTACGC217510.10524536186869234No Hit
NGTAGG217130.10506149336834707No Hit
ATACCT215840.10443730819612226No Hit
GACTTG214590.1038324776028812No Hit
NATTAA213040.10308248766726227No Hit
NACCTA209900.10156315321704071No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)