FastQCFastQC Report
Mon 4 Sep 2023
EGAF00001836000

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836000
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences393371368
Sequences flagged as poor quality0
Sequence length6
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCGC52599501.3371461239649753No Hit
TGTGAT49452051.2571339457527575No Hit
GGCTAC49030511.2464178633356966No Hit
CCACTT48210701.225577251468897No Hit
GAAGAT44872141.1407068142285333No Hit
CAGGAC40899551.0397185287771122No Hit
CTTAGC40637281.0330512921316632No Hit
AACATA40427071.0277074868346798No Hit
TTGAAT40365491.026142044989914No Hit
CATGAG40036281.0177731084891772No Hit
AACCTT37234970.9465602488892888No Hit
CCGTCG35899560.912612429890932No Hit
TAGCTT35451160.9012135321450239No Hit
CGTACT35405110.900042882632983No Hit
GCACAT35376420.8993135463789017No Hit
CGAGCC35174210.8941731112468765No Hit
GCCATA35115380.8926775778963151No Hit
GAGATA34654410.8809591347787162No Hit
GCTGTC34302280.8720075427553742No Hit
GTGGTG33773350.8585614700864552No Hit
TCTTCA33480870.8511262568555827No Hit
ACACGA33306050.8466821103258334No Hit
CGTTGC33219790.8444892715221715No Hit
GAGAAG32837070.8347600428305701No Hit
TTCTAC32741150.8323216345527212No Hit
CATACG32600970.8287580808372408No Hit
TCAATG32351310.8224114064143072No Hit
GTCGAG32332880.8219428923967848No Hit
ACTTGA32170360.8178114274956585No Hit
TCCAGA32036550.814409807274026No Hit
AGTTAG32014060.8138380828977874No Hit
GGCCGT31632930.8041492740264716No Hit
GAGTCG31632380.8041352923276308No Hit
TTACGT31588210.8030124348043552No Hit
ACGAAC31264780.7947904332478005No Hit
CAGATC31126130.7912657740763684No Hit
AGGACT30949200.7867679886656112No Hit
TCGTGG30773290.7822961329508863No Hit
GATCAG30706600.7806007884132533No Hit
TCCACG30532070.7761640140519835No Hit
GTAAGA30518760.7758256569400344No Hit
TGCATA30510170.7756072882254104No Hit
GCGCAG30485440.7749786202029834No Hit
ACCAGG30309680.7705105776788513No Hit
CGAATT30036620.763569045523415No Hit
GGACTA29329250.745586801325103No Hit
AACTAG29254080.7436758844126143No Hit
GGTAAT29117640.740207406249252No Hit
TGATAA28980150.7367122357517388No Hit
CTGTTC28944840.7358146106861544No Hit
TACGGC28520640.7250309076892449No Hit
ATCCGG28002020.7118469283204161No Hit
GCTCAA27760020.7056949808304299No Hit
TTAGTA27723360.7047630370495088No Hit
CAGTCC27236650.6923902504261571No Hit
CCTGCT27171150.6907251572005617No Hit
ATTCAT26836070.6822069978412867No Hit
CCAGCG26504910.6737884898628412No Hit
AAGAGG26470400.6729112018137527No Hit
CCTCGG25615770.6511854213039725No Hit
CTGGAG25593520.6506197980326824No Hit
GAGCAC25536290.6491649387151126No Hit
CTGTAT25423020.6462854713920104No Hit
ACTAAT24919170.6334769641902357No Hit
TACAGT24826140.6311120233844778No Hit
CCGGAA24599340.6253464792079122No Hit
CAACGG24482930.622387189095064No Hit
CACATT24362750.6193320607919791No Hit
ACTAGC24135680.6135596528723464No Hit
CCATGA23775910.6044138423414691No Hit
GTACTT23709840.6027342589916204No Hit
ACAGCC23482730.5969608342211628No Hit
CAGCCT23415540.5952527790482198No Hit
CTGAGG23387110.5945300523244996No Hit
GGTACC23289570.5920504615882466No Hit
GTCTGC23020290.585205021835753No Hit
CAAGGA22920950.5826796728123843No Hit
GTCTCA22897780.582090661972124No Hit
GACTTG22844870.5807456225436316No Hit
TCATGT22488570.571688023821805No Hit
AACTCT22212420.5646679399401534No Hit
CGTGAA21694530.551502518098877No Hit
TAGAGC21621860.5496551543629378No Hit
AAGGCG20823440.5293583034746951No Hit
CGAAGC20584210.523276772904326No Hit
GCTTCT19899640.5058741336761449No Hit
GTTGGC18743640.47648714483968235No Hit
GACACG18687680.47506457053579965No Hit
CTATTA15548800.3952702526127931No Hit
ATTGAA14871250.378046070704363No Hit
AAGCAT14702190.37374835069338347No Hit
TGTGTG14527240.3693008993984534No Hit
GCATAA14388870.36578335818279484No Hit
TCGGAC13924220.3539713647893153No Hit
GGATCG13596140.3456311543243788No Hit
TCAAGC12711320.32313790565458744No Hit
CGAGAG12660510.32184625089439656No Hit
GAACCG12387700.3149110740566151No Hit
TATTGG12283040.31225048387354926No Hit
TGACCA12229950.31090086861634525No Hit
GCCAAT11823380.3005653426204624No Hit
GATCCT11731550.29823090733944824No Hit
CGATGT11521760.2928977789761252No Hit
AGTGCA11047830.2808498761912941No Hit
GACCGA10718370.2724745843729023No Hit
AAGTTG10384150.26397828730636036No Hit
TAAGTC10229250.2600405324873568No Hit
GTTACA10193120.2591220619798643No Hit
GAGCCA9925980.252331023746497No Hit
AGGTGT9863740.25074880386312204No Hit
AATCGG9675580.24596553758330472No Hit
TGCGAC9604050.24414715409587207No Hit
TTCCAT9522380.24207099892435488No Hit
ACAGTG9255410.2352842823069929No Hit
TATGGT9000020.22879194400340802No Hit
AATGTA8986680.22845282425334018No Hit
TAATGA8906150.2264056493303295No Hit
TACTCG8689930.22090906219692125No Hit
TGGCAT8685110.22078653167253393No Hit
GGATTC8647700.21983552193864808No Hit
AGAGGT8636350.21954699051711357No Hit
GTACGC8588340.21832651531465808No Hit
CGACAA8574050.21796324535750147No Hit
ACGTAA8354830.21239039441223387No Hit
CTCGTC8289750.2107359781202988No Hit
GAGTGA7874560.20018132077167344No Hit
TTAGCG7740520.19677385365779848No Hit
TACGAA7643830.19431587100157224No Hit
CGTCTC7585690.19283787832773838No Hit
CATTAA7522550.19123277930080565No Hit
ATCCAC7512270.19097144863883433No Hit
TTAGGC7421310.1886591298632594No Hit
TATTCT7361340.18713461626419134No Hit
GGCAGG7135940.18140466186649354No Hit
AGGCTT7108170.1806987131813823No Hit
TTCGTT7058570.17943781815864138No Hit
AGTTCC6990580.17770942596920272No Hit
CACCTA6970120.17718930677232209No Hit
AGTAGG6964900.17705660773968684No Hit
ACCGCT6943230.17650572880535625No Hit
CTCCGC6826340.17353423648261052No Hit
CTTCAC6822230.17342975506036323No Hit
ATGTGA6815430.17325689042014872No Hit
GACCAT6803900.17296378316990269No Hit
TCTCGC6644100.1689014641248623No Hit
CGTCAG6620000.16828881150292566No Hit
CTATGC6342570.16123618839488082No Hit
ACCTGC6333320.1610010416416479No Hit
TTCTGT6236420.15853772051859147No Hit
TCTCTG6226560.15828706679028048No Hit
TCCTCC5831190.14823625902533913No Hit
ATTGCT5788290.1471456865157507No Hit
ACGCTA5780920.14695833175128292No Hit
CGCTTG5765420.14656430205667637No Hit
GACTAA5738810.1458878420454841No Hit
CTACCG5682800.1444639966780704No Hit
AGATGC5626800.14304040552336286No Hit
ATACCT5423390.13786946486659396No Hit
AGTCAA5241590.13324787786791845No Hit
TGCGGA5182390.13174293864722764No Hit
AATTGC5143530.1307550680709431No Hit
GCGTAT5118650.13012258685792302No Hit
TGACAC5038970.12809702001493917No Hit
GGAGGC4953430.12592248452612342No Hit
ATTCGA4844530.12315410815562967No Hit
AGAACG4813120.12235562604546246No Hit
AGGTCA4700430.11949090305931975No Hit
CCTGAC4599000.11691242358035575No Hit
CCGCTC4517210.11483321785636418No Hit
CATAAC4481430.11392364479358853No Hit
CAAGCT4451400.11316024403687662No Hit
TTCACA4400470.11186553872421137No Hit
GCCTGG4374440.11120382304997856No Hit
CTAGTT4342040.11038017388189778No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)