FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001836002

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836002
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22432528
Sequences flagged as poor quality0
Sequence length6
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCAA8298543.699333396574831No Hit
TCTTCA6919993.0848016772786377No Hit
CGTACT5848612.6072005794442785No Hit
CCGGAA5370512.3940725717582967No Hit
CATACG4634542.065990957416837No Hit
GTCTGC4211611.877456700377238No Hit
TGATAA4154111.8518242794570456No Hit
CCAGCG3694471.6469253933395291No Hit
ATGTGA3234611.4419284353506656No Hit
GCGCAG3209281.4306367967087794No Hit
CTACCG3106471.3848060281034755No Hit
AACCTT3079461.372765476989486No Hit
AGAACG2977181.3271709724378813No Hit
CTGTAT2727461.2158504828345695No Hit
ATTGAA2641091.1773483577062738No Hit
TGCGAC2528921.127345076756396No Hit
AGGTCA2505331.116829097460616No Hit
ACTAAT2445691.090242704700959No Hit
CTGTTC2423271.0802482894482512No Hit
ATCCGG2411471.0749880708941943No Hit
TATAAG2340941.0435471205028697No Hit
TTCACA2314311.0316759662575703No Hit
CTGAGG2271571.0126232763422829No Hit
ACGAAC2258411.0067567953108094No Hit
CACATT2218720.9890637381573758No Hit
TTCTGT2213230.9866163991860392No Hit
TTCTAC2187800.9752801824208133No Hit
ATCAGC2179330.9715044153739605No Hit
TCCAGA2150280.9585544705438459No Hit
ATTCAT2140300.9541055738345674No Hit
TGCATA1937920.8638883678201583No Hit
ATACCT1876420.8364728219663874No Hit
CGTGAA1876020.8362945094730295No Hit
GCACAT1827540.814683035278057No Hit
CGAATT1812800.8081122198978198No Hit
ACAGCC1767200.7877845956550238No Hit
GGACTA1679780.7488144002316636No Hit
AAGCAT1664290.7419092489263804No Hit
TAAGTC1645430.7335018148645573No Hit
GGTAAT1616920.720792591900476No Hit
TGGCAT1614510.7197182591279948No Hit
CTTAGC1612490.7188177810365377No Hit
TTGAAT1576420.7027384519479927No Hit
GAGAAG1565940.6980666646220167No Hit
GTAAGA1543690.6881480321789858No Hit
CAGCCT1535110.6843232291964597No Hit
TGTGAT1524230.6794731293771259No Hit
CTATGC1517880.6766424185450699No Hit
TAATGA1508320.672380749953817No Hit
GACCAT1507370.6719572577820921No Hit
CCACTT1484810.6619004331567089No Hit
TTACGT1468730.6547322709237229No Hit
AAGCGC1463240.6522849319523862No Hit
GAGATA1447430.645237130652417No Hit
GTGGTG1395920.6222749393202585No Hit
TCGTGG1374920.6129135334189709No Hit
TTAGTA1368770.6101719788335938No Hit
CCTGCT1345700.5998878057791792No Hit
CTATTA1323780.5901162811431685No Hit
CACCTA1310380.5841428126156802No Hit
CTAGTT1297700.5784903065762361No Hit
CTGGAG1212970.5407192626707075No Hit
CCATGA1207470.5382674658870369No Hit
TCTCTG1197580.5338586894887638No Hit
GAAGAT1168520.5209042868463154No Hit
CAAGCT1153970.5144181699004231No Hit
CATAAC1121090.4997608829464071No Hit
TCATGT1120900.4996761845120622No Hit
GGCCGT1092100.4868376849902962No Hit
TCAAGC1090440.4860976881428611No Hit
TATTCT1075470.4794243430789432No Hit
ACACGA1031680.45990358286859157No Hit
CATGAG1002830.44704279428515586No Hit
AACTAG999180.4454156927832654No Hit
CCTCGG965950.43060238239756127No Hit
TAGAGC943650.42066146089286055No Hit
AAGGCG932010.4154725673361469No Hit
AAGAGG924280.41202667840200624No Hit
TCCACG897590.4001287772827031No Hit
TACGGC895360.399134685132233No Hit
TACTCG876810.39086544325276223No Hit
CGCTTG862530.38449968723988664No Hit
CCTTAT858010.38248475606494287No Hit
ATTGCT852100.3798501889755805No Hit
AGGCTT851120.3794133233668537No Hit
GTCGAG840430.3746479219818649No Hit
GTACGC830970.3704308315139515No Hit
GGAGGC825810.36813060034963513No Hit
AATCGG822460.3666372332177631No Hit
CGAGCC812390.36214821619747894No Hit
GATCAG794910.35435596023774046No Hit
CAACGG793450.3537051196369843No Hit
ACCGCT772670.3444417856070435No Hit
GCTGTC756390.3371844671273786No Hit
AGATGC742750.33110401110387555No Hit
ACCTGC737570.3287948643148913No Hit
GGTACC729320.3251171691393855No Hit
GGATTC728200.3246178941579834No Hit
AGTCAA712090.3174363584879957No Hit
CGTCAG711370.31711539599995153No Hit
ACTTGA699010.31160553995519363No Hit
CAGATC695420.31000518532730686No Hit
ATTCGA687760.3065905010795038No Hit
GCATAA674010.3004610091203274No Hit
GAGTCG669910.2986333060634094No Hit
GGCTAC662760.29544597024463765No Hit
AACATA662460.29531223587461924No Hit
GACACG658110.2933730875093525No Hit
ACGCTA648660.28916045485377306No Hit
GAATAC647920.288830576741061No Hit
ATCCAC627980.2799416989471717No Hit
GATTCA624200.2782566458849399No Hit
ACCAGG618690.2758003912889354No Hit
TAGCTT614230.2738122069879953No Hit
CAGGAC607950.2710127008422769No Hit
AGTAGG607560.270838846161253No Hit
GTCTCA588270.2622397261690702No Hit
AATTGC582230.25954720751936655No Hit
AGGTGT581280.2591237153476416No Hit
CGTCTC562540.25076977503382586No Hit
GAGCAC558900.24914713134426936No Hit
GTTACA550790.24553184554143873No Hit
GACTAA547450.24404293622190062No Hit
CAAGGA545150.24301763938509294No Hit
GCCTGG531010.23671429274489258No Hit
CTCCGC511490.22801264306902908No Hit
TCTCGC511010.2277986680769996No Hit
AGAGGT505580.22537807597966664No Hit
TATGGT486250.2167611247381481No Hit
ACGTAA477360.21279812957326968No Hit
ATGGCC476820.21255740770723658No Hit
GTACTT476260.21230777021653555No Hit
GTTGGC475880.2121383733478456No Hit
ACTAGC471950.21038645310060464No Hit
GAGCCA469540.2093121203281235No Hit
TTAGGC466650.208023812563613No Hit
AGGACT462620.20622731419303256No Hit
TACAGT456460.20348130179532153No Hit
TCAATG454630.2026655221382093No Hit
GCCAAT451450.2012479378160143No Hit
CGTTGC435400.1940931490200302No Hit
CGAGAG417600.18615824306560544No Hit
GGCCAG415760.1853380055961593No Hit
CAGTAG409990.18276584787947217No Hit
ATGCCG409630.1826053666354501No Hit
GGATCG407960.18186091197568102No Hit
AACTCT406000.1809871807582275No Hit
CGATGT398410.17760370119676214No Hit
GATCCT396610.17680129497665176No Hit
CTCGTC395740.17641346530359842No Hit
GCTTCT384630.17146083580058386No Hit
CATTAA371960.16581278757347365No Hit
TATTGG363950.1622420798939825No Hit
ACAGTG362150.16143967367387216No Hit
TGCGGA359760.16037425652605894No Hit
GAGTGA353250.15747222069665978No Hit
TGTGTG338150.15074092407240058No Hit
AGACGG334640.14917623194318536No Hit
GACCGA319890.1426009587506143No Hit
TCCTCC302320.1347685824798703No Hit
GGCGTT295160.13157678884876461No Hit
AAGTTG272730.12157791578372264No Hit
AGCCTA270570.12061502831959019No Hit
CCGCTC270520.12059273925792047No Hit
TGACCA268080.11950503304843751No Hit
AACAAT257440.11476192072511844No Hit
AATGTA251990.11233241300311761No Hit
GGTGGT245350.10937242561337715No Hit
CCGACT241290.10756255380579487No Hit
GCGTAT241210.10752689130712331No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)