FastQCFastQC Report
Thu 14 Sep 2023
EGAF00001836014

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836014
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences418419970
Sequences flagged as poor quality0
Sequence length6
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACAT48605471.1616431691823887No Hit
CCGTCG48298141.1542981564670538No Hit
TACAGT48101381.1495957040482556No Hit
GGCTAC48019431.14763714552152No Hit
CATACG45549201.0886000493714485No Hit
CCTCGG45520151.0879057708454976No Hit
AAGCGC44939051.0740178103831899No Hit
GAAGAT43801081.046820972717913No Hit
GTACTT43353631.0361271714636375No Hit
ACCAGG42888771.0250172810824494No Hit
CAGTCC39854660.9525037727047301No Hit
GACACG39851900.9524378102699066No Hit
GAGTCG39546300.9451341435735011No Hit
TTAGTA37872970.9051425055070866No Hit
TTACGT37677510.9004711223510675No Hit
AACCTT37575070.8980228644440656No Hit
CCATGA34747240.8304393310864202No Hit
GTCTCA34292250.819565328108025No Hit
CAGATC34026810.8132214626371681No Hit
GATCAG33910930.810451996351895No Hit
AACATA33518460.8010721859188509No Hit
ACTAAT32598020.7790741918938524No Hit
TAGAGC32506310.7768823749019436No Hit
GAGATA32473820.7761058823267923No Hit
ACAGCC32455770.7756744975628195No Hit
TCGTGG32177750.7690299772259914No Hit
CAGGAC30874840.7378911670970199No Hit
TCAATG30724650.7343017112687045No Hit
ACACGA30155170.720691462216777No Hit
CGAGCC29541080.7060150594628645No Hit
CAACGG28445070.679821042002369No Hit
GTAAGA28213750.6742926251823019No Hit
TGTGTG28106920.6717394487648379No Hit
ATCCGG28027420.6698394438487245No Hit
GCCATA27875710.6662136608823904No Hit
TTGAAT27153620.6489561193745126No Hit
GAATAC26622040.6362516588297638No Hit
CAGTAG26236930.6270477482229159No Hit
CTGTTC25820180.6170876595588878No Hit
GGTAAT25685530.6138696009179485No Hit
CGAATT25618800.6122747917600587No Hit
CAGCCT25285190.6043017019479258No Hit
GCTGTC25145370.6009600832388569No Hit
GTCTGC25059390.5989052099975056No Hit
GCTTCT25035700.5983390324319368No Hit
ACGAAC24929390.5957982837195844No Hit
CTTAGC24913860.5954271255265373No Hit
AGGACT24690840.590097073999599No Hit
AAGAGG24681470.5898731363132595No Hit
GGCCGT24582480.5875073314497872No Hit
GCGTAT24474310.5849221297922276No Hit
CACATT24382080.582717885095207No Hit
AGTTAG24048780.5747522041072752No Hit
CGAAGC23716130.566802057750733No Hit
AACTAG23666040.5656049351564172No Hit
CCGCTC23635940.5648855622259139No Hit
AGTTCC23228120.555138895497746No Hit
CTGGAG23185330.5541162387636517No Hit
TGATAA23067830.5513080553970692No Hit
ATTGAA22903280.547375403712208No Hit
GCGGCT22260020.5320018545003958No Hit
CGTTGC22236860.5314483436342677No Hit
GGTACC22024750.5263790349203457No Hit
TCGGAC22016600.5261842545421529No Hit
ACTTGA21704120.5187161597473466No Hit
GAGCAC21558540.5152368803047331No Hit
GCCAAT21422160.5119774756448646No Hit
TGCATA21071370.5035937935753879No Hit
GTTGGC20700530.4947309278761241No Hit
CCTGCT20661020.4937866612819651No Hit
TGCGGA20501270.48996872687505805No Hit
TAGCTT20441560.48854169173617595No Hit
TGGCAT20421920.4880723068738808No Hit
CAAGGA20219210.48322765282928537No Hit
CTGTAT20176960.482217901788961No Hit
TTCGTT19734140.4716347549090451No Hit
AGATGC19677580.4702830029838203No Hit
CCAGCG19483740.4656503369091107No Hit
CATAAC19262780.46036951821396094No Hit
ATTCAT19056980.45545101492168266No Hit
CGACAA18784230.4489324445962749No Hit
AACTCT18717050.4473268806935768No Hit
CTGAGG18605620.44466376688474024No Hit
TGACCA18438320.44066539175938474No Hit
GTCGAG18424010.44032339087448424No Hit
CATGAG18299380.4373448045512741No Hit
CCTGAC18296920.4372860119463228No Hit
GATCCT18045070.4312669397686731No Hit
TCCACG17961960.42928065789976516No Hit
TTCTAC17455190.4171691422854411No Hit
CGTGAA17362920.4149639416111042No Hit
CGTACT16790390.4012807992888103No Hit
GACTTG16750300.40032267102356517No Hit
GGACTA16484940.3939807175073408No Hit
TCCTCC16341160.3905444570439599No Hit
GGCAGG16192680.3869958692459158No Hit
CTTCAC16147540.3859170488444899No Hit
GTGGTG16065300.3839515594822111No Hit
GAGAAG15833300.37840689104776715No Hit
TCATGT15773190.3769702961357222No Hit
CGATGT15575750.3722515921025471No Hit
GACTAA15274810.3650592967634886No Hit
GAACCG14996970.3584190783245838No Hit
TATAAG14633240.34972613759328935No Hit
CATTAA14593070.3487660973734117No Hit
ACAGTG14569890.34821210851862544No Hit
GCATAA14488030.34625570094085134No Hit
CCACTT14425890.344770590180005No Hit
CAAGCT14138390.3378995032192178No Hit
CGAGAG13985500.33424551892205334No Hit
TGACAC13900230.3322076142780661No Hit
CGTCTC13805700.32994840088535926No Hit
TGTGAT13654930.32634508338595786No Hit
TATTGG13543060.3236714538266422No Hit
GACCGA13406500.32040774726885046No Hit
CTCCGC13375240.31966065099617497No Hit
TCTCTG13144850.3141544606487114No Hit
AGTCAA13119410.3135464590755551No Hit
AGACGG13031480.311444981940035No Hit
ACGTAA12748300.30467714052940637No Hit
AAGTTG12741560.30451605835161266No Hit
TTCCAT12609390.30135727030428305No Hit
TCCAGA12550140.29994122890453817No Hit
CTATTA12374360.2957401865881306No Hit
TATGGT11984850.28643111847649144No Hit
GGATCG11955290.285724651239758No Hit
CACCTA11852750.2832740033894654No Hit
ACGCTA11740400.28058890210235427No Hit
TAAGTC11628750.2779205304182781No Hit
TATTCT11569000.2764925393020797No Hit
GTTACA11126890.2659263610195278No Hit
TAATGA11050800.26410785316962765No Hit
ATGCCG11033950.26370514772514325No Hit
AATGTA10991970.2627018495317037No Hit
TACGGC10771930.2574430183148285No Hit
GGCGTT10557460.25231730693924576No Hit
TCTCGC10533950.25175543127160016No Hit
AGCCTA10358310.24755773487579957No Hit
AGTGCA10299350.24614862431159776No Hit
AATTGC10281330.24571795653061204No Hit
GAGTGA10164340.24292196187481202No Hit
TTAGGC10099180.2413646748265863No Hit
CCGGAA10038290.23990943835687384No Hit
AGGCTT9979490.23850415170193717No Hit
AAGCAT9875210.23601191883838624No Hit
GCGCAG9857030.2355774271481354No Hit
CTATGC9849620.23540033235029387No Hit
GGAGGC9743300.2328593446436125No Hit
GCTCAA9705310.2319514051874723No Hit
TTCTGT9629140.23013098538293952No Hit
ATGTGA9501150.2270720969651616No Hit
TCTTCA9376170.22408514584043396No Hit
ATCAGC9325820.22288180939356214No Hit
TCAAGC9307930.2224542485388544No Hit
ACCTGC9245800.22096937677233713No Hit
CGCTTG9190650.2196513230475113No Hit
AGAGGT8978640.21458440427687997No Hit
TACGAA8821890.21083816816869425No Hit
AGGTCA8804960.21043355076957726No Hit
GAGCCA8779090.20981527244027096No Hit
GGATTC8697390.207862688771762No Hit
ACTAGC8613150.20584940054366907No Hit
ATACCT8607930.2057246455038941No Hit
CTAGTT8372550.20009919698622414No Hit
CCGACT8317820.19879118102321933No Hit
TGCGAC8237050.19686082382731396No Hit
ATTCGA8019080.19165146443655642No Hit
ATTGCT7702840.18409350777401948No Hit
CGTCAG7627710.18229794337971011No Hit
GGCCAG7570540.180931612800412No Hit
TTCACA7460410.17829956825435458No Hit
AATCGG7307780.17465179780974605No Hit
GGTGGT6990320.16706468383906245No Hit
AAGGCG6867640.16413270141002115No Hit
TACTCG6841780.16351466207504387No Hit
AACAAT6616470.1581298808467483No Hit
GTACGC6495170.15523087963511875No Hit
GACCAT6395490.1528485841629404No Hit
AGGTGT5945700.14209885823566212No Hit
AGTAGG5613030.1341482338904618No Hit
CCTTAT5530500.13217581369263995No Hit
CTCGTC5304660.12677836576490362No Hit
GCCTGG5300080.12666890636218917No Hit
ACCGCT4934010.11792004095789214No Hit
GATTCA4791960.11452512651344054No Hit
CTACCG4743450.1133657650231178No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)