FastQCFastQC Report
Thu 14 Sep 2023
EGAF00001836016

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836016
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences418006959
Sequences flagged as poor quality0
Sequence length6
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAC56635631.3548968212273231No Hit
CCGGAA51525001.2326349810841308No Hit
CCACTT50038951.1970841375394423No Hit
TCCAGA46985751.1240422913629053No Hit
TTACGT39222170.9383138044838149No Hit
AACATA39112630.9356932739485804No Hit
CCTTAT38906850.9307703893991871No Hit
GTGGTG37290530.8921030905612267No Hit
CAGATC36743970.8790277101582896No Hit
CCAGCG35942420.8598521920779792No Hit
TCGTGG35779890.855963979298249No Hit
TAGCTT35178940.8415874243854395No Hit
GGTACC34758850.8315375916983239No Hit
CTATTA34659580.8291627508526719No Hit
TACGGC33766680.807801862456553No Hit
GATCAG33755660.8075382304819476No Hit
ACTAAT33743080.8072372785544941No Hit
TAGAGC33381570.7985888579429128No Hit
CAGTAG33256450.7955956063401328No Hit
GTCTCA33106010.7919966231949741No Hit
CATGAG32571210.7792025778211985No Hit
TTGAAT32426330.7757366068156775No Hit
GTCTGC31663900.7574969583221699No Hit
GCTGTC31389790.7509394119919424No Hit
GGACTA30955510.7405501112721906No Hit
GCACAT30564480.7311954823221017No Hit
AACTAG30058990.7191026214470272No Hit
GAGATA29841340.7138957703333355No Hit
CTGGAG29628800.7088111659882677No Hit
TTAGTA29613250.7084391626121229No Hit
GGTAAT29451430.7045679351955478No Hit
CAACGG29428850.7040277528011203No Hit
AGGACT29307110.7011153610961773No Hit
GAGCAC29267660.7001715969039645No Hit
ACTTGA28744000.6876440542703979No Hit
GTTGGC28220030.6751090954923552No Hit
CAAGGA27763070.664177220073506No Hit
ACACGA27559120.6592981146995689No Hit
CCTCGG27488940.6576191952823446No Hit
GCTCAA27191940.6505140504132133No Hit
TGTGAT26984110.6455421236180903No Hit
CCATGA26889050.6432679987990343No Hit
CGTACT26390650.6313447523250445No Hit
CGTTGC26263590.6283050900116713No Hit
ACCAGG26130070.6251108848166329No Hit
AACTCT25940020.6205643097917899No Hit
ATGCCG25108710.6006768418417647No Hit
TCAATG25038270.598991702432399No Hit
AAGGCG25031050.5988189780352436No Hit
GACTAA24501790.5861574663401716No Hit
CTGAGG24499810.5861100987077108No Hit
TATAAG24300310.5813374508915771No Hit
TCATGT24299220.581311374770677No Hit
GACCAT24291060.5811161627096261No Hit
CTGTAT24272260.5806664094317148No Hit
GAGTCG24239900.5798922596405865No Hit
TTAGCG24030060.5748722475216017No Hit
GTACTT24029880.5748679413731962No Hit
TGGCAT23734650.5678051402967194No Hit
GAGAAG23631420.5653355641861455No Hit
GGCCAG23402840.5598672341720512No Hit
AAGAGG23203040.5550874094419084No Hit
TGATAA23169920.5542950781352901No Hit
GCATAA22830010.5461633953323729No Hit
TCTTCA22581920.5402283266772121No Hit
GGCCGT22261750.5325688848161019No Hit
ACAGCC22247490.5322277421701967No Hit
GTAAGA22192640.5309155630588437No Hit
AACCTT21681030.518676293137981No Hit
CGAATT21656740.5180952023337009No Hit
CATACG21346450.510672120173961No Hit
CAGCCT21340680.510534084194517No Hit
GAAGAT20905390.5001206211976006No Hit
ACAGTG20850920.49881753284399266No Hit
CCTGCT20766860.4968065615386082No Hit
CTTAGC20715350.49557428540322457No Hit
GTCGAG20558960.49183296013021643No Hit
GACACG20014420.47880590428160785No Hit
CGAGCC19801940.4737227353193419No Hit
TGTGTG19586870.4685776056661296No Hit
TTCTGT19565330.4680623032402674No Hit
CTGTTC19386360.4637807955728316No Hit
CGTGAA19347220.46284444752509496No Hit
GGTGGT19302060.4617640827362398No Hit
GCTTCT19184220.4589449909134168No Hit
ATTGAA19166170.45851317992052854No Hit
TACAGT19058170.45592949087720813No Hit
TAAGTC18944050.4531993927880995No Hit
AAGCGC18887830.45185443910277107No Hit
GCGGCT18836680.4506307752641985No Hit
TGCGGA18640100.4459279827444212No Hit
GCGCAG18578910.44446413151700664No Hit
CCGCTC18452590.4414421722581896No Hit
CGATGT17792960.425661812965176No Hit
CACATT17674380.422825018087797No Hit
GTTACA17314480.4142151135813985No Hit
TCTCTG17277440.41332900393172645No Hit
ACTAGC17148150.4102359932242181No Hit
GAATAC17045820.40778794785567196No Hit
TGCATA16988730.40642218111971673No Hit
ATGGCC16616990.3975290277404209No Hit
CTCCGC15812710.37828819974262673No Hit
TTCTAC15788610.3777116543172192No Hit
AGTTCC15617290.3736131579570186No Hit
ATCCGG15504130.37090602599273953No Hit
CTACCG15468410.37005149476470794No Hit
ATACCT15280320.36555180891139183No Hit
TCCACG14961630.3579277731593937No Hit
TCAAGC14911480.35672803236751854No Hit
GCGTAT14649540.35046162951559856No Hit
TATGGT14540350.3478494720467082No Hit
CAGGAC14399000.34446794939602904No Hit
TGACAC14387800.3442000112730181No Hit
ATTCAT14127930.33798312912776174No Hit
GCCAAT13980830.33446404895857246No Hit
CACCTA13685120.3273897648196809No Hit
TTAGGC13499870.3229580204189854No Hit
GAGCCA13398790.3205398788588111No Hit
TGCGAC13306470.318331303187706No Hit
TGACCA13258480.31718323617669725No Hit
AGAACG13008550.31120414911561317No Hit
CATTAA12889870.30836496193356433No Hit
ACGAAC12726230.30445019457199995No Hit
CGTCAG12621160.3019366000555029No Hit
AGACGG12284130.29387381562707426No Hit
AAGTTG12018330.28751506981490227No Hit
GGCGTT11852920.28355795866068345No Hit
GAGTGA11751180.28112402788968877No Hit
ATGTGA11727310.2805529847650216No Hit
ATCAGC11712770.2802051436660412No Hit
TCCTCC11676140.27932884246551504No Hit
CTAGTT11654370.2788080377389124No Hit
GTACGC11553310.276390374639672No Hit
AGTAGG11478160.2745925576803615No Hit
AATCGG11374780.2721193931127831No Hit
ATTGCT11209660.2681692196421065No Hit
GATCCT10815310.2587351661769822No Hit
CTTCAC10778910.25786436727719647No Hit
TCTCGC10696530.2558935866902637No Hit
GGCAGG10319570.24687555500720743No Hit
CAAGCT10258450.24541337839306165No Hit
GGAGGC10187860.24372465052669134No Hit
AAGCAT10059740.240659629783819No Hit
AACAAT9996570.23914841092394348No Hit
TATTGG9864170.2359809995412062No Hit
TAATGA9801350.23447815374767483No Hit
GATTCA9692770.23188058933726985No Hit
ATTCGA9657290.23103179964044568No Hit
CCTGAC9641200.23064687781908436No Hit
TTCACA9409810.2251113240437703No Hit
GGATCG9346590.22359890903155993No Hit
CGAGAG9295730.22238218287652958No Hit
CTATGC9274410.22187214352094076No Hit
CATAAC9244500.22115660519422117No Hit
GACCGA9168780.21934515209829317No Hit
AGGTCA9156280.21904611401457552No Hit
CGTCTC9112490.21799852379969586No Hit
CCGTCG9109480.21792651542913666No Hit
TATTCT9003940.21540167708069186No Hit
ACCGCT8794300.2103864495710465No Hit
AATTGC8776940.20997114548037943No Hit
AATGTA8482660.20293107129826515No Hit
CTCGTC8261460.1976392933687977No Hit
TACTCG8224300.1967503129535219No Hit
AGGTGT8059740.19281353638899587No Hit
ACGCTA7934310.18981286864173955No Hit
TTCCAT7806440.18675382866054152No Hit
TTCGTT7514790.17977667209124143No Hit
CGCTTG7495790.17932213420399062No Hit
AGATGC7433270.17782646532446844No Hit
AGGCTT7335880.17549659980660753No Hit
GACTTG7178440.17173015533456706No Hit
AGCCTA7146700.1709708378323912No Hit
ACCTGC6804300.16277958664319747No Hit
GGATTC6646480.15900405141341198No Hit
ACGTAA6622660.15843420444107964No Hit
AGAGGT6591590.15769091538019106No Hit
CGAAGC6015560.14391052279108107No Hit
AGTTAG6007210.1437107653511577No Hit
CCGACT5935830.14200313827789648No Hit
ATCCAC5929440.1418502700095No Hit
CGACAA5599220.13395040152908078No Hit
GCCTGG5535880.1324351157512667No Hit
AGTCAA5447990.13033251917703123No Hit
CAGTCC4976920.11906308956928155No Hit
AGTGCA4859450.11625284927373662No Hit
GCCATA4277590.10233298532238072No Hit
TCGGAC4247270.10160763854651521No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)