FastQCFastQC Report
Thu 14 Sep 2023
EGAF00001836018

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836018
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences416135500
Sequences flagged as poor quality0
Sequence length6
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTCA58278091.4004594657269087No Hit
GGCTAC51955641.2485269821969047No Hit
ACCAGG45576871.0952410933457972No Hit
CGTACT41664841.001232531230813No Hit
CAGATC41174910.9894592025914636No Hit
CCACTT40325260.9690415741987887No Hit
GGACTA40230700.966769237423868No Hit
TAGCTT38215960.9183537573698951No Hit
GCACAT37460650.9002031790126052No Hit
TCGTGG37405980.8988894242380187No Hit
CGAATT36556930.8784862142258952No Hit
GAGATA36324170.8728928437972728No Hit
CCTGCT36101950.8675527562536722No Hit
TTCTAC35429400.851390953187123No Hit
ACTTGA34462720.8281610196678726No Hit
GGCCGT34028320.817722112148567No Hit
TGTGAT33622000.8079579848390729No Hit
TGTGTG32257700.7751729905283256No Hit
GGTACC31944690.7676511617009363No Hit
CTGAGG30905750.7426847745505971No Hit
CACATT30857770.74153178471916No Hit
GTGGTG30822690.7406887900695807No Hit
CTATTA30105980.7234657941944391No Hit
TTGAAT29878510.7179995458210126No Hit
GAGCAC29378330.7059799031805746No Hit
TCATGT29103860.6993842149972785No Hit
GTAAGA28289800.6798218368776516No Hit
AGGTCA28261900.6791513821820057No Hit
GATCAG27843550.6690981663424533No Hit
TTAGCG27527930.6615136175596651No Hit
TCCAGA27404820.6585552061768342No Hit
AAGCGC27357210.6574111076800705No Hit
ACACGA27168550.6528774882219854No Hit
GACTAA27079450.6507363587100836No Hit
GCGCAG25992540.6246172220346498No Hit
CGATGT25675440.6169971079131676No Hit
TTAGTA25632810.6159726819749818No Hit
CATAAC25506430.6129356904181451No Hit
ACAGTG24894650.5982342289951229No Hit
GAAGAT24116800.5795420001417808No Hit
TTACGT23671540.5688421199344925No Hit
GGTAAT23501310.5647513850656818No Hit
TGGCAT23271550.559230106539817No Hit
AGGACT23042270.5537203627183934No Hit
TTAGGC22754760.5468113150644441No Hit
CCAGCG22637530.5439942038110183No Hit
TGATAA22540610.5416651547392616No Hit
CCGCTC21953290.5275514826300568No Hit
ACAGCC21881150.5258179126750782No Hit
CTGTAT21464350.5158019443186174No Hit
CGTTGC21284770.5114865230195453No Hit
CGCTTG21208150.5096452958231152No Hit
CAACGG21176370.5088816022665694No Hit
AAGAGG21118620.507493833138485No Hit
CTGGAG20993270.5044815931349285No Hit
TTCTGT20669970.4967124890810806No Hit
GCTCAA20583230.49462807186601476No Hit
AGTTCC20522950.49317950523327136No Hit
CCATGA20441240.4912159621084959No Hit
GCCAAT20426000.4908497352424871No Hit
TGCGAC20381330.48977628681042595No Hit
CAAGCT20184360.4850429727817021No Hit
AGAACG19978920.4801061192808593No Hit
GCTTCT19659240.4724240061230056No Hit
CGAGCC19556500.46995509875989916No Hit
AACATA19550330.4698068297465609No Hit
TCAATG19542810.4696261193769818No Hit
CTTAGC19520580.46909191837754766No Hit
ACGAAC19469000.46785241826280144No Hit
CTACCG19418850.4666472819550363No Hit
TAGAGC19243060.4624229367597814No Hit
TAATGA19066740.45818585532837264No Hit
CCGGAA18903770.4542695828642353No Hit
ACTAGC18725500.4499856416960341No Hit
ATTCAT18706910.4495389122052793No Hit
GTCTGC18397170.44209566355189595No Hit
CGTCTC18183400.43695863486772935No Hit
GTCTCA18055480.4338846361341438No Hit
ATTGCT18037640.43345592961907836No Hit
TGACCA18027540.4332132202131277No Hit
AACTAG18017000.4329599373281059No Hit
ACTAAT17895970.4300515096645203No Hit
CATACG17863920.4292813278367263No Hit
ATGTGA17573270.422296823991224No Hit
AACCTT17535200.42138197774522956No Hit
CCGTCG17512650.4208400869428347No Hit
GACACG17503930.4206305398121525No Hit
CTGTTC17501180.420564455567958No Hit
TACGGC17497990.4204877978446924No Hit
CAAGGA17178500.4128102505073467No Hit
GCGTAT16546860.39763154068806916No Hit
CAGTCC16534360.39733115775991235No Hit
AGGCTT16481200.39605368924304707No Hit
GAGAAG16369580.3933713898477779No Hit
GTCGAG16208450.38949933375066537No Hit
CAGGAC16195580.3891900594878351No Hit
TATTGG16009910.38472829162616506No Hit
ATACCT15901470.382122409647819No Hit
GATTCA15883160.3816824087346549No Hit
GTACTT15756080.3786285957338415No Hit
ACCTGC15676670.37672032306784686No Hit
CACCTA15634170.37569902111211373No Hit
AACTCT15553130.37375157851228746No Hit
GCCTGG15519070.37293309510964573No Hit
ATGGCC15505330.3726029141950158No Hit
AGATGC15313290.3679880711931571No Hit
AATTGC15262940.3667781287585414No Hit
TACAGT15063750.3619914667217769No Hit
TGCGGA15053880.3617542843617043No Hit
GAGTCG15047840.3616091393308189No Hit
AAGCAT14883310.35765537907724765No Hit
CTCCGC14607140.3510188388157223No Hit
CGAAGC14503740.3485340712340091No Hit
CCTGAC14478020.3479160033210337No Hit
TGCATA14454750.3473568104619769No Hit
GACCGA14364630.34519116970313757No Hit
GCCATA14028790.3371207214957628No Hit
AGTTAG13951460.33526243254901344No Hit
ATCCGG13940080.3349889639312195No Hit
CTTCAC13939260.33496925881113243No Hit
GAACCG13706280.3293706016429745No Hit
CTATGC13579410.32632183507535406No Hit
TCCTCC13565530.32598828987192874No Hit
TATAAG13534650.32524622388621016No Hit
CCTCGG13511900.32469952695696475No Hit
AGTCAA13457640.3233956247424217No Hit
CAGCCT13415330.32237888860719643No Hit
TTCACA13323940.3201827289428564No Hit
CCTTAT13273150.3189622130291696No Hit
CAGTAG13217390.3176222648632476No Hit
ATTGAA13149240.3159845771389367No Hit
TAAGTC13024490.3129867555159317No Hit
ATTCGA13014530.31274741039877635No Hit
ACCGCT12974250.31177945645108385No Hit
AATCGG12916200.31038447813272363No Hit
TCCACG12860610.3090486151746246No Hit
GCGGCT12698800.3051602182462203No Hit
AGACGG12638240.30370492303588614No Hit
GAATAC12505040.30050404255344715No Hit
CATGAG12240380.29414409489216853No Hit
TGACAC11622650.27929965119534383No Hit
CTAGTT11505500.27648446239265817No Hit
TTCGTT11411620.27422846644902926No Hit
CGTGAA11298890.2715194930497398No Hit
TACGAA11202040.2691921261223808No Hit
ATCAGC11108770.26695078886564594No Hit
TACTCG10995820.26423652872682096No Hit
CATTAA10941910.2629410372342662No Hit
TCTCTG10910300.2621814288855433No Hit
GGCAGG10861270.261003206888141No Hit
ATGCCG10807330.2597069944765587No Hit
GGAGGC10727960.2577996830359342No Hit
AGAGGT10719900.2576059961238587No Hit
AAGTTG10695610.25702229201786436No Hit
GACCAT10611900.25501068762458384No Hit
GTTGGC10599980.2547242424642935No Hit
GATCCT10451660.251160018791956No Hit
CGAGAG10377650.24938151155092514No Hit
GTACGC10300020.24751601341390003No Hit
TCGGAC10223110.24566781733353682No Hit
GCATAA10151180.24393929381175122No Hit
GGATCG10106580.2428675275240877No Hit
TATTCT10105120.242832442798079No Hit
GACTTG10103800.2428007223608656No Hit
AGTGCA10010720.24056395092463875No Hit
GGTGGT9855740.2368396832281793No Hit
GGCCAG9650640.2319110001429823No Hit
GAGCCA9453200.22716639171615977No Hit
AATGTA9382190.2254599763778865No Hit
TTCCAT9231600.22184120316579575No Hit
ATCCAC9180410.22061107499840799No Hit
GGATTC8914860.2142297400726446No Hit
CTCGTC8531100.2050077438718879No Hit
AAGGCG8509820.2044963719749937No Hit
AGTAGG8225530.19766470296333766No Hit
CGTCAG8182810.19663811426806893No Hit
TCAAGC7999180.19222536890027409No Hit
TATGGT7888830.1895735884105057No Hit
AGCCTA7772130.1867692133932337No Hit
CGACAA7727430.18569504404214493No Hit
TCTCGC7378580.17731195728314456No Hit
GTTACA7186440.17269471121786054No Hit
ACGCTA7158860.17203194632517532No Hit
GGCGTT6975660.16762953413010906No Hit
AACAAT6825340.16401724918926647No Hit
AGGTGT6808840.1636207437240995No Hit
GCTGTC6718810.16145726572234284No Hit
CCGACT6167680.14821326226673764No Hit
ACGTAA5688210.1366912940616698No Hit
GAGTGA4928470.11843425999464116No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)