FastQCFastQC Report
Wed 27 Sep 2023
EGAF00001836020

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836020
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences409763691
Sequences flagged as poor quality0
Sequence length6
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTCA68129951.6626643964899273No Hit
CCTCGG49866301.216952626483443No Hit
CTTAGC43549251.062789382185646No Hit
CGTACT39316940.9595027783952678No Hit
TACGGC36690550.8954075435639318No Hit
AAGCGC36188170.8831473064801146No Hit
GGTACC35417880.8643489107969794No Hit
CTGTAT34742220.847859894936372No Hit
TAGAGC34497660.8418915769674674No Hit
AACTAG34469130.8411953220130478No Hit
ACAGCC34018720.8302033769019325No Hit
CCGTCG33694370.8222878390657604No Hit
GAGTCG31263040.7629529088754718No Hit
GCGCAG30920730.7545990696379197No Hit
GGCCGT30794830.751526567052521No Hit
GCCATA30467020.7435265903049473No Hit
ACTAAT30452040.743161013746335No Hit
CTGTTC30239800.7379814430654374No Hit
GATCAG29790680.7270209795137754No Hit
GTCTGC29522040.7204650057684101No Hit
ACACGA29278050.7145105982560079No Hit
TCCAGA28962890.7068193360255534No Hit
CCTTAT28405460.6932156416953009No Hit
TCTTCA27605000.673680967989914No Hit
TAGCTT27499560.6711077775800297No Hit
TACAGT27337300.6671479342956231No Hit
TGATAA27322800.6667940718056447No Hit
GGCTAC27147190.6625084309873615No Hit
CAGATC26798120.6539896186165504No Hit
AACCTT26479490.6462136734315974No Hit
CAGGAC26478500.6461895131650403No Hit
GCACAT24709540.6030192655600616No Hit
CCAGCG24686820.6024647996447299No Hit
GTGGTG24648840.601537923964083No Hit
GGACTA24562840.5994391533338663No Hit
ATGCCG24402570.5955278746256706No Hit
CGAATT24243520.5916463691752523No Hit
CAACGG24191990.590388815098798No Hit
TTACGT24119330.5886155979593615No Hit
CTGAGG23880000.5827749145299455No Hit
GAATAC23690850.5781588393589514No Hit
GCTCAA23364030.5701830228779348No Hit
AACATA23320680.5691250960544476No Hit
CTGGAG23304690.5687348711430853No Hit
GACTTG23221160.5666963791577131No Hit
CAGTAG22997650.5612417719070185No Hit
ATTCAT22931770.5596340159870339No Hit
TGCATA22897510.5587979243382988No Hit
CCGGAA22713960.5543185133013652No Hit
GTACTT22647610.5526992873558434No Hit
CATACG22253680.5430856976539681No Hit
TCATGT22093420.5391746629888688No Hit
AACTCT21921450.534977853857725No Hit
CATGAG21791160.5317982163529467No Hit
GAAGAT21745320.5306795227984219No Hit
CTATTA21604040.5272316819305496No Hit
TCTCTG21328520.5205078065347669No Hit
CCTGCT21268010.5190311017576226No Hit
CAAGGA21067260.5141319365946457No Hit
GGTAAT20963050.5115887634856354No Hit
ATCCGG20920650.5105540207563193No Hit
CAGTCC20656570.5041093306629748No Hit
GGCCAG20628490.5034240576478993No Hit
GAGCAC20619320.5032002701283751No Hit
TGTGAT20600780.5027478142273958No Hit
ATGGCC20547940.5014582905052952No Hit
CAGCCT20525600.5009130982276319No Hit
TTCTAC20344820.49650128712843916No Hit
GACACG20160520.4920035728592654No Hit
GTAAGA20116950.4909402770876544No Hit
GAGAAG19996450.4879995577743856No Hit
CACATT19915170.4860159754857343No Hit
GAGATA19501040.4759094187288546No Hit
TGGCAT19462180.4749610672557125No Hit
ACTTGA19109980.4663658693956854No Hit
TCGTGG19076940.46555955100472773No Hit
ACCGCT18954080.46256123752067624No Hit
CCATGA18907360.4614210681736562No Hit
GCTTCT18876990.4606799092894739No Hit
ACCAGG18773450.45815308706793156No Hit
AGTTAG18512260.45177892542948617No Hit
CGTTGC18429000.4497470226077205No Hit
GTTGGC18364790.44818002188485756No Hit
ACGAAC18324730.44720238524013095No Hit
GCTGTC18054690.4406122454612505No Hit
ATTGAA18021670.4398064151564859No Hit
CGAGCC17891750.43663580724627943No Hit
GTCGAG17325620.4228197954220399No Hit
TGCGAC17315620.42257575232550315No Hit
ACTAGC17172880.41909228116553643No Hit
TTGAAT17119000.41777737696139605No Hit
CGTGAA16785510.40963878373498935No Hit
AAGGCG15947160.38917943073682437No Hit
CTAGTT15555700.3796261196797937No Hit
CGAAGC15282910.3729688680493655No Hit
AGGACT15272870.37272384878044257No Hit
AATCGG15193480.37078639063703667No Hit
CCACTT15140030.36948198028604734No Hit
TACTCG14962790.3651565604430286No Hit
TCAAGC14868680.36285987086152055No Hit
TTAGTA14781130.3607232735513406No Hit
CTACCG14705110.35886805793146764No Hit
TCCACG14109650.34433626770508563No Hit
TTAGCG13558660.330889737129003No Hit
AGGTGT13317000.324992191658094No Hit
TGTGTG13103890.31979138922779765No Hit
GCCAAT13077520.31914784758223No Hit
TCAATG12937710.31573588104954864No Hit
ATTGCT12897310.31474994693953984No Hit
TATTGG12835910.3132515223268037No Hit
CATAAC12800800.31239468701486295No Hit
GACCAT12783270.3119668794666339No Hit
TCGGAC12480800.3045853079256844No Hit
ATACCT12400070.30261514800734257No Hit
CGAGAG12286690.29984818737880803No Hit
TATTCT12112090.295587194913275No Hit
TTCTGT11978160.29231872572135725No Hit
CGCTTG11973240.29219865651786114No Hit
CTATGC11821160.2884872491057291No Hit
AAGCAT11810450.28822587894933815No Hit
AAGAGG11609960.28333305890687127No Hit
CGACAA11548610.28183585450961784No Hit
CGATGT11483110.2802373722273016No Hit
GCATAA11361550.27727078434579994No Hit
TTCCAT11305970.2759143928152482No Hit
TGACCA11033700.26926983142584004No Hit
TAAGTC10889670.26575487870642006No Hit
GGCAGG10880160.2655227937216135No Hit
ACAGTG10856020.26493367368657367No Hit
AGTTCC10841630.26458249567065717No Hit
TTCACA10838250.2645000091040277No Hit
GGTGGT10769350.262818552168889No Hit
GACCGA10753400.2624293034299127No Hit
ATGTGA10736330.26201272186412433No Hit
TGACAC10550140.25746888344970514No Hit
CCTGAC10491520.25603830281780626No Hit
TATAAG10482100.2558084142208686No Hit
AGGTCA10292810.25118892244652297No Hit
CTCCGC10252020.25019347065574926No Hit
TACGAA10178630.2484024383702655No Hit
GGATTC10154350.24780990173187403No Hit
AGAACG10142150.2475121691540991No Hit
GTACGC9892830.24142768667124292No Hit
CTCGTC9875520.24100524807113768No Hit
GGCGTT9828520.23985824551741455No Hit
AGACGG9740460.23770920200931128No Hit
TAATGA9679200.23621419399992666No Hit
CGTCTC9627440.23495102693225203No Hit
TTAGGC9444970.23049797254974455No Hit
TCTCGC9418990.22986394858494183No Hit
AGTGCA9386540.22907202873667984No Hit
CGTCAG9377410.22884921738954173No Hit
CAAGCT9148570.22326453516839295No Hit
AGTCAA9128700.22277962153557426No Hit
GATCCT9125130.2226924981501106No Hit
AGTAGG9057370.22103886212797708No Hit
GAGCCA9028760.2203406548287852No Hit
TTCGTT8863890.21631711629618253No Hit
GGATCG8847950.2159281116003028No Hit
ATTCGA8761050.2138073770913978No Hit
CACCTA8484010.20704640714494152No Hit
GTTACA8430240.20573418741486296No Hit
GGAGGC8329940.2032864351565986No Hit
AGAGGT8323030.20311780137689164No Hit
AATTGC8299420.20254161562596817No Hit
TATGGT8071620.19698231388685922No Hit
CTTCAC7666590.18709783634782812No Hit
CCGCTC7658570.18690211378440555No Hit
AGGCTT7484420.1826521032582167No Hit
ATCCAC7318010.17859098208874735No Hit
AGCCTA7224230.17630234592942495No Hit
GCCTGG7151220.17452058728160957No Hit
GAACCG7146600.1744078393710096No Hit
ACGCTA6923070.16895274403412186No Hit
CCGACT6789170.16568500697149371No Hit
GACTAA6521620.1591556339236509No Hit
GCGGCT6313770.15408319816213292No Hit
AACAAT6248400.15248788844007166No Hit
GAGTGA6210150.1515544235958183No Hit
GCGTAT6155260.15021487103892764No Hit
ATCAGC6014430.1467780121103995No Hit
ACCTGC5671240.13840269708035208No Hit
GTCTCG5655490.1380183292033066No Hit
TCCTCC5449810.13299885079373713No Hit
CTGGGG5374350.1311573015872702No Hit
AGATGC5304880.12946193419562887No Hit
TGCGGA5166770.12609145498935875No Hit
TGCGGC5131010.12521875687614303No Hit
ACGTAA4830410.117882821394246No Hit
GATTCA4799460.1171275080104645No Hit
AAGTTG4660470.11373555301169912No Hit
GGGTCG4210060.10274360790058386No Hit
AATGTA4169590.1017559654888993No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)