FastQCFastQC Report
Thu 14 Sep 2023
EGAF00001836024

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836024
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences410440134
Sequences flagged as poor quality0
Sequence length6
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCTT47216181.150379217057755No Hit
GAGATA44428831.0824679732708595No Hit
AAGAGG42970441.0469356293505157No Hit
CAGCCT39698850.9672263190519278No Hit
AAGCGC39398150.9599000374558887No Hit
CCTCGG38933490.9485790198090132No Hit
CAGATC38450410.9368092156406908No Hit
AACCTT36998080.901424518100367No Hit
AACATA35995270.87699196589776No Hit
CGATGT35874180.8740417183471634No Hit
CCGTCG34886570.8499795002990619No Hit
CAGTCC34105420.8309474920890656No Hit
GGCCGT33489680.8159455478591185No Hit
TCGTGG33239150.8098416126138385No Hit
GGACTA32845660.8002545871890784No Hit
CGTACT32806150.7992919620282553No Hit
ACCAGG31979050.7791404239235532No Hit
GCCATA31885840.7768694471774049No Hit
TACGGC31564230.7690337124780298No Hit
TTCTAC31533840.7682932878099099No Hit
CTGGAG31197770.7601052483819724No Hit
TGATAA31049670.7564969267844552No Hit
CCAGCG30647570.746700126552439No Hit
AACTAG30314900.7385949250274828No Hit
CCTTAT30130720.7341075470947975No Hit
ACAGCC29743230.7246667062047104No Hit
TTGAAT29488960.7184716492661509No Hit
CATACG29436030.7171820580294421No Hit
ACTTGA29266710.7130567304609642No Hit
GGTACC29041170.7075616538025982No Hit
CCACTT28762830.7007801532391079No Hit
GAGTCG28526070.695011711500903No Hit
AACTCT28425730.6925670187993848No Hit
ATTCAT28338180.6904339427976115No Hit
CCTGCT27894840.679632367530608No Hit
ACTAGC27661550.6739484691816224No Hit
GATCAG27550890.6712523390804662No Hit
AAGGCG27519430.6704858448369964No Hit
GTAAGA27233430.6635177153509068No Hit
TGTGAT27074550.6596467488727601No Hit
CATGAG26209260.638564746204863No Hit
CTGTAT26068600.6351376934303408No Hit
GGCTAC26051120.6347118091526595No Hit
AGGACT25565350.6228764655846253No Hit
TCATGT24875780.6060757206555244No Hit
CTGAGG24803920.6043249172119216No Hit
GTCGAG24647520.6005143736747732No Hit
CGAATT24603750.5994479574943322No Hit
TAGAGC24437750.5954035187017067No Hit
CAGTAG24349660.5932572860918128No Hit
GCTGTC24266490.591230924800351No Hit
GTTGGC24110860.5874391416118191No Hit
GTACTT23990570.5845083853325124No Hit
GAGCCA23990030.5844952287243917No Hit
AATCGG23905140.582426961199657No Hit
GTGGTG23803380.5799476714915993No Hit
CTTAGC23634940.57584378432154No Hit
GACACG23540520.5735433270275659No Hit
GAAGAT23388280.5698341381011244No Hit
GCGCAG23243520.5663071925612421No Hit
CAAGGA23143070.5638598198099214No Hit
TCAATG23056600.5617530570243894No Hit
CTGTTC23009790.5606125740130472No Hit
GGTAAT22975630.5597802967289743No Hit
TCCAGA22849260.5567014067878654No Hit
GCACAT22837130.5564058703869345No Hit
CGTTGC22779380.5549988442407048No Hit
GAGCAC22755660.5544209280469633No Hit
GTCTCA22713230.5533871597459326No Hit
GAATAC22392090.5455628761684402No Hit
GCTTCT22265180.5424708296192107No Hit
GCCAAT22251280.5421321687805511No Hit
ACTAAT22180020.5403959837904155No Hit
ACGAAC21905460.5337065794837695No Hit
CACATT21848320.5323144154318983No Hit
ATGCCG21728360.5293916993019986No Hit
TTAGTA21650750.5275008023460006No Hit
TACAGT21628850.5269672287944434No Hit
TGACCA21577790.5257231984043743No Hit
TCTTCA21311920.5192455180321134No Hit
TTACGT21278770.5184378484780438No Hit
CCGGAA21253880.5178314263000411No Hit
CGTGAA20616040.5022910357007143No Hit
ACACGA20450150.49824927695789123No Hit
GAGAAG20194190.49201304470873214No Hit
GGCCAG20112400.49002030586024514No Hit
CGAGCC19919300.48531560025267895No Hit
TGCATA19577880.47699721294799113No Hit
GACTTG19406250.4728156043336639No Hit
ATCCGG19387290.4723536612040966No Hit
CTACCG19359010.4716646447640035No Hit
GTCTGC19159540.4668047399087927No Hit
AGTTAG18840890.45904112291318955No Hit
ACAGTG18726620.4562570384503383No Hit
CAGGAC18441910.44932033863920334No Hit
CAACGG17747310.43239704234186804No Hit
CCATGA17552450.42764945593746445No Hit
TTAGGC17286120.4211605680842117No Hit
ATGGCC16692690.40670218668235797No Hit
ATTGAA16691370.4066700260847298No Hit
TGGCAT16402060.3996212514636787No Hit
CGAAGC16242510.395733961045827No Hit
ACCGCT16002940.3898970562172168No Hit
CGTCAG15850190.386175441605328No Hit
TAAGTC15612240.3803780066010796No Hit
CTATTA15600680.3800963577309426No Hit
GACCAT14931870.3638014112918109No Hit
TATAAG14791150.36037289667194194No Hit
GCTCAA14691690.35794964436884236No Hit
AGATGC14534890.3541293551960491No Hit
TTAGCG14467540.35248843379434236No Hit
CTAGTT14278700.34788751920639416No Hit
TCCACG14065530.3426938263303462No Hit
TCAAGC13739450.33474918415263943No Hit
AATGTA13574310.3307256984766504No Hit
TTCCAT13361150.32553224924149354No Hit
ATACCT12835890.31273476779441844No Hit
ATTGCT12788870.31158916832436273No Hit
TATTCT12630060.307719907332454No Hit
TCTCTG12612510.30729231756853487No Hit
CTATGC12385020.3017497309364001No Hit
CTCGTC12310560.29993558086110556No Hit
ATCCAC12309360.29990634395417093No Hit
GGAGGC11950080.29115281401793913No Hit
GTACGC11544370.2812680594242277No Hit
TACTCG11467350.27939153728080596No Hit
AAGCAT11054090.2693228338142975No Hit
GGATTC10928270.2662573441222003No Hit
TGTGTG10764240.26226090258512585No Hit
AAGTTG10729510.2614147377702591No Hit
TCTCGC10689030.2604284794429972No Hit
AGGCTT10655420.25960960240793607No Hit
AGCCTA10459270.2548305863285777No Hit
CATTAA10308460.25115623804956655No Hit
GAACCG10241780.24953164058756494No Hit
GCATAA10165610.24767582791988857No Hit
AATTGC10011620.2439240018375006No Hit
AGTCAA9989770.24339164649039902No Hit
CCGCTC9943630.24226748741876203No Hit
GTTACA9870510.24048598522287784No Hit
AGAACG9863700.24032006577602377No Hit
GATCCT9841530.23977991392040623No Hit
GGCGTT9786520.238439645378344No Hit
AGTTCC9754280.23765414714536662No Hit
ATGTGA9748250.23750723168802007No Hit
TAATGA9688840.23605976115386418No Hit
AGTGCA9595160.2337773332858331No Hit
TATGGT9580460.23341918117588376No Hit
GACCGA9562290.23297648567671506No Hit
TACGAA9542210.23248725476734206No Hit
CTCCGC9435720.22989272291778365No Hit
GCGTAT9312450.2268893616529226No Hit
ACGTAA9306020.22673270055993108No Hit
CCGACT9162280.22323060639094322No Hit
ACGCTA9017020.21969147880650486No Hit
TTCGTT8897230.2167729045717542No Hit
CGACAA8827340.21507009838370242No Hit
AGGTGT8793710.21425073406685907No Hit
AGTAGG8779650.21390817497394152No Hit
CGTCTC8632850.2103315266922703No Hit
CCTGAC8558870.20852907137974963No Hit
GGCAGG8551520.20834999532477494No Hit
TGACAC8439820.20562852657094202No Hit
TCGGAC8413340.20498336549125093No Hit
CGAGAG8405210.20478528544676874No Hit
AGACGG8219080.2002503975403146No Hit
TGCGAC8211720.20007107784444883No Hit
TTCTGT8207340.19996436313413735No Hit
ATCAGC8004450.19502113309416277No Hit
CACCTA7937590.19339215009612096No Hit
AACAAT7865380.19163281922132888No Hit
GGTGGT7625510.18578860516598505No Hit
GAGTGA7483060.18231794067195192No Hit
GATTCA7279500.17735838669227216No Hit
TTCACA7227210.17608438847259514No Hit
AGGTCA7220240.1759145707714831No Hit
TATTGG7146990.1741299012440143No Hit
CTTCAC7100450.17299599653673245No Hit
GGATCG6912060.16840604578888477No Hit
CGCTTG6830460.1664179361173291No Hit
ACCTGC6542040.1593908455355879No Hit
TCCTCC6461430.1574268563122533No Hit
GCCTGG6361740.15499800026865793No Hit
GACTAA6283170.15308371378711225No Hit
TGCGGA6118270.1490660754925102No Hit
AGAGGT6067370.14782594335669913No Hit
GCGGCT5943260.14480211625698378No Hit
CATAAC5451310.13281620261823615No Hit
ATTCGA4686480.11418181634255094No Hit
CAAGCT4258290.10374935702559732No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)