FastQCFastQC Report
Thu 14 Sep 2023
EGAF00001836026

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836026
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences410953187
Sequences flagged as poor quality0
Sequence length6
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTAGTA64362591.5661781447627514No Hit
ACTAAT53980851.3135522903244938No Hit
GGCTAC44919201.0930490727645823No Hit
GATCAG42866091.0430893677434847No Hit
CCTGCT42646551.0377471534245577No Hit
ACTTGA38600530.9392926304280005No Hit
TCGTGG38479660.9363514195109527No Hit
CAGATC38120640.9276151446417666No Hit
CCTTAT37646060.9160668706530799No Hit
CATGAG37316940.9080581725723421No Hit
GACACG34849960.8480274907808416No Hit
GGCCGT34559390.840956855750093No Hit
AGGACT33522300.8157206480065574No Hit
GCACAT32213510.7838729816201668No Hit
TAGCTT31864820.7753880735812374No Hit
ATTGAA31489410.7662529698303568No Hit
GCCAAT30709490.7472746524776313No Hit
ACTAGC30566790.7438022375040007No Hit
GCTTCT30363310.7388508219550564No Hit
GTCTGC29503610.7179311642617824No Hit
TTACGT29018720.7061320101162764No Hit
CTGTAT28691560.6981710060323733No Hit
GTCTCA28513200.6938308523204129No Hit
TTGAAT28496930.6934349434793409No Hit
TGTGAT28022710.6818954296125218No Hit
CTTAGC27777460.675927596590217No Hit
GGCCAG27529110.6698843291851634No Hit
GCTCAA27281600.6638615020644675No Hit
CCGTCG27040320.6579902737194249No Hit
ATGCCG26483240.6444344718027457No Hit
CGTTGC26344750.6410645015876224No Hit
GAATAC26127870.6357870148358284No Hit
GCTGTC25973700.6320354926460273No Hit
TTCTAC25940340.6312237213529628No Hit
GACTTG25799460.6277955936621074No Hit
ACCAGG25509080.620729582029011No Hit
CCATGA25351590.6168972720486531No Hit
TACGGC25234520.6140485290846521No Hit
ATGGCC25139170.61172831347333No Hit
GGACTA24901500.605944929683682No Hit
GACCAT24829740.6041987453914064No Hit
AACTAG24803230.6035536597505448No Hit
GCCATA24379910.5932527297811174No Hit
TCAATG24367920.5929609690555825No Hit
AAGCGC23991260.5837954482148839No Hit
CGTACT23804000.5792387248234189No Hit
GTGGTG23603080.574349603474422No Hit
CGAAGC23277460.5664260732451748No Hit
AACTCT23266470.5661586461914944No Hit
CCTCGG23080380.561630393196099No Hit
CGATGT22856900.5561923042100656No Hit
GCGCAG22770210.554082818197003No Hit
CAGCCT22614570.5502955255095758No Hit
AAGAGG22148000.5389421642324433No Hit
GAGTCG21987090.5350266330943432No Hit
CAGTAG21984830.5349716389959521No Hit
GAAGAT21909680.5331429635560899No Hit
AAGGCG21909340.5331346901076595No Hit
GAGCAC21845620.5315841485370936No Hit
CAAGGA21783640.5300759475555545No Hit
TGATAA21765860.5296432948699824No Hit
GGTACC21467930.522393564014385No Hit
GGTAAT21411760.5210267416663202No Hit
TCCAGA21257730.5172786261905787No Hit
AGACGG20961900.5100799960458756No Hit
CTGTTC20851610.5073962353770479No Hit
AGTTAG20716180.5041007261977992No Hit
CAACGG20564380.5004068748102931No Hit
ATCCGG20553870.5001511279191029No Hit
CTATTA20368630.4956435585447838No Hit
CGTGAA20207890.49173216413089893No Hit
CCACTT19794610.4816755442268903No Hit
ATTCAT19676350.47879784419338256No Hit
TATAAG19504800.47462340278675097No Hit
TGACCA19488520.4742272506089605No Hit
CTGGAG19473900.4738714923264484No Hit
ACAGCC18727800.45571613975584035No Hit
GAGATA18702620.4551034178985452No Hit
CGAATT18700310.4550472071165614No Hit
TACAGT18617780.45303894917841336No Hit
AGATGC18477380.449622501650048No Hit
GAGAAG18312780.4456171792628049No Hit
AGGCTT17933570.43638960755887746No Hit
AGTGCA17923340.4361406740958065No Hit
CGCTTG17863570.43468625052906573No Hit
CAGTCC17370490.42268780361107167No Hit
AACCTT16942380.41227031535346137No Hit
CTGAGG16924760.41184155605538597No Hit
CTAGTT16861410.4103000179434063No Hit
TGCATA16737840.4072931061123514No Hit
TGTGTG16608100.40413605552595455No Hit
CTACCG16536990.4024056881203844No Hit
GTTGGC16435330.39993192703966063No Hit
TGGCAT16358320.3980579909701491No Hit
AATTGC16262440.39572487851274407No Hit
CTTCAC16101770.39181518745588895No Hit
CACATT16070530.39105500354715583No Hit
CACCTA16056040.3907024086419848No Hit
CCGCTC16015900.389725655053747No Hit
GGTGGT15913600.3872363204230364No Hit
CTCCGC15872660.38624009989731506No Hit
AACATA15819400.38494408853434686No Hit
ATACCT15762380.38355658256520586No Hit
CCAGCG15693540.3818814525947453No Hit
ACCTGC15607970.3797992202941597No Hit
CATAAC15504560.3772828752876906No Hit
TTCACA15399160.3747181062742312No Hit
ATTCGA15319070.37276922249540795No Hit
GTCGAG15114100.36778154977540056No Hit
TAGAGC15084410.3670590830580418No Hit
TACTCG14930950.36332483777525737No Hit
TCCTCC14849910.36135283700817245No Hit
GTACTT14723360.3582734108349913No Hit
TCTTCA14584970.3549058739870534No Hit
TCTCGC14572240.3545961063443462No Hit
GCATAA14463960.35196125635594594No Hit
TGCGAC14439110.3513565646103628No Hit
GTTACA14280640.3475004076315875No Hit
ACACGA14240180.34651586726835626No Hit
GATTCA14148360.34428154951868034No Hit
CCGACT13795710.3357002801392072No Hit
ACGAAC13651170.33218309120936446No Hit
TTCTGT13631340.3317005544964906No Hit
ACGCTA13548840.3296930265684982No Hit
ACAGTG13538820.32944920317651655No Hit
TGCGGA13461590.3275699136991971No Hit
AGCCTA13387180.3257592451765072No Hit
GTACGC13337190.324542804920503No Hit
TTCCAT13286730.3233149278387273No Hit
TCGGAC13268290.32286621492973117No Hit
CCGGAA13253770.32251289001440453No Hit
AACAAT13122970.31933004573584195No Hit
CGAGCC13113680.31910398592431405No Hit
TCATGT13107630.3189567672095946No Hit
GACTAA13060910.31781989806055455No Hit
AAGCAT12520050.3046587883013546No Hit
CTATGC12487040.3038555337934391No Hit
GAACCG12417350.30215972020190224No Hit
TCTCTG12382620.301314611778397No Hit
TATTCT12309070.299524870213502No Hit
AAGTTG12281220.2988471774523554No Hit
TTAGGC12277200.29874935609150055No Hit
CAAGCT12227480.29753948592689705No Hit
GCGTAT12163960.29599381109070216No Hit
CAGGAC12139620.29540152951776477No Hit
TTCGTT12046000.2931234111587508No Hit
ATGTGA11973950.29137017010163735No Hit
GACCGA11906640.2897322706491141No Hit
TATGGT11755850.286062996270181No Hit
CATACG11672760.28404111147579447No Hit
CATTAA11561280.28132839373745994No Hit
GGCGTT11517750.2802691490016356No Hit
ATTGCT11362460.2764903730993574No Hit
CGTCTC11295060.27485028361636726No Hit
AGAACG11170750.2718253648681401No Hit
ACCGCT10986860.2673506459508246No Hit
GGCAGG10781310.2623488597011416No Hit
ATCAGC10776690.262236438137174No Hit
GTAAGA10721160.2608851893390962No Hit
GAGTGA10706770.2605350278011106No Hit
GCCTGG10503720.25559407573106374No Hit
CCTGAC10238210.24913324251698768No Hit
AGGTGT10179470.24770388263225707No Hit
TAAGTC10179300.24769974590804186No Hit
AGAGGT10040180.2443144454796502No Hit
TACGAA9803170.2385471219134261No Hit
GATCCT9662020.23511242413116995No Hit
TCCACG9529130.23187872247843158No Hit
GGAGGC9524930.23177652105664287No Hit
CGTCAG9448320.22991231845587318No Hit
AGTAGG9294030.2261578762254495No Hit
CGACAA9182080.2234337216613434No Hit
GGATTC8921510.21709309678622835No Hit
GAGCCA8869730.21583309925760474No Hit
AATCGG8719270.21217185499038363No Hit
TAATGA8641840.21028769877869327No Hit
TTAGCG8599900.20926714458111748No Hit
AGTCAA8267190.20117108861842214No Hit
AGGTCA8152420.19837831309968645No Hit
TGACAC8056410.19604203726494035No Hit
CTCGTC7933220.1930443722291902No Hit
GCGGCT7847990.19097041337703508No Hit
AGTTCC7642500.18597008714766336No Hit
GGATCG7221800.17573291139849465No Hit
ATCCAC6918420.16835056203129045No Hit
CGAGAG6897380.16783858157547274No Hit
AATGTA5898840.14354043688192641No Hit
TATTGG5507340.13401380435090773No Hit
TCAAGC5264570.12810631883480197No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)