FastQCFastQC Report
Thu 14 Sep 2023
EGAF00001836028

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836028
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences414452814
Sequences flagged as poor quality0
Sequence length6
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGAAG50160651.2102861485216023No Hit
TCTTCA50021551.2069299160314062No Hit
ACCAGG48674111.1744186154807963No Hit
TTCTAC46893051.1314448452508274No Hit
AACCTT44276371.068309069316634No Hit
AACTCT43223721.042910520568935No Hit
CGTACT42764231.0318238543797171No Hit
GCTCAA42606451.0280169071309526No Hit
TACAGT41171530.9933948717259765No Hit
CTTAGC39200290.9458324005974779No Hit
CAAGGA38460480.9279821176458461No Hit
TCCAGA36556010.8820306863690399No Hit
TTACGT36306980.8760220409554271No Hit
GGCTAC36080730.8705630359165568No Hit
GGTACC36052440.8698804491649561No Hit
CTGTAT35938160.8671230785755987No Hit
CCTCGG34244010.8262462901265281No Hit
ACTTGA33100400.7986530403917104No Hit
TAGAGC31959830.7711331403820557No Hit
AGGACT31883660.7692952954591351No Hit
GAGTCG31172640.7521396633586375No Hit
GCGCAG30882320.7451347646055553No Hit
CTGTTC29481190.7113280210470473No Hit
AACATA29391750.70916999492251No Hit
TAGCTT29226070.7051724349011177No Hit
GCCATA29115190.7024971001885875No Hit
AAGCGC28648860.6912453971177525No Hit
CGTGAA28550560.6888735951494831No Hit
CAGTAG28274320.6822084214392619No Hit
GTCTGC28204350.6805201713505558No Hit
ACTAAT27773000.6701124726830785No Hit
GAGCAC27688380.6680707444780433No Hit
GCACAT26623640.6423804858036264No Hit
GTACTT26240060.6331253912055715No Hit
CAGTCC25482980.6148584142560557No Hit
ACAGCC25425250.6134654933239275No Hit
CCGGAA25423320.6134189258997286No Hit
CAGATC25321310.6109576083129212No Hit
TGCATA25265930.6096213886486002No Hit
CGAAGC25211460.60830712564543No Hit
TCATGT25199620.6080214477684787No Hit
GGCCGT25123500.6061848092555115No Hit
GTAAGA24967650.602424429430946No Hit
CCAGCG24677610.5954262865736025No Hit
CGATGT24393400.5885688111168188No Hit
GGACTA24311150.5865842667435719No Hit
AGTTAG23754750.5731593367827875No Hit
TACGGC23544310.5680817985711638No Hit
AACTAG23529510.5677247012249748No Hit
AGAACG23527750.5676822355946171No Hit
GTTGGC23372590.5639385042274077No Hit
TGATAA23193080.559607251212921No Hit
GGCCAG23050150.5561586077202989No Hit
GAAGAT22964130.5540831000365701No Hit
ACTAGC22804040.5502204166479613No Hit
GCCAAT22781360.5496731890931256No Hit
CAGCCT22702760.5477767126464728No Hit
CGAGCC22695470.5476008180752755No Hit
ATTGAA22368660.5397154813382447No Hit
CTGAGG22322360.5385983457214505No Hit
CTGGAG22304550.5381686224960702No Hit
CAGGAC22269370.5373197924528992No Hit
GATCAG22121090.5337420630952695No Hit
ATGCCG21918870.5288628586799751No Hit
GACTTG21817070.5264066080149719No Hit
CCATGA21757480.5249688086325793No Hit
CTATTA21403870.5164368361605575No Hit
CGAATT21208520.5117233924728521No Hit
CATGAG21010590.5069476980315545No Hit
GTCTCA20534880.4954696724534726No Hit
GAGATA20416180.4926056552242398No Hit
CCACTT20394060.49207193946088157No Hit
AAGGCG20342760.49083416284875314No Hit
TTGAAT20197510.487329541934296No Hit
AGACGG20121910.4855054500848437No Hit
TCAAGC20059930.4840099843066816No Hit
ACGAAC20045940.48367243080173655No Hit
TGTGAT19651330.47415120216797463No Hit
GCTTCT19574560.4722988803256141No Hit
GTCGAG19483230.47009525190484047No Hit
ATCCGG19403640.46817488854111144No Hit
TGTGTG19271480.46498610575243915No Hit
TTCACA19075020.4602458797637697No Hit
ACACGA18970690.45772858475512723No Hit
CGTTGC18716580.451597368090255No Hit
CATACG18624080.4493655096765732No Hit
TGGCAT18588360.44850365040590606No Hit
GACACG17987340.43400212020275963No Hit
TCAATG17842730.4305129413357054No Hit
CCTTAT17677460.42652527387593026No Hit
CAACGG17628650.42534757647947835No Hit
GCTGTC17434650.4206667058605132No Hit
GGTAAT17117440.41301299983452394No Hit
GAACCG17080470.41212098031502326No Hit
CCGTCG16948390.4089341277822763No Hit
AATGTA16902010.40781506190955674No Hit
TTAGGC16712650.4032461461342617No Hit
ATTCAT16679480.40244581377121497No Hit
TCTCTG16235810.39174085569123435No Hit
TCGTGG16163660.39000000612856256No Hit
GCGTAT15976670.385488274185056No Hit
CTCCGC15953480.38492874124869614No Hit
TTAGCG15952230.3848985809998626No Hit
CTTCAC15674960.37820855524460256No Hit
TTAGTA15473350.3733440690307389No Hit
AATCGG15258140.36815143930956634No Hit
CGTCTC15200080.3667505560717462No Hit
GGTGGT15095280.3642219208095424No Hit
ACCGCT14939790.36047022713664095No Hit
GGCGTT14875910.3589289177802518No Hit
ATGGCC14742170.3557020124370539No Hit
TGCGAC14652010.3535266140091885No Hit
GTTACA14461160.348921747217284No Hit
TGACCA14452500.34871279701336516No Hit
TATTCT14236440.34349965832298585No Hit
GGATTC14183970.3422336517179493No Hit
GAATAC14109470.3404361008874704No Hit
GGATCG13872030.3347071013010422No Hit
AGTTCC13844330.334038750186891No Hit
TAATGA13711580.3308357317607693No Hit
CCTGAC13695250.33044171827000796No Hit
TGACAC13617840.3285739543802446No Hit
TAAGTC13536370.32660823000226996No Hit
AATTGC13529490.3264422279926901No Hit
CCTGCT13475590.32514171806298797No Hit
CACATT13116560.3164789707520239No Hit
GAGCCA13102830.3161476905788363No Hit
CTATGC12914640.3116070047964495No Hit
TCCACG12722070.30696063750214997No Hit
AGCCTA12709900.3066669973195067No Hit
TCTCGC12539960.3025666511700895No Hit
TACTCG12355800.298123202029942No Hit
GATTCA12354910.29810172793277256No Hit
AGAGGT12071710.2912686219570462No Hit
AAGCAT11859050.2861375191434941No Hit
GTACGC11753170.28358282542629815No Hit
TATGGT11623690.2804587062111249No Hit
GTGGTG11510310.2777230510009277No Hit
CTCGTC11438860.2759990911776027No Hit
AAGTTG11398270.27501972757748006No Hit
GATCCT11164790.26938627565935647No Hit
AAGAGG10980230.26493317523958226No Hit
ACAGTG10949670.26419581747609994No Hit
AGTAGG10853380.26187251318795485No Hit
ACGTAA10625760.25638045251636293No Hit
TCCTCC10592490.2555777073334095No Hit
ACCTGC10539960.2543102530364289No Hit
GACCGA10529370.25405473540831114No Hit
CGTCAG10500790.25336515147898114No Hit
GACTAA9934230.23969507901567777No Hit
ATTGCT9900150.23887278999148018No Hit
CGAGAG9841350.23745405188635055No Hit
TACGAA9443370.22785151122173344No Hit
CATTAA9422850.22735640057688208No Hit
TGCGGA9416290.22719811959100367No Hit
AGGTGT9009760.21738928282436515No Hit
TTCCAT8958290.2161474044183954No Hit
ATACCT8951260.21597778317895555No Hit
GGAGGC8857740.2137213140022256No Hit
ATGTGA8828970.21302714571507286No Hit
CATAAC8800890.21234962588527628No Hit
CTAGTT8765030.21148438866673974No Hit
AACAAT8753030.21119485027793777No Hit
CCGACT8663120.20902548389983908No Hit
TTCTGT8605420.20763328681368295No Hit
GACCAT8409030.20289474979894817No Hit
AGTCAA8221010.19835816581040272No Hit
GAGTGA8113600.19576655594863449No Hit
GCATAA7865760.18978662309191127No Hit
CCGCTC7819050.18865959491349962No Hit
GCGGCT7624270.1839599042992624No Hit
AGTGCA7617950.18380741408116003No Hit
TCGGAC7606850.1835395910715182No Hit
TATAAG7504700.1810748955368415No Hit
TTCGTT7479370.18046372825447868No Hit
CTACCG7478920.18045287056489862No Hit
AGGCTT7273190.17548897617087963No Hit
ACGCTA7196060.173627968176855No Hit
AGATGC7133580.17212043829915943No Hit
TATTGG7102350.1713669146423023No Hit
CACCTA6929350.16719273620374067No Hit
GGCAGG6883730.1660920077623119No Hit
CAAGCT6435240.15527075176282915No Hit
ATCCAC6007150.14494171102430975No Hit
ATCAGC5600060.1351193624661938No Hit
AGGTCA5383600.1298965724961877No Hit
CGACAA5034830.1214813805076493No Hit
ATTCGA4546440.10969740936540005No Hit
GCCTGG4385220.10580746111184566No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)