FastQCFastQC Report
Sat 16 Sep 2023
EGAF00001836032

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836032
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences399581933
Sequences flagged as poor quality0
Sequence length8
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCATCAA195047744.881295271175336No Hit
GGCCAATA52893541.3237220112251673No Hit
TCTACCGT52222701.3069334643816342No Hit
CTAACATA51866821.2980271557973568No Hit
CTCTATAG51034391.2771946323208763No Hit
TCGTCTGG47766051.195400643902486No Hit
CTCTTAAT47744881.1948708401688422No Hit
CCAGCCAA46447801.1624099130628112No Hit
CAGCGTCT44179581.1056450843086543No Hit
AATTGCTA43952811.0999699027933778No Hit
GTCAGATG42987941.0758229151466665No Hit
CCGGTCTT42440071.062111834771068No Hit
GAAGCGAT42279801.0581008926647342No Hit
TTGAGAGT41338461.0345427704810668No Hit
GGTGAGAT41218211.0315333751588813No Hit
AAGCGCAC39230460.9817876325254176No Hit
CAATATGG38502310.9635648366514109No Hit
TAAGCAAC38477310.9629391827382747No Hit
CTATGGAG37714510.9438492305406612No Hit
CTGCTCAG37590360.9407422332080264No Hit
AACCTTCT37564260.9400890505227123No Hit
GCTCACGT37229340.9317072901792085No Hit
TCGGTAAT36652870.917280461726982No Hit
ATAGGAGG35774440.8952967350503308No Hit
TCGAGCAA35615020.8913070651770435No Hit
CCTTACTG35361270.8849566779587105No Hit
ATCATGGA35065400.8775521890275254No Hit
ATGCTAGG35030490.876678525903222No Hit
CGACAGTT34821120.8714387995114883No Hit
TGAGGCGG34674350.8677657105182481No Hit
TAATGATT34432910.8617233952867432No Hit
AGCTGTGG33884060.84798778927775No Hit
GGAAGGCG33874110.8477387790203217No Hit
TGCCTAGA33742480.8444445860368768No Hit
GAGACCAT33359220.8348530612869326No Hit
CATTAAGT33242840.8319405171905008No Hit
CGCGCGCT32759900.8198543851580997No Hit
TAGCTGGT31385790.7854656932149132No Hit
GCAACAGC31351280.7846020405532198No Hit
GCCTGTGA31138180.7792689665976464No Hit
CTGGCCTC30156820.7547092976298305No Hit
CAGAGGAT30117360.7537217654933362No Hit
CATCCTCA30096890.7532094800692603No Hit
AGATGCGA29967570.749973097507389No Hit
AAGACACT29585250.7404050973445789No Hit
CTGTAGCA29466610.7374359941343994No Hit
GTTGGTCG29386350.7354273948116669No Hit
GGCGCCAT29250620.7320305945864675No Hit
CTCACCTT28771720.7200455682264293No Hit
CGTTCGCG28640770.7167683930294216No Hit
CGTCCGAA28464570.7123587842496372No Hit
TTGGAACG28066330.702392367674942No Hit
GGTCAACT27723240.6938061436326252No Hit
ATGCAACT27324470.6838264631949713No Hit
AACGCTGG27191820.6805067435318704No Hit
GTCATTAG26660190.6672020879382452No Hit
ATCTGTCT26356240.6595953876623346No Hit
GAGCCGTC26104960.6533068150506194No Hit
AGCACAAC25602460.6407311713965806No Hit
AGCATAGT24468220.6123455036191539No Hit
CGCTGAAG24307250.6083170432032522No Hit
CAAGCACT24066760.6022985028204465No Hit
TCAGGTAC23900280.5981321482820896No Hit
CGCGTGAA23854500.5969864508363545No Hit
CCGACCGC23399180.5855915412471865No Hit
GATTGGAT23361500.5846485556693075No Hit
CTGATCCT23250350.5818668983715037No Hit
ACATTCCT22647390.5667771270329182TruSeq Adapter, Index 27 (100% over 8bp)
AGGTGTAA22213050.5559072662076541No Hit
TAGAGTAG22022160.5511300231885109No Hit
CCAACAAT21614880.5409373701588255RNA PCR Primer, Index 36 (100% over 8bp)
GATTATCT20871290.5223281704280659No Hit
TGATAAGG20308640.5082472034590213No Hit
CTGTTCGC20279820.5075259496279577No Hit
GTGGCGAA20254400.5068897847290808No Hit
ATATTACA19918560.49848500032157356No Hit
TTGTACTC19875300.4974023687902826No Hit
TGGCCTAT19811650.49580945392743775No Hit
TCGCGGAT19607580.49070236616528906No Hit
AACTCTAC19220970.48102700379098473No Hit
GGAGAACC18693370.4678232036081571No Hit
CGAAGCAA18632690.4663046164301928No Hit
GGCCTGCT17503070.43803456949591363No Hit
GTCCTCAA16949690.42418559499785996No Hit
GAGAGCGG16553870.4142797417219562No Hit
GGATACTA16478210.4123862627192406No Hit
TAGTCGTG16417390.41086417187936275No Hit
CATAATAA16190210.405178729639911No Hit
CACTCTCG16059880.40191707065994897No Hit
GTTCGGAC15994830.4002891191779685No Hit
GCGCTGGC15532840.38872728512477567No Hit
CATCGAGC15490550.38766892896531435No Hit
TTCGTTCG15387240.3850834767346701No Hit
CCAGATTC13915320.34824697642172925No Hit
TATCTACT13430490.33611354495349516No Hit
TCTGCGCA13121600.32838321546434884No Hit
GGATCAGA12803600.32042489768925564No Hit
CTTCCTGT12101710.3028592886856073No Hit
ACTCGGAA11995320.30019675589286465No Hit
GAGGTGCG11288330.28250351349093655No Hit
TATTAGAG10837180.27121296297447967No Hit
CGTGCCAC10802900.2703550663287872No Hit
TTCACTTG10515300.2631575437120677No Hit
CTTAGATT10072140.25206695218624914No Hit
TCCTGGAA9929660.24850122540450298No Hit
AAGTTACG9923380.24834406114152313No Hit
TCGGCCAG9904780.24787857463014976No Hit
CGAACTGC9576970.23967475025954188No Hit
ATAACGCG9277850.2321889263196492No Hit
TATATAGC8957370.22416854367637287No Hit
GGCACGTT8954960.22410823063914653No Hit
GTCCAGAG8792780.22004948857384904No Hit
GTCTGGCG8698360.217686518874716No Hit
AGCCATTG8586150.21487833385099522No Hit
GCCACGAC8500540.21273584459085138No Hit
CAATTAAG8485320.21235494648853404No Hit
GTTGTGTG8352570.20903272420978053No Hit
TGTACGCC8103680.2028039641121612No Hit
GTATGCCT7991030.19998476757956923No Hit
TCTCTATA7874730.19707422557565935No Hit
GACGCTCA7853510.19654317053418932No Hit
ACGTGCGC7802010.1952543234731286No Hit
AACTACCT7659690.1916926008764265No Hit
AGACAGAG7565860.1893443966096435No Hit
CCTTAGCC7470970.18696966461694353No Hit
TTCGGAAG7444280.18630171649927926No Hit
TATAATCC7383980.18479263926079462No Hit
AGGATTGG7324240.1832975766699642No Hit
GATGTCCA7159210.17916751005856915No Hit
ACCTGAAC7091210.17746573141483854No Hit
ACCAGCGG6889030.17240594308852297No Hit
CTCCTTCA6822380.1707379497561017No Hit
ACGAGTTC6690520.16743800075665582No Hit
TGCTGCCG6639500.16616116625072735No Hit
GAGTAATT6393950.16001599351590304No Hit
ATATGGTT6308470.15787675765610754No Hit
CAGATTCA6096840.15258047215062648No Hit
CTCACGCC6016840.15057837962859047No Hit
TATAACGG5982090.14970872068933105No Hit
GGTGCGGA5946460.14881703873232927No Hit
CGGCCAAG5863870.14675012846489233No Hit
TAATTGAT5710040.1429003548065823No Hit
TGCCTGAC5661830.14169384380049035No Hit
CACGGCAA5645010.14127290384773228No Hit
AGGCGTGC5469960.13689207514795218No Hit
GAGTGGCA5326880.13331133267229078No Hit
AGCGCTTC5321680.13318119665835843No Hit
ATTGAGTA5287360.13232229896640496No Hit
CGCATATA5005480.12526792596501102No Hit
CTCAGAAC4974510.12449286589741784No Hit
TGGCTTCC4957140.12405816155857077No Hit
ACATCCAG4767330.11930794678847503No Hit
ATGTTGTG4754930.11899762244755945No Hit
TTGTCACA4730350.1183824795201639No Hit
AGTTCCGG4639840.11611736209304539No Hit
ACTTCTAT4599850.11511656609359262No Hit
TACGTCAG4430230.11087162942374573No Hit
GCCTCATT4415740.11050900041569196No Hit
TGCCAGGT4180000.1046093342763823No Hit
TGCATCCA4114940.10298113253283653No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)