FastQCFastQC Report
Fri 15 Sep 2023
EGAF00001836034

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836034
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences380213271
Sequences flagged as poor quality0
Sequence length8
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAGGTGA35857370.9430857030763663No Hit
CCATTGAC34700160.9126498901191694No Hit
ACGCTATT34111540.8971685788421625No Hit
GCAGATCT33598230.8836679980063085No Hit
TGTATATT33592180.883508876785103No Hit
GCAGTTAA31834470.8372792963347142No Hit
ACTGAGGC31420710.8263969828659663No Hit
GGCAACAC31247780.8218487460423233No Hit
TTAGCAGT29827030.7844815600873647No Hit
CGGACATC27840570.7322356194137158No Hit
CGTATGAC27374650.7199814443089232No Hit
TTATCGTC26693530.7020672879143137No Hit
GCCTACAT25018750.6580188517407116No Hit
GCCGGTTG24857610.6537807040407068No Hit
GTAATTCC24711190.6499297074772542No Hit
ATCTCAGT24308100.6393280259804502No Hit
GTGAGTCA24076780.6332440721144633No Hit
TCAATAAC23987360.6308922341640201No Hit
TCCGGTCT23968480.6303956707497461No Hit
GGTCGTAT23764270.6250247377609289No Hit
AATGCGAA23649630.6220095878767997No Hit
CTCCAATG23320500.6133531304329458No Hit
TAGCATGG23168320.6093506399464946No Hit
CCTGGCCA22997290.6048523750766186ABI Solid3 GAPDH Forward Primer (100% over 8bp)
GTATCTAG22938600.6033087677257851No Hit
GCACTCTC22540150.5928291230002858No Hit
CCGAATAG22468840.5909535966723265No Hit
TACATGTC22397360.5890735991695566No Hit
TTGTGTAC22284100.5860947447044793No Hit
CTACAAGC22027530.5793466898739577No Hit
CGTACACT21964760.5776957743276667No Hit
CTAGCGGC21820510.5739018509956219No Hit
TCCGCGAT21676150.5701050345504641No Hit
GATCGGCG21602360.5681642816723249No Hit
CGCCGGTA21519090.5659741950459167No Hit
CAGAGTGC21390890.5626024032180612No Hit
CCGCGTAC21347550.5614625166516084No Hit
GATCGATT21285000.5598173873315432RNA PCR Primer, Index 42 (100% over 8bp)
GCGGAGTA21265670.559308988454535No Hit
TCAAGGTT21171200.5568243303111847No Hit
GGCGTGTC20996720.5522353268936792No Hit
ATGCGGCA20982920.551872372703161No Hit
TGCTAACT20454210.5379667560315116No Hit
CAACAGCA20200120.53128392775117No Hit
TAGCCACC20133810.5295399065647027No Hit
ATGGCGCT20120470.529189050847202No Hit
GATCTTCC19952640.5247749492678808No Hit
ACCGAACT19855880.5222300617697271No Hit
GCAGTGGT19845320.521952322910896Clontech Universal Primer Mix Long (100% over 8bp)
GCGCCTAA19724510.5187748956821657No Hit
TTGACCGA19615890.5159180779883931No Hit
GACGATGT19339850.5086579421369013No Hit
CGACTCTG19256080.506454704996344No Hit
GCGACAAG19252130.5063508159345653No Hit
TGCTTCTT19140260.5034085198988227No Hit
AGGTAGCC19095310.5022262886768095No Hit
CATTGTGA19091040.5021139832859753No Hit
AGTCATGA19071330.5015955900182137No Hit
TGCTCAGC18998730.4996861353637496No Hit
GTTAAGCC18870070.4963022450628768No Hit
CTCTGAGA18813940.49482596834448733No Hit
ATGTCAAC18734180.4927281983274066No Hit
GTCTCCAC18710030.49209302849400016No Hit
ATACCGAA18688700.4915320275603952No Hit
ATTGCCAG18427550.4846635140202668No Hit
TAGGTCTC18350040.4826249213168574No Hit
CAGGAATC18147630.47730133017897736No Hit
AGACCATC18133800.4769375869576104No Hit
GTCCATCC18070330.4752682606915107No Hit
AATGGTCT18054260.4748456031667553No Hit
GTAATCTT18052370.4747958942232714No Hit
CGAATCGT18006080.4735784196233382No Hit
TAACTATG17881870.4703115688983933No Hit
CAGCTAAC17808860.46839133082232687No Hit
GTGGTCGT17745430.4667230565973591No Hit
GCTGCATG17707890.4657357159950369No Hit
CACAGGCA17706680.4657038917507958No Hit
ACTTGTCC17503070.46034873937895765No Hit
TTGCGTCG17451950.4590042308123432No Hit
GTGTTAAG17412030.4579542937626709No Hit
AGTCTACC17356040.4564816991882432No Hit
CTGTCATT17324390.45564927164259866No Hit
TAGCGCTG17015100.44751462660018515No Hit
GTTCTAAT16974940.4464583773037212No Hit
CAATACAT16898210.4444402994023846No Hit
TAAGACTG16853650.4432683255814077No Hit
GTAGAATG16831050.44267392234186376No Hit
GAGCTTAG16823260.44246903733141923No Hit
CGTTCATA16821110.44241249012057765No Hit
CCGGCTGA16805360.4419982489248777No Hit
GCTTCTCA16738400.4402371320700166No Hit
TTAGGATC16696750.439141694241388No Hit
CAGATAGT16691000.4389904633286722No Hit
ACGCATTA16657900.43811989929199496No Hit
TGTTGCGT16637610.43758625142782037No Hit
TTATGACG16618320.4370789045919442No Hit
GGTTCAAT16585460.43621465280205857No Hit
TGCAATCG16575580.43595479864247033No Hit
AGATGACT16464800.43304117072757303No Hit
ATGAATTG16387750.431014676497181No Hit
GTAGCACA16370840.43056992610865497No Hit
GCCATCGC16227070.42678862727019334No Hit
TGTCGGCT16212760.42641225955524314No Hit
ACACTGTG16123460.42406357772819564No Hit
CTGTAAGG16039890.42186560079329793No Hit
GAACGCGC16031290.42163941194993165No Hit
AGCCACGC15688490.4126234194492385No Hit
AGGATCTT15654370.411726028363697No Hit
AAGGCCTG15647810.41155349361805943No Hit
TGAGCTCC15604290.4104088728665129No Hit
AACAGTGA15266020.4015120240240115No Hit
TGCTCGAG15132320.3979955765405148No Hit
CATACGTT15096960.39706557217988325No Hit
TGATTCGC15045870.39572185264411774No Hit
AGAAGTAG14736200.38757721321095073No Hit
TCCGGCGA14517280.381819392095864No Hit
TCTCGAGT14517110.3818149209210533No Hit
ACCATAAG14508170.3815797897280655No Hit
CAATCGTA14361130.3777124865270681No Hit
GTTAATGG14290350.37585089974410707No Hit
CTCGTATC14251330.37482463361990326No Hit
TCATTGCG14245940.37468287107737486No Hit
GGATATGC14110730.3711267090411476No Hit
CTAGTCCG14049270.36951024784192765No Hit
TACAGCCT14037180.36919226840979996No Hit
TCCTCCTA14003380.3683032936533138No Hit
AATTCATT13997580.3681507476891831No Hit
CTTGCTTG13980030.36768916464254614No Hit
AGAACACA13936680.3665490150658103No Hit
AGGCGGTT13936160.3665353385310951No Hit
GACTAAGA13917660.3660487695075746No Hit
GTCATACA13792170.3627482534664078No Hit
AATATCAA13757220.3618290325273785No Hit
AGAAGAGC13722390.3609129677117451No Hit
TTACCTAC13656830.3591886722965017No Hit
ACATACTC13625790.35837228837811924No Hit
CTCTCGAA13588430.3573896819608908No Hit
CCACATAT13487440.35473354111303496No Hit
TCGTTCTG13459520.3539992164029435No Hit
TCGTCGAC13396770.3523488268772186No Hit
CGTGTTAG13070780.34377495466222163No Hit
AACACGTA13014470.3422939437587385No Hit
GCTGTAGC12999320.34189548318001767No Hit
CGATGGCA12940950.3403602921582398No Hit
CATTCCAG12920270.3398163868930288No Hit
GCCTGGTC12895060.339153337969626No Hit
TGAGTAAG12892860.33909547570736953No Hit
CCGCTGTA12872470.338559197740365No Hit
ATATTCGG12854680.3380913024469364No Hit
CAGTACCG12851340.33800345701241974No Hit
GAACAGAC12819510.3371662952816815No Hit
AGAACGGT12788500.336350700394148No Hit
AGCTCACG12721500.3345885314981549No Hit
GAGTCCTA12678130.3334478559008531No Hit
TTGTGGCT12657740.3329115779338486No Hit
GATACGAG12586860.33104736104805765No Hit
TGGCGCGA12449840.32744359415061025No Hit
TACGTACC12195760.3207610288805516No Hit
CTTCTAGA12189650.32060032959764834No Hit
CACACATG12062060.3172445813970549ABI Solid3 EF1 alpha Sense Primer (100% over 8bp)
TATAGGTG11974640.3149453455032084RNA PCR Primer, Index 39 (100% over 8bp)
AATTATTG11947340.3142273274306619No Hit
CGTTGTCT11919280.3134893205766087No Hit
TATACTAT11745130.30890899649844156No Hit
ACGGACGG11479450.3019213393001214No Hit
GTCGAAGC11446040.3010426219446717No Hit
AAGCTCCA11337680.29819264251825656No Hit
CTCGAACA11314060.29757141222984823No Hit
GCATAACG11299120.2971784748670701No Hit
ATACTTAG11258620.2961132832209847No Hit
GACAGTCG11205680.2947209067828671No Hit
CAAGAGAG11134540.2928498516297186No Hit
GGAACCTG11039280.2903444156740126No Hit
CAATCAGC10950310.28800441318630354No Hit
AAGTGGAG10866890.2858103814056506No Hit
TCCGTATG10803560.28414473728351264No Hit
TCGTGTCA10503990.2762657382361595No Hit
GAAGGCAG10143280.26677869431864204No Hit
GTCCGACT9982470.2625492259579756No Hit
AACGTCGA9784730.25734846062224903No Hit
CACATTAT9688010.25480462516522734No Hit
CGACCTCG9671260.25436408294122903No Hit
TACGAGCG9654370.253919858573269No Hit
CGGAGCTG9518280.25034055163213914No Hit
GAGACTGA9386770.24688170340061594TruSeq Adapter, Index 15 (100% over 8bp)
GCGCAATC9370370.24645036653652205No Hit
AGCGTGCG9235570.24290498792189716No Hit
TGGAAGAT9225940.24265170901938352No Hit
AAGCCTGT8115210.2134383678574965No Hit
AACTTGAA7461080.19623407621666106No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)