FastQCFastQC Report
Thu 14 Sep 2023
EGAF00001836070

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836070
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences377218766
Sequences flagged as poor quality0
Sequence length6
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAC48976091.2983471241194824No Hit
CCTTAT46358391.2289523793203863No Hit
GAAGAT44732111.1858399960939376No Hit
TAGAGC37337830.9898189953784007No Hit
TCGTGG36543220.9687540306518049No Hit
TTCTAC33215670.8805412931126549No Hit
GAGAAG33110960.8777654503010596No Hit
CGTACT31618260.8381942482681256No Hit
TGTGAT30814980.816899443438612No Hit
GTACTT30719520.8143688164230939No Hit
CCACTT30245270.8017965362836695No Hit
AACTCT30105230.7980841016801375No Hit
CTGAGG28655090.7596411574073173No Hit
AAGCGC28646250.7594068106357148No Hit
ACTTGA28516720.7559729941961583No Hit
CAGATC27189240.7207817439284026No Hit
AATCGG26964770.7148310855775399No Hit
ACCAGG26609870.7054227519529079No Hit
TACAGT26145170.6931036405542984No Hit
GACACG25815600.6843668005636814No Hit
CGTGAA25397060.6732713822620373No Hit
GGTAAT25076330.6647688890430228No Hit
GCTGTC24401750.646885897505958No Hit
TGATAA24318190.6446707372983665No Hit
GCGCAG23940670.6346627516405162No Hit
GATCAG23891270.6333531667403843No Hit
ATCCGG23728150.6290288855883697No Hit
TCCAGA23469800.6221800746784691No Hit
GTAAGA23454950.6217864039139559No Hit
TAGCTT23373200.619619226472948No Hit
AACATA23309260.6179241888511984No Hit
CAGGAC23249380.6163367810815648No Hit
CATACG23084440.6119642520648084No Hit
ACAGCC22983070.6092769520379588No Hit
GGCCGT22719630.6022932061656763No Hit
GCTCAA22618870.5996220771264599No Hit
CAGTAG22218760.589015234729865No Hit
CTGTAT22200840.5885401788308697No Hit
CACATT22126860.5865789826585669No Hit
GAGCAC21800760.5779341317287486No Hit
TCAATG21782890.577460401320543No Hit
GAATAC21644000.573778453005172No Hit
GCACAT21586750.5722607660510718No Hit
GCTTCT21363540.5663435100681072No Hit
GTCTCA21113870.5597248043592825No Hit
GGTACC20844970.5525963148927749No Hit
CATGAG20814420.551786440020325No Hit
ATTGAA20813760.5517689435419021No Hit
CAACGG20607720.5463068610960887No Hit
ATTCAT20571620.5453498567459923No Hit
GCCATA20419850.5413264620032185No Hit
TTAGTA20099290.5328284754528888No Hit
AACCTT19831460.5257283514892788No Hit
TTGAAT19763050.5239148149909382No Hit
CCGTCG19670530.521462126833849No Hit
TACGGC19565700.5186831028443585No Hit
CTATTA19532010.5177899871503211No Hit
CTTAGC19497510.5168753985054922No Hit
GTCTGC19356490.513136984282484No Hit
TTACGT18815470.49879464374261806No Hit
AACTAG18735960.49668684828898463No Hit
CAAGGA18348910.48642622408663516No Hit
ACACGA18097780.479768814046754No Hit
CGATGT17991760.47695824337647086No Hit
TGGCAT17880860.474018304805122No Hit
ACGAAC17587020.4662286605327583No Hit
ATGTGA17490180.4636614499714471No Hit
CCTCGG17384480.46085936244221737No Hit
AGGACT17271140.45785473992033576No Hit
GTTGGC16966990.4497917794471551No Hit
ACTAAT16643250.4412094916826063No Hit
CTGGAG16586100.43969445571008525No Hit
GCGTAT16408150.4349770339898732No Hit
GACTTG16313530.4324686752196204No Hit
GGCCAG16158840.4283678718147337No Hit
CGAAGC16065460.42589238521606315No Hit
CTGTTC15967470.423294688366591No Hit
CCAGCG15897620.4214429777335097No Hit
TCGGAC15885360.42111796739189805No Hit
TATGGT15881780.4210230622513621No Hit
CGTTGC15870610.42072694760896384No Hit
GCCAAT15782050.4183792383224116No Hit
GGACTA15762110.41785063259551625No Hit
TCTCTG15622440.4141480066238274No Hit
TCATGT15442550.40937915586097856No Hit
CCGCTC15430870.40906952121252627No Hit
CGAATT15378010.4076682123497536No Hit
CCGGAA15339720.40665315150307235No Hit
CAGCCT15050730.39899207983730056No Hit
CCATGA14856420.393840957530729No Hit
GTCGAG14735520.3906359208014587No Hit
TCTTCA14640710.38812252516620555No Hit
AGTTAG14480900.38388599150446295No Hit
AAGGCG14415390.3821493334719196No Hit
GAGATA14390670.3814940108255378No Hit
GTTACA14373500.38103883728838667No Hit
CCTGCT14288410.3787831170626331No Hit
TAATGA14247820.37770708363962996No Hit
CAGTCC14119020.37429261936560176No Hit
CGAGCC14066540.37290138423283004No Hit
ATGCCG13779950.36530393612495937No Hit
AAGAGG13693450.3630108370589389No Hit
GCATAA13663010.362203878266226No Hit
GACCGA13613390.3608884612066198No Hit
ACTAGC13520830.35843471265689897No Hit
GAACCG13375820.35459052426888005No Hit
TCCTCC13076490.34665534110781754No Hit
TGCGAC13058000.3461651746138208No Hit
GAGTCG13023930.3452619851897824No Hit
GACTAA12861810.34096421385355996No Hit
CTTCAC12734800.33759720214979977No Hit
CTCGTC12693980.3365150714691644No Hit
GTGGTG12617530.33448839605185493No Hit
CGTCTC12556250.33286387454011235No Hit
ACCTGC12317420.3265325352344745No Hit
AGAGGT12275190.3254130257135722No Hit
TGCATA12163710.32245771144906404No Hit
ATGGCC12046130.3193406873082237No Hit
TTCCAT11679550.3096227190351394No Hit
TAAGTC11633860.30841148555159636No Hit
AAGCAT11628080.30825825881631774No Hit
GTACGC11482340.30439471826277065No Hit
TACTCG11357060.3010735685403308No Hit
CTACCG11197690.29684869919753676No Hit
TTAGCG11141430.2953572569610707No Hit
AGTTCC10922690.28955849985469706No Hit
GGATTC10908230.28917516791834263No Hit
CACCTA10867350.2880914466487598No Hit
TCCACG10687810.2833318743214382No Hit
CGCTTG10510760.27863831143543905No Hit
GATCCT10497800.27829474422277284No Hit
CGAGAG10432920.2765747873741785No Hit
TATAAG10342370.27417432355419985No Hit
TGTGTG10296550.2729596437946038No Hit
GAGTGA10107080.2679368289964662No Hit
GACCAT10074060.2670614748790096No Hit
CATTAA10072430.2670182638792684No Hit
CGACAA10051980.26647613814631904No Hit
CGTCAG10047180.26634889103051673No Hit
AGACGG9963040.2641183551297657No Hit
TGCGGA9937280.26343546227496006No Hit
CATAAC9936430.2634129289315368No Hit
TTAGGC9838600.2608194736525913No Hit
ATACCT9750520.25848448907761923No Hit
GCGGCT9700330.2571539614230115No Hit
CTCCGC9695420.25702379822747207No Hit
AGGCTT9641390.2555914728802225No Hit
AGTAGG9624470.25514292679701944No Hit
AATGTA9521340.2524089694943756No Hit
GGAGGC9494180.25168896289746095No Hit
ACGCTA9388860.24889694909823232No Hit
CTAGTT9341270.24763534696468414No Hit
TTCTGT9218330.2443762302111979No Hit
AAAAAA9167040.24301654175921886No Hit
AGCCTA9103370.24132866178773302No Hit
TGACAC9016710.23903132115118578No Hit
AGTGCA8891640.2357157384900623No Hit
AGGTGT8538040.22634186762596006No Hit
TATTCT8503900.22543682251481623No Hit
GGCAGG8485010.22493605209450265No Hit
ACCGCT8466910.2244562244286648No Hit
AGAACG8296210.21993099887294576No Hit
ATTCGA8282150.21955827086290822No Hit
AATTGC8167620.21652210166023395No Hit
CTATGC8160400.21633070079021466No Hit
TACGAA8103370.21481884599558868No Hit
TTCACA8031920.21292471965723997No Hit
GGCGTT8029220.21285314315460116No Hit
AAGTTG8025250.21274789918590636No Hit
GGATCG7999430.21206341574215318No Hit
AGATGC7869340.20861475380575312No Hit
TTCGTT7832490.2076378670938126No Hit
ATTGCT7783400.20633650023657624No Hit
GATTCA7720270.20466293556561818No Hit
AGTCAA7633580.2023647996345972No Hit
GAGCCA7585690.2010952445563114No Hit
TATTGG7552290.20020981670885377No Hit
GGTGGT7390770.19592795126210663No Hit
ATCCAC7270790.1927473035633651No Hit
TCAAGC7259530.19244880303754558No Hit
AACAAT7106490.18839174082871582No Hit
AGGTCA7069060.18739947842361585No Hit
ACAGTG7068870.18739444155861537No Hit
ACGTAA7053870.1869967943217332No Hit
GCCTGG7034710.1864888662511557No Hit
CCTGAC6956570.18441738924515755No Hit
ATCAGC6882780.1824612299378552No Hit
TCTCGC6813220.18061720715135365No Hit
CCGACT6712090.1779362694802941No Hit
TGACCA6214920.1647563843629137No Hit
AAAACA5372890.1424343241714544No Hit
CCCCCC4750100.1259242759942648No Hit
AAAATA4203760.11144090323438469No Hit
CAAGCT4194000.11118216743225336No Hit
AGAAAA3938810.10441712754025603No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)