FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001836076

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836076
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24944889
Sequences flagged as poor quality0
Sequence length6
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATAAC8139563.263017125472076No Hit
TGGCAT7334562.940305727558058No Hit
GCATAA7029242.8179079089107195No Hit
CAAGCT6017072.4121454298714258No Hit
ACCTGC5642912.262150775655887No Hit
CACCTA5449352.1845557220158405No Hit
TAATGA5307782.1278026131926264No Hit
CTATGC4576401.8346042750480869No Hit
TAAGTC4502211.8048627115558622No Hit
AAGCAT4260141.7078207884589105No Hit
AGATGC4162651.6687386341947643No Hit
TCAAGC4137721.6587446029525326No Hit
TATGGT3697691.4823437378294206No Hit
GTTACA3645891.461577960920171No Hit
CCTGCT3421291.3715394764835394No Hit
AGGCTT3417581.3700521978670661No Hit
CTATTA3122791.251875684834677No Hit
GAGTGA3091391.2392879358974098No Hit
AATCGG3089271.2384380624022822No Hit
AGTAGG3049031.2223065013438223No Hit
GTAAGA2970871.190973429466854No Hit
ACGTAA2779491.114252302345382No Hit
AACCTT2601881.043051344104999No Hit
TATTCT2554621.0241055793032392No Hit
AGACGG2491500.9988017986369873No Hit
GATCAG2446250.9806618101207025No Hit
CTGGAG2340630.9383204711794869No Hit
TGCATA2167120.8687631362079824No Hit
GGCTAC2141930.8586648751974804No Hit
AGCCTA2044540.8196228093057459No Hit
GGACTA2040460.8179872037113494No Hit
GGATTC1978070.7929760681637027No Hit
CCTCGG1972960.790927552333466No Hit
ACTTGA1849920.7416028189181358No Hit
ACCGCT1822250.7305103662718243No Hit
CGATGT1814930.7275758974112893No Hit
GCACAT1758230.7048457902538673No Hit
GGTGGT1721350.6900611985084399No Hit
TGTGTG1698560.6809250584358183No Hit
CAACGG1623400.6507946377312002No Hit
CCATGA1621560.6500570116788252No Hit
GAGTCG1621010.649836525630561No Hit
AACTAG1615990.6478240893354947No Hit
GGCGTT1588270.6367115925029773No Hit
TGACCA1585530.6356131710988973No Hit
TACGGC1576300.6319130143252993No Hit
ACAGCC1555660.6236387742595286No Hit
AGTCAA1542590.6183992239853222No Hit
TTAGGC1537380.6163106197826738No Hit
AAGAGG1533270.6146629876765537No Hit
ATCCGG1477060.5921293135439488No Hit
ATCCAC1476250.5918045977274142No Hit
AACAAT1474610.5911471484198627No Hit
ACTAAT1455170.5833539688230323No Hit
ACGAAC1417740.5683488910293407No Hit
CCGACT1402970.5624278384241357No Hit
GCCAAT1396540.5598501560780648No Hit
TTAGTA1395590.5594693165401537No Hit
CTTAGC1394170.5589000616519079No Hit
CGAATT1393200.5585112044395146No Hit
TAGCTT1360370.5453501917767604No Hit
CACATT1357540.5442156908375099No Hit
ACAGTG1354450.542976960129989No Hit
CATACG1305530.5233657283461954No Hit
TCGTGG1284860.5150794617687013No Hit
CTGTTC1273080.5103570514986056No Hit
GACCGA1266250.5076190156628879No Hit
TGATAA1264360.5068613454243072No Hit
CAGATC1212550.4860915596778162No Hit
CATTAA1207070.4838947168696562No Hit
GATCCT1191830.4777852489141162No Hit
GAGATA1168330.46836448139737163No Hit
GGATCG1132490.45399680872502585No Hit
GGTAAT1122600.4500320686935107No Hit
TACAGT1121670.44965924683008207No Hit
CAAGGA1105770.4432851956166251No Hit
TTCGTT1091890.4377209295258841No Hit
TCTTCA1072360.4298916703938831No Hit
CGTCTC1024890.410861720010059No Hit
GAGCCA992150.39773678688247516No Hit
GAATAC986780.395584041283968No Hit
CTCCGC941020.3772396020683836No Hit
CGTACT933000.374024514600967No Hit
AGTTAG928130.3720722108645182No Hit
TATTGG916920.3675783043171689No Hit
CCAGCG904850.36273963776707924No Hit
CGAAGC904360.36254320474226204No Hit
AGGACT890580.3570190270239327No Hit
AAGCGC860980.3451528687900756No Hit
AATGTA855880.34310836179708No Hit
AACTCT855580.3429880966798449No Hit
CAGTCC850200.3408313422440966No Hit
AAGTTG843010.3379489882676968No Hit
CGAGAG836530.33535126173542No Hit
CTGTAT833540.3341526194003108No Hit
GAACCG828330.3320640151976623No Hit
TACTCG826540.3313464333314933No Hit
ATTGAA824010.3303321975094778No Hit
GTCTGC792180.3175720685708403No Hit
ATTCAT790060.3167221950757127No Hit
GGTACC781960.3134750369103667No Hit
TCTCTG774260.3103882322346674No Hit
CAGGAC773890.31023990525674416No Hit
ACTAGC772260.3095864647864338No Hit
ACACGA738350.2959924977016334No Hit
TTCTGT724750.290540479053645No Hit
CCGTCG723660.2901035157943577No Hit
TATAAG717910.28779843438068614No Hit
TAGAGC712800.28574991855044934No Hit
ATCAGC699940.2805945538583074No Hit
GCCATA699670.2804863152527959No Hit
TCCAGA694920.2785821175632411No Hit
GGCCGT680300.27272119751665364No Hit
TCAATG678860.2721439249539254No Hit
GACCAT669010.26819522027137505No Hit
GCTCAA666060.26701261328523046No Hit
CTAGTT665140.26664380025904305No Hit
GTCTCA645160.25863414345118957No Hit
TACGAA642370.2575156778609037No Hit
GTCGAG628480.25194740293292145No Hit
GCGCAG622940.24972650710131444No Hit
TTCCAT620580.24878042151239876No Hit
CGAGCC613620.2459902707925459No Hit
ATACCT602140.24138812563968515No Hit
GAGCAC574310.2302315315975148No Hit
GAAGAT573780.23001906322373294No Hit
AGGTGT568310.22782622925281407No Hit
TCTCGC566230.22699239110665115No Hit
CAGTAG563520.22590599621429464No Hit
CCTTAT534250.21417212960939616No Hit
ATGGCC532570.21349864495287993No Hit
TCGGAC517990.2076537602552571No Hit
GTACGC509780.20436250488025826No Hit
AGAGGT502980.20163649555626403No Hit
CTCGTC502120.20129173555352362No Hit
CGACAA500890.20079864857285995No Hit
GACTTG499950.20042181787219018No Hit
ATGCCG496060.19886238018537583No Hit
CGTCAG486050.19484953410696676No Hit
CCGGAA468400.18777393637630538No Hit
GGAGGC465030.18642295822603178No Hit
GTACTT444620.17824092141680806No Hit
GCTTCT433560.17380714742807635No Hit
ACGCTA420640.16862772971248738No Hit
CGTTGC417320.16729679574841966No Hit
TCCACG400960.16073833802186893No Hit
GGCCAG394640.1582047528854508No Hit
AGTGCA335830.13462878107014228No Hit
TTAGCG332580.1333259089667627No Hit
TTGAAT273670.10970984877904247No Hit
CTGAGG271930.10901231109907926No Hit
CTTCAC270120.10828671155842787No Hit
TTACGT267430.10720833434055368No Hit
TGCGAC260050.10424981245657175No Hit
TTCACA256110.10267033058355161No Hit
CAGCCT251710.10090644219743772No Hit
CCTGAC250780.1005336203340091No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)