FastQCFastQC Report
Tue 5 Sep 2023
EGAF00001836080

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836080
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences415266561
Sequences flagged as poor quality0
Sequence length6
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTACC51545571.241264643988515No Hit
GGCTAC48699391.1727260168198326No Hit
TTGAAT47018611.1322512914782945No Hit
TGTGAT43232891.0410876786199985No Hit
GCACAT39575140.9530057008370582No Hit
GGACTA37228010.8964846557919697No Hit
CGTGAA36236520.8726086664127044No Hit
AAGGCG35088180.8449555850464925No Hit
AACCTT35016580.8432313913183104No Hit
CTTAGC34925310.8410335259332378No Hit
GAAGAT34406950.8285509412832303No Hit
GGCCGT34311280.8262471198589958No Hit
GTCTCA34083600.8207643764507203No Hit
GATCAG34041250.8197445495737856No Hit
CCTTAT33851880.8151843461337596No Hit
ACTTGA33304900.8020125656108391No Hit
AACATA33005050.7947919023511262No Hit
CCACTT32884220.7918822050302288No Hit
TAGCTT32751930.7886965403891502No Hit
GCTGTC32651010.786266294145461No Hit
CAGCCT32474400.7820133632190048No Hit
GCCAAT32421510.780739723466441No Hit
TACGGC31765570.7649440861191806No Hit
TAGAGC31693810.7632160394441199No Hit
CTGAGG31661910.7624478581601951No Hit
AACTAG31336800.7546189109120202No Hit
TCATGT31200550.7513378858356958No Hit
TCCAGA30716720.7396868152839303No Hit
TTACGT30688190.7389997866936365No Hit
CACATT30374880.7314549942777598No Hit
ACTAGC30300880.7296730063463983No Hit
CATGAG29795100.7174933596447223No Hit
GAGCAC29604300.7128987204919686No Hit
GAGAAG29466930.7095907247874937No Hit
CTGTTC29461980.7094715242434365No Hit
TCGTGG29240140.7041294133962306No Hit
CCTGCT29051770.6995932908741959No Hit
TGACCA28624620.6893071267541814No Hit
GCCATA28559940.6877495729784995No Hit
GTGGTG28172680.6784239966771608No Hit
CGATGT27851320.6706853528714535No Hit
CCATGA27847630.6705964942840654No Hit
CAAGGA27842130.6704640492351129No Hit
CAGTCC27762520.6685469673538198No Hit
AGTTAG27739400.6679902165298592No Hit
GAGATA27431130.660566791940659No Hit
ACCAGG27291180.6571966674677665No Hit
CCGTCG27155400.6539269604228981No Hit
CAGGAC27004990.6503049495478159No Hit
TTAGTA26964240.6493236521396675No Hit
TGCATA26625610.6411691308802492No Hit
AAGCGC26596160.6404599478454033No Hit
ATGCCG26427770.6364049620648362No Hit
GTTGGC26332350.6341071608700996No Hit
CCTCGG26289780.6330820361912068No Hit
ACAGTG25906990.6238641015932896No Hit
GTAAGA25804630.6213991788276928No Hit
ATCCGG25470330.6133489279431772No Hit
CAGTAG25246600.6079613041609676No Hit
GGCCAG25066050.6036134944176254No Hit
TACAGT24978040.6014941328252048No Hit
TTCTAC24823420.5977707412853789No Hit
ACAGCC24778150.5966805981279095No Hit
ACCTGC24708630.5950064927091493No Hit
ATGGCC24691560.5945954314390366No Hit
GCTTCT24635510.5932456959856202No Hit
GGTAAT24597700.5923351964763665No Hit
GACACG24592880.592219126451648No Hit
ACACGA24400320.5875821048832295No Hit
GAATAC24364380.5867166366906196No Hit
CGTTGC23949320.5767216108691208No Hit
TTAGGC23936020.5764013346598356No Hit
CGAGCC23306700.5612467313495054No Hit
TCAATG22908780.5516644524623787No Hit
AACTCT22874620.5508418483037935No Hit
TCGGAC22849530.5502376580713899No Hit
ACGAAC22834690.549880297248398No Hit
CTATTA22749940.5478394394486292No Hit
AGGCTT22743780.5476911009938024No Hit
CTGGAG22706650.5467969765087828No Hit
GAGTCG22522670.5423665692167302No Hit
AGGACT22304320.5371085007733142No Hit
TCCTCC21553570.5190297515912917No Hit
CAACGG21497190.5176720694349382No Hit
AGTGCA21225290.5111244678330842No Hit
GTACTT20597550.4960079123731804No Hit
CAAGCT20589480.4958135793649901No Hit
TCCACG20497140.4935899473976668No Hit
TATGGT20390760.49102821934174473No Hit
CAGATC20386490.4909253938219216No Hit
GCGTAT20361800.49033083595671456No Hit
GACTAA20212800.4867427791760002No Hit
CCGCTC20077160.48347644345965046No Hit
GAGCCA19771120.47610671931757104No Hit
CGAAGC19675170.4737961552362989No Hit
TAAGTC19455430.46850461431687485No Hit
CATAAC19452870.46844296716681694No Hit
CACCTA19312460.4650617654716484No Hit
ATCCAC19135770.460806908071753No Hit
AGATGC19129150.4606474923946501No Hit
TACGAA19054270.4588443132554562No Hit
TTCCAT18746240.4514266680865739No Hit
ATTGCT18599060.4478824385766038No Hit
AAGAGG18588890.4476375356406315No Hit
AATTGC18204470.4383803491463884No Hit
AAGCAT18109190.4360859192801705No Hit
GACTTG18060330.43490932562711204No Hit
GTCGAG17693760.4260819835190149No Hit
GTTACA17687190.4259237718878116No Hit
ACGTAA17673920.4256042181060661No Hit
TGTGTG17629740.4245403231492073No Hit
CGAATT17445280.4200983570165189No Hit
GTACGC17392340.41882351321805555No Hit
AGTCAA17277990.4160698602457423No Hit
TATTCT17225660.4148097058072538No Hit
TGCGGA17206410.41434614813591986No Hit
AGACGG17126840.41243002949134644No Hit
GGCGTT17101240.41181355799076724No Hit
CGCTTG17041160.41036677643784564No Hit
GATCCT16987160.40906640686631157No Hit
GACCGA16970010.40865341912275954No Hit
TCAAGC16942790.40799793653503436No Hit
AATGTA16526580.39797521765784555No Hit
CTCGTC16512210.39762917486630955No Hit
TGGCAT16494220.39719595915164474No Hit
CATTAA16192060.38991966897137187No Hit
CTTCAC16048290.3864575553917523No Hit
AAGTTG15705680.37820719207872844No Hit
TACTCG15547020.3743865136302174No Hit
TAATGA15453240.37212820514098655No Hit
GATTCA15375870.3702650645159941No Hit
CTCCGC15259760.36746902912801593No Hit
GAGTGA15242160.3670452049713678No Hit
TATTGG15216950.3664381250288053No Hit
GCGGCT15185080.3656706661724203No Hit
CGAGAG15004140.3613134648710615No Hit
TTAGCG14886030.3584692676471006No Hit
GAACCG14740250.35495875142231836No Hit
AGTTCC14736500.35486844797985073No Hit
AGCCTA14491340.34896477012508603No Hit
CCTGAC14484690.3488046320204434No Hit
AGGTGT14128390.3402246009401176No Hit
ATTCGA14126160.34017090049299686No Hit
GGCAGG13602310.32755611160321674No Hit
TCTCGC13584990.3271390301036061No Hit
GGATTC13517290.3255087519555903No Hit
TTCGTT13448080.3238421116214074No Hit
GGATCG13407490.3228646671601376No Hit
GCATAA13251960.3191193619849396No Hit
GGTGGT13249240.31905386188800305No Hit
GCCTGG13048420.3142179319369758No Hit
ACCGCT12973550.3124149936069618No Hit
AGTAGG12892270.31045769659262307No Hit
CGTCAG12784420.3078605695872536No Hit
ACGCTA12747820.30697920798876943No Hit
CATACG12667610.30504767755668144No Hit
CCGACT12151060.29260867936823837No Hit
TGACAC11930900.28730702446325795No Hit
AACAAT11229180.27040896269035253No Hit
CTATGC11187810.2694127351130495No Hit
CGTCTC10971670.26420788549839436No Hit
CGACAA10716520.2580636392728959No Hit
AGAGGT10630670.2559962924633366No Hit
GGAGGC10487260.2525428480141939No Hit
AATCGG9979050.24030468468179889No Hit
TCTCTG7914630.19059155596205107No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)