FastQCFastQC Report
Fri 15 Sep 2023
EGAF00001836082

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836082
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences386702521
Sequences flagged as poor quality0
Sequence length6
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTACC45578601.178647604420453No Hit
GGCTAC42948131.1106245154269372No Hit
TTGAAT40977511.0596649303974928No Hit
TGTGAT37901770.9801273056608804No Hit
GGACTA35568950.9198013477651985No Hit
GCACAT34489640.8918907461686809No Hit
CTTAGC33775060.8734119424062405No Hit
AACCTT33460990.8652901955092246No Hit
GGCCGT33417090.8641549559486839No Hit
GTCTCA32734040.8464915076154883No Hit
CCTTAT32304390.8353808999347071No Hit
CGTGAA31777910.821766300302966No Hit
AAGGCG30945550.800241744480378No Hit
TACGGC30395750.7860240973189828No Hit
TAGAGC30327650.7842630537182352No Hit
GAAGAT29825420.7712755511102551No Hit
AACTAG29739240.7690469646563282No Hit
GATCAG29597080.7653707538151788No Hit
TCCAGA29288540.7573920108992515No Hit
GCTGTC29178680.7545510674340807No Hit
CACATT29110950.7527995919116338No Hit
CTGTTC28956480.7488050485194535No Hit
CCACTT28914300.7477142875931755No Hit
ACTTGA28704300.7422837566657602No Hit
TAGCTT28688520.7418756910560714No Hit
CAGCCT28519890.737514974721357No Hit
AACATA28389200.7341353743075287No Hit
CTGAGG28186500.7288936189790187No Hit
GCCAAT27449150.70982599050602No Hit
GCCATA27313740.7063243324446804No Hit
TCATGT27131670.7016160621306112No Hit
CAGTCC26880510.6951211471414224No Hit
TTACGT26696540.6903637434522958No Hit
CCGTCG26571360.6871266298261345No Hit
AGTTAG26558250.6867876095382373No Hit
ACTAGC26540940.6863399786317917No Hit
CAAGGA26523970.6859011400135144No Hit
GAGCAC26222650.6781091039228058No Hit
TCGTGG26194840.6773899464699895No Hit
GAGATA26193940.6773666727660148No Hit
CATGAG25998770.6723196407607593No Hit
GAGAAG25784850.6667877399226989No Hit
ATGCCG25727550.665305980769647No Hit
AAGCGC25701670.6646367324820207No Hit
CCTGCT25660390.6635692452597174No Hit
CCTCGG25658820.6635286455761171No Hit
TGCATA25445070.6580011408821408No Hit
GTGGTG25347850.6554870636594583No Hit
ATCCGG24621050.6366922547163845No Hit
GTAAGA24610310.6364145218489538No Hit
GGCCAG24343420.6295128342336305No Hit
CCATGA24310050.6286498970095931No Hit
CAGTAG24295860.6282829482769263No Hit
TGACCA24047920.6218713014286245No Hit
CAGGAC23942440.6191436233227969No Hit
ATGGCC23896100.617945286164814No Hit
ACCAGG23888660.617752890211957No Hit
TACAGT23739210.613888162368613No Hit
GGTAAT23605380.6104273625875845No Hit
ACAGCC23596510.6101979873050789No Hit
GTTGGC23554170.6091030888314276No Hit
CGATGT23532340.6085385722116872No Hit
TTAGTA23299630.6025207681539785No Hit
GAATAC23208270.6001582286038419No Hit
CTGGAG22145070.5726642263084702No Hit
ACGAAC21942110.5674157474654787No Hit
TCGGAC21930980.5671279293263258No Hit
ACAGTG21842670.5648442617729922No Hit
GAGTCG21819100.5642347493255675No Hit
GCTTCT21753100.5625280110340941No Hit
TTCTAC21696030.5610521996053912No Hit
GACACG21659500.5601075458207319No Hit
AACTCT21592170.5583664141667181No Hit
CGTTGC21447930.5546364152097162No Hit
ACCTGC21283410.5503819821231525No Hit
ACACGA21250410.5495286129774157No Hit
CAACGG20637490.5336787033772661No Hit
CGAGCC20601120.5327381871399799No Hit
TTAGGC20203610.5224587092878042No Hit
AGTGCA20150600.521087888123698No Hit
TCAATG20046270.518389948639616No Hit
AGGCTT19924570.5152428266688233No Hit
GTACTT19798170.5119741642439408No Hit
CTATTA19588440.5065506154277178No Hit
AGGACT19432390.5025152137552267No Hit
CGAAGC18940910.4898057026113879No Hit
TATGGT18875700.48811939346007005No Hit
TCCTCC18717750.4840348584125212No Hit
TAAGTC18202980.4707230755291611No Hit
TCCACG18152240.4694109558184132No Hit
ATCCAC18107550.4682552871177171No Hit
TACGAA17867340.4620435355268863No Hit
CAGATC17729600.4584816244319234No Hit
TTCCAT17664930.4568092795029904No Hit
CCGCTC17538390.45353699672415637No Hit
CAAGCT17511940.4528530084240128No Hit
GCGTAT17493440.45237460450897865No Hit
GACTTG17483100.4521072155099812No Hit
GACTAA17192370.4445890333360408No Hit
GTCGAG17129250.4429567708972862No Hit
GAGCCA17046300.4408117111809571No Hit
AAGCAT16963850.43867958130016954No Hit
CGAATT16737140.4328169352689583No Hit
CATAAC16690990.4316235114484811No Hit
CACCTA16492510.426490883931941No Hit
AGATGC16482630.42623539038164165No Hit
ACGTAA16474390.4260223066919183No Hit
GTTACA16451710.4254358093517575No Hit
GGCGTT16387050.42376372301953524No Hit
AGTCAA16244720.4200831160343017No Hit
AAGAGG16218200.41939731755718235No Hit
AGACGG16106230.4165018101860267No Hit
TATTCT16013930.4141149625450722No Hit
ATTGCT15805260.4087188249801971No Hit
TCAAGC15800200.40858797504451744No Hit
TGGCAT15582520.4029588418431852No Hit
TGTGTG15467150.39997541159034744No Hit
AATTGC15400210.3982443652080562No Hit
CTTCAC15315320.3960491377298262No Hit
GTACGC15029460.3886568921540597No Hit
CGCTTG14916290.38573035317760446No Hit
TACTCG14680180.37962462623821375No Hit
TGCGGA14665030.3792328522213073No Hit
CTCCGC14650200.3788493532991475No Hit
GACCGA14471630.37423159183386856No Hit
GATCCT14465780.3740803127580335No Hit
CATACG14454560.37379016724848296No Hit
CTCGTC14427100.3730800606805457No Hit
TAATGA14196760.3671235440433035No Hit
GAGTGA14191050.36697588532142No Hit
TTAGCG13992610.36184429219172326No Hit
AATGTA13978630.3614827739899839No Hit
GAACCG13952380.3608039576240569No Hit
AGTTCC13782370.356407554943248No Hit
CATTAA13753050.35564934938709647No Hit
CCTGAC13675420.3536418631209286No Hit
AGCCTA13543790.35023795461628243No Hit
AAGTTG13440600.34756949515723484No Hit
AGGTGT13356320.3453900420783655No Hit
GCGGCT13139710.3397885787250919No Hit
GATTCA13065870.33787910061232834No Hit
TCTCGC13025520.336835662884132No Hit
TATTGG13007600.33637225757832595No Hit
CGAGAG12905230.33372500304956637No Hit
GGCAGG12789850.33074131420001784No Hit
GGATTC12747600.329648743096764No Hit
GGTGGT12622960.32642559369298757No Hit
ACCGCT12260660.31705663485964186No Hit
GCATAA12194520.3153462762142169No Hit
ATTCGA11946440.30893100901196346No Hit
AGTAGG11854770.30656045296379125No Hit
TTCGTT11685530.3021839622297161No Hit
GGATCG11541300.29845422186942505No Hit
CCGACT11467470.2965450023533723No Hit
GCCTGG11310240.29247908626900315No Hit
TGACAC11244960.29079096694070944No Hit
CGTCAG11002840.28452982337811034No Hit
ACGCTA10858960.2808091339026983No Hit
CTATGC10464850.2706175789322046No Hit
AACAAT10456570.2704034608556379No Hit
CGTCTC10405230.2690758253422403No Hit
CGACAA10155270.262611941958351No Hit
AGAGGT9834430.25431512508810356No Hit
AATCGG9362030.24209901646852724No Hit
GGAGGC8901850.2301989130295843No Hit
TCTCTG6761130.17484059794893347No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)